Shoots and Leaves of Sea Buckthorn: Effects on Rumen Fermentation Characteristics and Serum Immune Indices of Sheep

LIU Yanfeng, TANG Shuzhen, ZHANG Wenju, HOU Guangtian, HUANG Renhui, NIE Cunxi, WANG Wenqi

doi:10.3969/j.issn.1006-267x.2014.09.020

Chinese Journal of Animal Nutrition >

2014 , Vol. 26 >Issue 9: 2599 - 2606

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.09.020

Ruminants and Herbivorous Animal Nutrition

Shoots and Leaves of Sea Buckthorn: Effects on Rumen Fermentation Characteristics and Serum Immune Indices of Sheep

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1. Feed Research Institute, Xinjiang Academy of Animal Science, Urumqi 830000, China;
2. College of Animal Science and Technology, Shihezi University, Shihezi 832000, China;
3. Tiemenguan Animal Husbandry and Veterinary Station, The Xinjiang Production and Construction Corpssecond Division, Tiemenguan 841005, China

Received date: 2014-04-18

Online published: 2014-09-10

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Abstract

This experiment was conducted to study the effects of shoots and leaves of sea buckthorn on rumen fermentation characteristics and serum immune indices of sheep. A single factor random block design was used, and sixty healthy Altay lambs with the body weight of (28.0±3.5) kg were selected and randomly divided into 4 groups with 3 replicates in each group and 5 lambs per replicate. Lams in control (Con) group were fed a basal diet, and wheat straw in the basal diet was replaced by shoots and leaves of sea buckthorn at 2.5% (SJZY-1 group), 5.0% (SJZY-2 group) and 7.5% (SJZY-3 group), respectively, in test groups. Trial lasted for 63 days, which consisted of a 7-day-pretrial and a 56-day-formal trial. On day 56 of formal trial, two lambs per replicate in each group were randomly selected to collect blood and rumen fluid samples before morning feeding. The results showed as follows: 1) average daily gain in SJZY-1 and SJZY-3 groups was significantly higher than that in Con group (P<0.05). 2) Rumen fluid pH tended to be decreased with the increase of supplemental level of shoots and leaves of sea buckthorn, but no significant difference was found among groups (P>0.05); the concentrations of acetic acid, propionic acid, butyric acid and total volatile fatty acids (TVFA) in test groups were significantly higher than those in Con group (P<0.01), and SJZY-3 group had the highest values; acetic acid/propionic acid and acetic acid/TVFA were not significantly changed (P>0.05); compared with Con group, ammonia nitrogen (NH₃-N) concentration in SJZY-2 (P<0.05) and SJZY-3 groups (P<0.01) was significantly decreased. 3) Compared with Con group, the effects of the supplementation of shoots and leaves of sea buckthorn on serum CD4, interleukin-1 (IL-1) and interleukin-2 (IL-2) concentrations were low-dose inhibition and medium and high-dose stimulation, while the effect on serum γ-interferon (γ-IFN) was low-dose stimulation. The results indicate that shoots and leaves of sea buckthorn can improve average daily gain, increase volatile fatty acid concentrations and reduce NH₃-N concentration in rumen fluid, and increase serum CD4, IL-1, IL-2 and γ-IFN concentrations; the recommended supplemental level of shoots and leaves of sea buckthorn is 5.0% to 7.5% in diet for sheep.

Key words： shoots and leaves of sea buckthorn; Altay sheep; volatile fatty acid; ammonia nitrogen; cellular immune factor

Cite this article

LIU Yanfeng, TANG Shuzhen, ZHANG Wenju, HOU Guangtian, HUANG Renhui, NIE Cunxi, WANG Wenqi . Shoots and Leaves of Sea Buckthorn: Effects on Rumen Fermentation Characteristics and Serum Immune Indices of Sheep[J]. Chinese Journal of Animal Nutrition, 2014 , 26(9) : 2599 -2606 . DOI: 10.3969/j.issn.1006-267x.2014.09.020

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