Aquaculture Nutrition

Protective Effect of Water Soluble Material of Yeast Culture on Malondialdehyde Damaged Intestinal Mucosal Cells in Vitro of Grass Carp (Ctenopharyngodon idella)

Expand
  • 1. Key Laboratory of Aquatic Nutrition of Jiangsu Province, School of Biology and Basic Medical Sciences, Suzhou University, Suzhou 215123, China;
    2. Open Laboratory for Aquatic Animal Nutrition, Beijing Research Institute for Nutritional Resources, Beijing 100069, China

Received date: 2014-03-26

  Online published: 2014-09-10

Abstract

This experiment was carried out to explore the protective effect of water soluble material of yeast culture on malondialdehyde (MDA) damaged intestinal mucosal cells in vitro of grass carp (Ctenopharyngodon idella). MDA (4.94 and 9.89 μmol/L) and water soluble material of yeast culture with different concentrations were added in culture medium, in order to observe the changes of growth, morphology and relative enzyme activities of MDA damaged intestinal mucosal cells in vitro of grass carp under the different concentrations (50, 100 and 200 mg/L) and different action time (3, 6, 9 and 12 h) of water soluble material of yeast culture. The results showed as follows: added 4.94 or 9.89 μmol/L MDA in culture medium significantly inhibited the growth of cells (P<0.05), caused adherent cells decrease and cellular membrane permeability increase, and led to intracellular enzyme leakage and anti-oxidase activities of cells decrease. Cellular growth and cellular total protein content were significantly increased (P<0.05) and cellular growth conditions were improved on 9 h after water soluble material of yeast culture added in culture medium with the concentrations of 50, 100 and 200 mg/L. Water soluble material of yeast culture with the concentration of 100 mg/L had a better protective effect for cells which damaged by 4.94 μmol/L MDA, and there was no difference in cellular growth conditions compared with normal group. The concentration of MDA and leakage of intracellular enzyme were decreased in a certain extent, and the anti-oxidative ability of cells damaged by MDA was improved when adding 50, 100 and 200 mg/L water soluble material of yeast culture in culture medium. In conclusion, the intestinal mucosal cells in vitro of grass carp damaged by 4.94 and 9.89 μmol/L MDA can be protected by 50, 100 and 200 mg/L water soluble material of yeast culture within 12 h. Adding 100 mg/L water soluble material of yeast culture has the best protective effect for cells which damaged by 4.94 μmol/L MDA, and there was no difference in cellular growth conditions compared with normal group. Overall, the water soluble material of yeast culture plays the protective effect for intestinal mucosal cells in vitro of grass carp through enhancing cellular antioxidant capacity.

Cite this article

YAO Shibin, YE Yuantu, CAI Chunfang, ZHANG Baotong, XIAO Peizhen, CHEN Kequan, PENG Kan . Protective Effect of Water Soluble Material of Yeast Culture on Malondialdehyde Damaged Intestinal Mucosal Cells in Vitro of Grass Carp (Ctenopharyngodon idella)[J]. Chinese Journal of Animal Nutrition, 2014 , 26(9) : 2652 -2663 . DOI: 10.3969/j.issn.1006-267x.2014.09.027

References

[1] MLAKAR A,SPITELLER G.Previously unknown aldehydic lipid peroxidation compounds of arachidonic acid[J].Chemistry and Physics of Lipids,1996,79(1):47-53.  

[2] 姚仕彬,叶元土,李洁,等.鱼油在氧化过程中氧化指标及其脂肪酸组成的变化[J].饲料研究,2012(6):74-76.

[3] 殷永风,叶元土,蔡春芳,等.在自制氧化装置中氧化时间对豆油氧化指标的影响[J].安徽农业科学,2011,39(7):4052-4054.

[4] 任泽林,霍启光.氧化油脂对动物机体的影响[J].动物营养学报,2000,12(3):1-13.

