Differential Analysis of Intestinal Microflora in Pigs with Different Growth Performance

HE Beibei, LI Tiantian, ZHU Yuhua, ZHOU Tianjiao, DAI Zhaolai, ZHANG Shihai, XUE Xinhe, ZANG Jianjun, WANG Junjun

doi:10.3969/j.issn.1006-267x.2014.08.038

Chinese Journal of Animal Nutrition >

2014 , Vol. 26 >Issue 8: 2327 - 2334

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.08.038

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Differential Analysis of Intestinal Microflora in Pigs with Different Growth Performance

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College of Animal Science and Technology, State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China

Received date: 2014-02-21

Online published: 2014-08-13

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Abstract

This experiment was conducted to analysis the differences of intestinal microbial composition in pigs with different growth performances by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Thirty-six barrows (Duroc×Landrace×Yorkshire) with a starting body weight of (26.6±2.6) kg were raised for 42 days (ad libitum) within single bar. Feed intake and body weight of each pig were recorded every week to calculate feed conversion rate and average daily gain. At the end of the experiment, feed conversion ratio and average daily gain were integrated to divide the pigs into 3 groups, namely, high growth performance group, middle growth performance group and low growth performance group. Three selected pigs from each group were killed to collect gut contents and feces. The results showed that the DGGE band numbers of ileum, colon, cecum and fecal samples were increased with the improvement of growth performance. Specifically, the abundance of Coprococcus sp. in cecum, Roseburia sp. in colon and fecal samples were higher in high growth performance group than those in the low growth performance group, while Roseburia sp. and Lactobacillus sp. were abundant in the gut of some high growth performance pigs. In summary, there is a correlation between pig gut microbiota composition and the growth performance of pigs. Bacteria belong to the genus of Coprococcus, Roseburia, Lactobacillus can serve as target for improving the health and growth performance of pigs.

Key words： pig; feed conversion ratio; average daily gain; microbial composition; PCR-DGGE

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HE Beibei, LI Tiantian, ZHU Yuhua, ZHOU Tianjiao, DAI Zhaolai, ZHANG Shihai, XUE Xinhe, ZANG Jianjun, WANG Junjun . Differential Analysis of Intestinal Microflora in Pigs with Different Growth Performance[J]. Chinese Journal of Animal Nutrition, 2014 , 26(8) : 2327 -2334 . DOI: 10.3969/j.issn.1006-267x.2014.08.038

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