Effects of Acanthopanax senticosus Polysaccharide on Immune Organ Indexes, Counts of Fecal Microflora and Gastrointestinal pH of Immune Challenged Weaner Piglets

HAN Jie, ZHANG Fei, BIAN Lianquan

doi:10.3969/j.issn.1006-267x.2014.08.036

2014 , Vol. 26 >Issue 8: 2314 - 2319

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.08.036

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Effects of Acanthopanax senticosus Polysaccharide on Immune Organ Indexes, Counts of Fecal Microflora and Gastrointestinal pH of Immune Challenged Weaner Piglets

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College of Animal Husbandry and Veterinary, Shenyang Agricultural University, Shenyang 110866, China

Received date: 2014-03-24

Online published: 2014-08-13

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Abstract

This experiment was conducted to evaluate the effect of dietary Acanthopanax senticosus polysaccharide (ASPS) on immune organ indexes, counts of fecal microflora and gastrointestinal pH of weaner piglets challenged with Escherichia coli lipopolysaccharide (LPS). A total of 64 Duroc×Large White×Landrace weaner piglets aged of 28 days with average initial body weight of (7.22±0.46) kg in a 2×2 factorial experiment were randomly allotted to 4 groups with 4 replications per group and 4 pigs per replication. Weaner piglets in 1 and 2 groups were fed basal diet, and those in 3, 4 groups were fed the basal diet added with 800 mg/kg ASPS. On 14 days, weaner piglets in 2 and 4 groups were injected with LPS and the other groups were given equivalent amount of saline. This experiment lasted for 21 days. The results showed as follows: compared with weaner piglets fed basal diet, dietary ASPS significantly increased the spleen, thymus indexes and the count of Lactobacillus in fecal by 20.06% (P=0.012), 35.38% (P=0.019) and 5.07% (P=0.043), respectively. Furthermore, ASPS supplementation significantly reducing effect on the count of Escherichia coli in fecal, the pH of ileum and cecum contents by 6.04% (P=0.036), 3.62% (P=0.041) and 2.74% (P=0.037), respectively. In addition, significant interactions were observed between ASPS and LPS in spleen (P=0.031), thymus indexes (P=0.047), the count of Lactobacillu in fecal (P=0.050) and the pH of cecum content (P=0.033). For the LPS challenge pigs, fed ASPS significantly reduced the count of Escherichia coli in fecal, the pH of ileum and cecum contents (P<0.05), while significantly increased the spleen and thymus indexes as well as the count of Lactobacillu in fecal compared with weaner piglets fed basal diet (P<0.05). It is concluded that dietary ASPS can improve the development of immune organ and modulate the health of intestinal tract of immune challenged weaner piglets.

Key words： Acanthopanax senticosus polysaccharide; weaner piglets; immune organ indexes; immune challenge; fecal microflora

Cite this article

HAN Jie, ZHANG Fei, BIAN Lianquan . Effects of Acanthopanax senticosus Polysaccharide on Immune Organ Indexes, Counts of Fecal Microflora and Gastrointestinal pH of Immune Challenged Weaner Piglets[J]. Chinese Journal of Animal Nutrition, 2014 , 26(8) : 2314 -2319 . DOI: 10.3969/j.issn.1006-267x.2014.08.036

References

[1] HEDEMANN M S, JENSEN B.Variations in enzyme activity in stomach and pancreatic tissue and digesta in piglets around weaning[J].Archives of Animal Nutrition, 2004, 58(1):47-59.

[2] MCGLONE J, POND W G.Pig production:biological principles and applications[M].Clifton Park, New York: Delmar Learning Inc., 2003:23.

[3] 林秋叶.刺五加水提物抗炎作用及其机制研究[D].博士学位论文.大连:大连理工大学, 2007:27-29.

[4] 韩杰, 边连全, 刘显军, 等.刺五加多糖对断奶仔猪生长性能和免疫指标的影响[J].动物营养学报, 2012, 24(11):2203-2209.

[5] 韩杰, 边连全, 刘显军, 等.刺五加多糖对断奶仔猪血液免疫指标的影响[J].动物营养学报, 2012, 24(12):2444-2449.

[6] 韩杰, 边连全, 张一然, 等.刺五加多糖对脂多糖免疫应激断奶仔猪生长性能和血液生理生化指标的影响[J].动物营养学报, 2013, 25(5):1054-1061.

[7] JOHNSON R W, VON BORELL E.Lipopolysaccharide-induced sickness behavior in pigs is inhibited by pretreatment with indomethacin[J].Journal of Animal Science, 1994, 72(2):309-314.

[8] CHANGHUA L, JINDONG Y, DEFA L, et al.Conjugated linoleic acid attenuates the production and gene expression of proinflammatory cytokines in weaned pigs challenged with lipopolysaccharide[J].The Journal of Nutrition, 2005, 135(2):239-244.

[9] 呙于明.动物免疫营养[M].北京:科学出版社, 2011:6-30.

[10] CHEN H L, LI D F, CHANG B Y, et al.Effects of lentinan on broiler splenocyte proliferation, interleukin-2 production, and signal transduction[J].Poultry Science, 2003, 82(5):760-766.

[11] 芦殿荣, 祝彼得, 芦殿香, 等.香菇多糖对正常小鼠以及免疫抑制小鼠免疫功能的影响[J].甘肃中医学院学报, 2004, 21(4):20-22, 12.

[12] 袁学千, 王淑梅, 高权国.刺五加多糖增强小鼠免疫功能的实验研究[J].中医药学报, 2004, 32(4):48-49.

[13] 张继东, 王志祥.中草药多糖的免疫调节机制及其应用[J].饲料工业, 2006, 27(6):1-4.

[14] 燕富永, 印遇龙, 孔祥峰, 等.刺五加提取物抗仔猪断奶应激的效用[J].中国农业科学, 2010, 43(21):4490-4496.

[15] 尹富贵, 印遇龙, 孔祥峰, 等.刺五加提取物对早期断奶仔猪肠道微生物多态性的影响[J].天然产物的研究与开发, 2007, 19(4):545-549.

[16] 王淑洁, 王长文, 杨连玉, 等.不同刺五加产品对哺乳犊牛消化道微生物区系的影响[J].中国兽医杂志, 2010, 46(3):54-55.

[17] 杨侃侃, 边连全, 刘显军, 等.刺五加多糖对断奶仔猪生长性能、血清免疫指标及粪便微生物菌群的影响[J].动物营养学报, 2013, 25(3):620-626.

[18] WANG J Q, YIN F G, ZHU C, et al.Evaluation of probiotic bacteria for their effects on the growth performance and intestinal microbiota of newly-weaned pigs fed fermented high-moisture maize[J].Livestock Science, 2012, 145(1/2/3):79-86.

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