[5] 王刚石,王孟薇,吴本俨,等.人胃黏膜细胞的分离与羟自由基损伤模型的建立[J].世界华人消化杂志,1999,7(11):1006-1008.

[6] 于会民,梁陈冲,陈宝江,等.黄曲霉毒素解毒酶制剂对饲喂黄曲霉毒素B1饲粮的断奶仔猪生长性能及肝脏生化指标的影响[J].动物营养学报,2013,25(4):805-811.

[7] 宋小珍,鲁琳,刘凤华,等.高温应激对仔猪小肠上皮脂质过氧化的动态影响[J].动物营养学报,2008,20(1):75-79.

[8] REQUENA J R,FU M X,AHMED M U,et al.Quantification of malondialdehyde and 4-hydroxynonenal adducts to lysine residues in native and oxidized human low-density lipoprotein[J].Biochemical Journal,1997,322(1):317-325.

[9] UCHIDA K.Role of reactive aldehyde in cardiovascular diseases[J].Free Radical Biology and Medicine,2000,28(12):1685-1696.  

[10] BUTTKUS H.The reaction of myosin with malonaldehyde[J].Journal of Food Science,1967,32(4):432-434.  

[11] ESTERBAUER H,SCHAUR R J,ZOLLNER H.Chemistry and biochemistry of 4-hydroxynonenal,malonaldehyde and related aldehydes[J].Free Radical Biology & Medicine,1991,11(1):81-128.  

[12] 杨玖英,吴士筠,谭艳平,等.线粒体丙二醛含量对膜流动性的影响[J].化学与生物工程,2004,21(2):13-15.

[13] 潘华珍,冯立明,许彩民,等.丙二醛对红细胞的作用[J].生物化学与生物物理进展,1984,2(1):34-35.

[14] 杨尚琪,李月白,李良晨,等.丙二醛对小鼠淋巴细胞的抑制作用[J].河南医科大学学报,1995,30(4):370-373.

[15] 李月白,杨尚琪,油书恒,等.丙二醛对巨噬细胞免疫功能的抑制作用[J].中国公共卫生学报,1995,14(4):254-255.

[16] MARNETT L J.Oxy radicals,lipid peroxidation and DNA damage[J].Toxicology,2002,181:219-222.

[17] FENG Z H,HU W W,MARNETT L J,et al.Malondialdehyde,a major endogenous lipid peroxidation product,sensitizes human cells to UV- and BPDE-induced killing and mutagenesis through inhibition of nucleotide excision repair[J].Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis,2006,601(1):125-136.

[18] FERRARIS R P,AHEARN G A.Sugar and amino acid transport in fish intestine[J].Comparative Biochemistry and Physiololgy,1984,77(3):397-413.  

[19] VERNIER J M.Intestine ultrastructure in relation to lipid and protein absorption in teleost fish[J].Comparative Physiology,1990,5(1):166-175.

[20] ANDREWS J S,GRIFFITH W H,MEAD J F,et al.Toxicity of air-oxidized soybean oil[J].The Journal of Nutrition,1960,70(2):199-210.

[21] 吉红,叶元土.氧化酸败油脂对水生动物致毒作用及其对策的研究进展[J].中国饲料,1999(17):13-15.

[22] 邱燕,叶元土,蔡春芳,等.酵母培养物对草鱼(Ctenopharyngodon idellus)生长性能与肠道黏膜形态的影响[J].饲料工业,2010,31(18):15-17.

[23] 李高锋,叶元土,林炳贤,等.酵母培养物对团头鲂生长的影响[J].饲料工业,2009,30(22):17-22.

[24] 许凡,叶元土,蔡春芳,等.注射硫代乙酰胺并饲喂酵母培养物、姜黄素和水飞蓟素对草鱼脂代谢相关基因表达丰度的影响[J].水产学报,2012,36(5):740-747.

[25] 刘哲,魏时来.酵母培养物对建鲤生长性能影响的研究[J].饲料工业,2003,24(4):52-53.

