Review

Probiotic Mechanism of Gram Negative Probiotics E. coli Nissle 1917 and Its Application in Piglets Raising

Expand
  • Key Laboratory for Bio-Feed and Animal Nutrition, College of Animal Science and Technology, Southwest University, Chongqing 400715, China

Received date: 2014-04-08

  Online published: 2014-09-10

Abstract

A gram-negative strain E. coli Nissle 1917 (EcN) is the most researched probiotic except lactic acid bacteria. EcN, serotyping of O6 : K5 : H1, has special genome, presents semi-rough O6-lipopolysaccharide(LPS) phenotype, K5 type and 3 different fimbriae (F1A, F1C and a kind of curli fimbriae capsule). The iron uptake system and the microcin of EcN, acting as their "fitness factor", are essential to antagonizing other bacteria. EcN has long-term steady colonization in the gut, interact with intestinal epithelial cells and develop immunomodulation biological function. This paper reviewed the futures, colonization mechanism, immunomodulation mechanism of EcN and its application in piglets raising, which will provide reference for further study on EcN and other probiotic gram-negative bacteria in animal intestine.

Cite this article

DENG Huan, LAI Xing, SUN Zhihong, CHEN Cheng, SHI Baoshi, TANG Zhiru . Probiotic Mechanism of Gram Negative Probiotics E. coli Nissle 1917 and Its Application in Piglets Raising[J]. Chinese Journal of Animal Nutrition, 2014 , 26(9) : 2476 -2482 . DOI: 10.3969/j.issn.1006-267x.2014.09.006

References

[1] SMITH F,CLARK J E,OVERMAN B L,et al.Early weaning stress impairs development of mucosal barrier function in the porcine intestine[J].American Journal of Physiolog:Gastrointestinal and Liver Physiology,2010,298(3):G352-G363.

[2] CUKROWSKA B,LODLNOVA-ZADNLKOVA R,ENDERS C,et al.Specific proliferative and antibody responses of premature infants to intestinal colonization with nonpathogenic probiotic E.coli strain Nissle 1917[J].Scandinavian Journal of Immunology,2002,55(2):204-209.  

[3] GROZDANOV L,RAASCH C,SCHULZE J,et al.Analysis of the genome structure of the nonpathogenic probiotic Escherichia coli strain Nissle 1917[J].Journal of Bacteriology,2004,186(16):5432-5441.  

[4] BLUM G,HACKER J,MARRE R.Properties of Escherichia coli strains of serotype O6[J].Infection,1995,23:234-236.

[5] LASARO M A,SALINGER N,ZHANG J,et al.F1C fimbriae play an important role in biofilm formation and intestinal colonization by the Escherichia coli commensal strain Nissle 1917[J].Applied and Environmental Microbiology,2009,75:246-251.

[6] VALDEBENITO M,CRUMBLISS A L,WINKELMANN G,et al.Environmental factors influence the production of enterobactin,salmochelin,aerobactin,and yersiniabactin in Escherichia coli strain Nissle 1917[J].International Journal of Medical Microbiology,2006,296:513-520.

[7] LÉVEILLÉ S,CAZA M,JOHNSON J R,et al.Iha from an Escherichia coli urinary tract infection outbreak clonal group A strain is expressed in vivo in the mouse urinary tract and functions as a catecholate siderophore receptor[J].Infection and Immunity,2006,74:3427-3436.

[8] HANCOCK V,VEJBORG R M,KLEMM P.Functional genomics of probiotic Escherichia coli Nissle 1917 and 83972,and UPEC strain CFT073:comparison of transcriptomes,growth and biofilm formation[J].Molecular Genetics and Genomics,2010,284:437-454.

[9] PATZER S I,BAQUERO M R,BRAVO D,et al.The colicin G,H and X determinants encode microcins M and H47,which might utilize the catecholate siderophore receptors FepA,Cir,Fiu and IroN[J].Microbiology,2003,149(9):2557-2570.  

[10] BURNS S M,HULL S I.Comparison of loss of serum resistance by defined lipopolysaccharide mutants and an acapsular mutant of uropathogenic Escherichia coli O75:K5[J].Infection and Immunity,1998,66:4244-4253.

[11] HUGHES C,PHILLIPS R,ROBERTS A P.Serum resistance among Escherichia coli strains causing urinary tract infection in relation to O type and the carriage of hemolysin,colicin,and antibiotic resistance determinants[J].Infection and Immunity,1982,35:270-275.

[12] HAFEZ M,HAYES K,GOLDRICK M,et al.The K5 capsule of Escherichia coli strain Nissle 1917 is important in mediating interactions with intestinal epithelial cells and chemokine induction[J].Infection and Immunity,2009,77:2995-3003.

[13] HAFEZ M,HAYES K,GOLDRICK M,et al.The K5 capsule of Escherichia coli strain Nissle 1917 is important in stimulating expression of Toll-like receptor 5,CD14,MyD88,and TRIF together with the induction of interleukin-8 expression via the mitogen-activated protein kinase pathway in epithelial cells[J].Infection and Immunity,2010,78:2153-2162.

[14] SPLICHAL I,FAGERHOL M K,TREBICHAVSKY I,et al.The effect of intestinal colonization of germ-free pigs with Escherichia coli on calprotectin levels in plasma,intestinal and bronchoalveolar lavages[J].Immunobiology,2005,209:681-687.

[15] BAQUERO F,MORENO F.The microcins[J].FEMS Microbiology Letters,2006,23:117-124.

[16] VASSILIADIS G,DESTOUMIEUX-GARZON D,LOMBARD C,et al.Isolation and characterization of two members of the siderophore-microcin family,microcins M and H47[J].Antimicrobial Agents and Chemotherapy,2010,54:288-297.