[26] 姚仕彬,叶元土,蔡春芳,等.草鱼肠道黏膜上皮细胞的分离与原代培养[J].上海海洋大学学报,2013,22(1):33-41.

[27] LONG J G,WANG X M,GAO H X,et al.Malonaldehyde acts as a mitochondrial toxin:inhibitory effects on respiratory function and enzyme activities in isolated rat liver mitochondria[J].Life Sciences,2006,79(15):1466-1472.  

[28] QUARONI A,WANDS J,TRELSTAD R L,et al.Epithelioid cell cultures from rat small intestine.Characterization by morphologic and immunologic criteria[J].The Journal of Cell Biology,1979,80(2):248-265.  

[29] 谭凤霞,王敏,王卫民.利用草鱼CIK细胞和MTT法测定镉和铬毒性试验的优化[J].水生生物学报,2006,30(3):371-374.

[30] BROWN S,LEVINSON W,SPUDICH J A.Cytoskeletal elements of chick embryo fibroblasts revealed by detergent extraction[J].Journal of Supramolecular Structure,1976,5(2):119-130.  

[31] BROWN K I,CRABO B G,GRAHAM E F,et al.Some factors affecting loss of intracellular enzymes from spermatozoa[J].Cryobiology,1971,8(2):220-224.  

[32] 冯琳,姜俊,刘扬,等.大豆凝集素对鲤鱼肠道上皮细胞结构和功能的影响[J].动物营养学报,2011,23(7):1140-1146.

[33] SIEMS W G,PIMENOV A M,ESTERBAUER H,et al.Metabolism of 4-hydroxynonenal,a cytotoxic lipid peroxidation product,in thymocytes as an effective secondary antioxidative defense mechanism[J].Journal of Biochemistry,1998,123(3):534-539.  

[34] POLI G,SCHAUR J R.4-hydroxynonenal in the pathomechanisms of oxidative stress[J].IUBMB Life,2000,50(4/5):315-321.

[35] SLATTER D A,BOLTON C H,BAILEY A J.The importance of lipid-derived malondialdehyde in diabetes mellitus[J].Diabetologia,2000,43(5):550-557.  

[36] SLATTER D A,AVERY N C,BAILEY A J.Identification of a new cross-link and unique histidine adduct from bovine serum albumin incubated with malondialdehyde[J].Journal of Biological Chemistry,2004,279(1):61-69.  

[37] DANIEL H,MORSE E L,ADIBI S A.Determinants of substrate affinity for the oligopeptide/H+ symporter in the renal brush border membrane[J].Journal of Biological Chemistry,1992,267(14):9565-9573.

[38] 谷瑞增,刘艳,林峰,等.蛋白水解物在动物细胞培养中的应用研究进展[J].生物技术通报,2012(9):21-27.

[39] 甄玉国.饲用微生态制剂--酵母培养物及其在反刍动物中的应用[J].饲料工业,2005,26(1):5-9.

[40] 詹谷宇,田萍,刘卫东,等.酵母菌生物合成谷胱甘肽[J].药学学报,1990,25(7):494-499.

[41] 张爱忠,卢德勋,姜宁,等.酵母培养物对绒山羊机体抗氧化能力的影响[J].动物营养学报,2010,22(3):781-786.

[42] 程元恺.谷胱甘肽的解毒作用与毒性代谢物[J].生物化学与生物物理进展,1994,21(5):395-399.

[43] 高姝娟,刘锡锰,高贵,等.谷胱甘肽的抗线粒体脂质过氧化作用[J].生物化学杂志,1997,13(3):287-290.

[44] 徐军发,黄迪南,吕世静,等.酵母多糖对体外培养小鼠腹腔巨噬细胞免疫功能的增强作用[J].广东医学院学报,2003,21(3):199-200.

[45] 朱延军,刘洪梅,边洪荣,等.酵母多糖对小鼠免疫功能影响的体外实验研究[J].安徽农业科学,2009(28):13636-13637.
Outlines

/