[17] WEHKAMP J,HARDER J,WEHKAMP K,et al.NF-κB- and AP-1-mediated induction of human beta defensin-2 in intestinal epithelial cells by Escherichia coli Nissle 1917:a novel effect of a probiotic bacterium[J].Infection and Immunity,2004,72:5750-5758.

[18] SCHLEE M,WEHKAMP J,ALTENHOEFER A,et al. Induction of human beta-defensing 2 by the probiotic Escherichia coli Nissle 1917 is mediated through flagellin[J].Infection and Immunity,2007,75:2399-2407.

[19] UKENA S N,SINGH A,DRINGENBERG U,et al.Probiotic Escherichia coli Nissle 1917 inhibits leaky gut by enhancing mucosal integrity[J].PLoS One,2007,2:e1308.

[20] ZYREK A A,CICHON C,HELMS S,et al.Molecular mechanisms underlying the probiotic effects of Escherichia coli Nissle 1917 involve ZO-2 and PKCζ redistribution resulting in tight junction and epithelial barrier repair[J].Cellular Microbiology,2007,9:804-816.

[21] CROSS M L,GANNER A,TEILAB D,et al.Patterns of cytokine induction by gram-positive and gram-negative probiotic bacteria[J].FEMS Immunology and Medical Microbiology,2004,42:173-180.

[22] UKENA S N,WESTENDORF A M,HANSEN W,et al.The host response to the probiotic Escherichia coli strain Nissle 1917:specific up-regulation of the proinflammatory chemokine MCP-1[J].BMC Medical Genetics,2005,6:43.

[23] SKAAR E P.The battle for iron between bacterial pathogens and their vertebrate hosts[J].PLoS Pathogens,2010,6:e1000949.

[24] STURM A,RILLING K,BAUMGART D C,et al.Escherichia coli Nissle 1917 distinctively modulates T-cell cycling and expansion via Toll-like receptor 2 signaling[J].Infection and Immunity,2005,73:1452-1465.

[25] GUZY C,PACLIK D,SCHIRBEL A,et al.The probiotic Escherichia coli strain Nissle 1917 induces γδ T cell apoptosis via caspase-and FasL-dependent pathways[J].International Immunology,2008,20:829-840.

[26] BICKERT T,TRUJILLO-VARGAS C M,DUECHS M,et al.Probiotic Escherichia coli Nissle 1917 suppresses allergen-induced Th2 responses in the airways[J].International Archives of Allergy and Immunology,2009,149:219-230.

[27] HELWIG U,LAMMERS K M,RIZZLLO F,et al.Lactobacilli,Bifidobacteria and E.coli Nissle induce pro-and anti-inflammatory cytokines in peripheral blood mononuclear cells[J].World Journal of Gastroenterology,2006,12:5978-5986.

[28] ARRIBAS B,RODRIGUEZ-CABEZAS M E,CAMUESCO D,et al.A probiotic strain of Escherichia coli Nissle 1917,given orally exerts local and systemic anti-inflammatory effects in lipopolysaccharide-induced sepsis in mice[J].British Journal of Pharmacology,2009,157:1024-1033.

[29] AGRAWAL S,AGRAWAL A,DOUGHTY B,et al.Cutting edge:different Toll-like receptor agonists instruct dendritic cells to induce distinct Th responses via differential modulation of extracellular signal-regulated kinase-mitogen-activated protein kinase and c-Fos[J].The Journal of Immunology,2003,171:4984-4989.

[30] GRABIG A,PACLIK D,GUZY C,et al.Escherichia coli strain Nissle 1917 ameliorates experimental colitis via toll-like receptor 2- and toll-like receptor 4-dependent pathways[J].Infection and Immunity,2006,74:4075-4082.

[31] LAMMERS K M,HELWIG U,SWENNEN E,et al.Effect of probiotic strains on interleukin 8 production by HT29/19A cells[J].The American Journal of Gastroenterology,2002,97:1182-1186.

[32] OTTE J M,MAHJURIAN-NAMARI R,BRAND S,et al.Probiotics regulate the expression of COX-2 in intestinal epithelial cells[J].Nutrition and Cancer,2008,61:103-113.

[33] KLETA S,STEINRUCK H,BREVES G,et al.Detection and distribution of probiotic Escherichia coli Nissle 1917 clones in swine herds in Germany[J].Journal of Applied Microbiology,2006,101(6):1357-1366.  

[34] GUNZER F,HENNIG-PAUKA I,WALDMANN K H,et al.Gnotobiotic piglets develop thrombotic microangiopathy after oral infection with enterohemorrhagic Escherichia coli[J].American Journal of Clinical Pathology,2002,118(3):364-375.  

[35] 唐志如,邓欢,孙卫忠,等.口服益生菌Escherichia coli Nissle 1917调控断奶仔猪空肠粘膜屏障功能的机理研究[J].畜牧兽医学报,2014,45(1):78-86.

[36] DUNCKER S C,LORENTZ A,SCHROEDER B,et al.Effect of orally administered probiotic E.coli strain Nissle 1917 on intestinal mucosal immune cells of healthy young pigs[J].Veterinary Immunology Immunopathology,2006,111:239-250.

[37] SPLICHALOVA A,TREBICHAVSKY I,RADA V,et al.Interference of Bifidobacterium choerinum or Escherichia coli Nissle 1917 with Salmonella Typhimurium in gnotobiotic piglets correlates with cytokine patterns in blood and intestine[J].Clinical and Experiment Immunology,2011,163:242-249.

[38] SCHROEDER B,DUNCKER S,BARTH S,et al.Preventive effects of the probiotic Escherichia coli strain Nissle 1917 on acute secretory diarrhea in a pig model of intestinal infection[J].Digestive Diseases and Sciences,2006,51:724-731.
Outlines

/