Effects of Vitamin C on Reproductive Performance and Offspring Quality of Tongue Sole (Cynoglossus semilaevis) Broodstocks

XIAO Dengyuan, LIANG Mengqing, ZHENG Keke, ZHAO Min

doi:10.3969/j.issn.1006-267x.2014.09.028

2014 , Vol. 26 >Issue 9: 2664 - 2674

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.09.028

Aquaculture Nutrition

Effects of Vitamin C on Reproductive Performance and Offspring Quality of Tongue Sole (Cynoglossus semilaevis) Broodstocks

Expand

1. Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
2. College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China

Received date: 2014-04-24

Online published: 2014-09-10

Fold

Abstract

This study was conducted to investigate the effects of vitamin C on reproduction performance and offspring quality of tongue sole (Cynoglossus semilaevis) broodstocks. Three experimental diets were prepared by adding 0.5% (C_0.5 group), 1.0% (C_1.0 group) and 1.5% (C_1.5 group) L-ascorbate-2-phosphate ( as vitamin C additive) into the basal diet, and the vitamin C supplemental levels in the 3 experimental diets were 0.175%, 0.350% and 0.525%, respectively. Fifty 3.5-year-old female broodstocks [(1.15±0.11) kg/fish] and twenty 3.5-year-old male broodstocks [(1.15±0.11) kg/fish] of tongue sole were fed one of three experimental diets for 2 months, and then artificial reproduction. The results showed that the supplementation of vitamin C had no significant effects on the gonadal index and relative fecundity of female broodstocks and the sperm concentration of male broodstocks (P>0.05), and the supplementation of 0.350% vitamin C could significantly increase the gonadal index of male broodstocks (P<0.05). With the vitamin C supplemental level increasing, buoyant egg ratio and hatching rate were significantly increased (P<0.05), while newly-hatched larval malformation ratio was significantly reduced (P<0.05). Egg diameter, fertilization rate, newly-hatched larval body length and the contents of estradiol and testosterone in serum of C_1.0 and C_1.5 groups were significantly higher than those of C_0.5 group (P<0.05), but no significant differences were observed between C_1.0 and C_1.5 groups (P>0.05). There was an increasing tendency in vitamin C content in egg and buoyant egg with the vitamin C supplemental level increasing, but no significant differences were observed among all experimental groups (P>0.05). Besides, the supplementation of vitamin C could increase the vitamin C content in liver, ovary, testicle and egg, enhance the superoxide dismutase activity in serum, liver, ovary, testicle, egg and seminal fluid, and reduce the malondialdehyde content, correspondingly. The results demonstrate that the high dose (0.525%) vitamin C supplemented in the diet of tongue sole broodstocks is advantageous to promote the synthesis of sex hormones, improve the reproductive performance of broodstocks and the quality of egg and sperm, promote the development of fertilized egg, and reduce the malformation of larvae under this experimental condition.

Key words： tongue sole (Cynoglossus semilaevis); broodstock; vitamin C; reproductive performance; offspring quality

Cite this article

XIAO Dengyuan, LIANG Mengqing, ZHENG Keke, ZHAO Min . Effects of Vitamin C on Reproductive Performance and Offspring Quality of Tongue Sole (Cynoglossus semilaevis) Broodstocks[J]. Chinese Journal of Animal Nutrition, 2014 , 26(9) : 2664 -2674 . DOI: 10.3969/j.issn.1006-267x.2014.09.028

References

[1] BENDICH A,MACHLIN L J,SCANDURRA O,et al.The antioxidant role of vitamin C[J].Advances in Free Radical Biology & Medicine,1986,2(2):419-444.

[2] DABROWSKI K,CIERESZKO A.Ascorbic acid and reproduction in fish:endocrine regulation and gamete quality[J].Aquaculture Research,2001,32(8):623-638.

[3] EMATA A C,BORLONGAN I G,DAMASO J P.Dietary vitamin C and E supplementation and reproduction of milkfish Chanos chanos Forsskal[J].Aquaculture Research,2000,31(7):557-564.

[4] BLOM J H,DABROWSKI K.Reproductive success of female rainbow trout (Oncorhynchus mykiss) in response to graded dietary ascorbyl monophosphate levels[J].Biology of Reproduction,1995,52(5):1073-1080.

[5] BLOMJ H,DABROWSKI K.Dietary ascorbyl phosphate results in high ascorbic acid content in eggs of rainbow trout[J].Comparative Biochemistry and Physiology Part A:Physiology,1995,112(1):75-79.

[6] FALAHATKAR B,DABROWSKI K,ARSLAN M.Ascorbic acid turnover in rainbow trout,Oncorhynchus mykiss:is there a vitamin enrichment effect during embryonic period on the juvenile fish "sensitivity" to deficiency?[J].Aquaculture,2011,320(1/2):99-105.

[7] LEE K J,DABROWSKI K.Long-term effects and interactions of dietary vitamins C and E on growth and reproduction of yellow perch,Perca flavescens[J].Aquaculture,2004,230(1/2/3/4):377-389.

[8] FURUITA H,ISHIDA T,SUZUKI T,et al.Vitamin content and quality of eggs produced by broodstock injected with vitamins C and E during artificial maturation in Japanese eel,Anguilla japonica[J].Aquaculture,2009,289(3/4):334-339.

[9] 李思忠,王惠民.中国动物志:硬骨鱼纲、鲽形目[M].北京:科学出版社,1995.

[10] 柳学周,庄志猛,马爱军,等.半滑舌鳎苗种生产技术的开发研究[J].海洋水产研究,2006,27(2):17-24.

[11] 刘振华,马峰,郑世竹.半滑舌鳎人工育苗技术研究[J].科学养鱼,2007(9):26-27.

[12] 朱丽华,王君霞.半滑舌鳎人工繁殖试验[J].河北渔业,2011(5):51,68.

[13] 柳学周,孙中之,马爱军,等.半滑舌鳎亲鱼培育及采卵技术研究[J].海洋水产研究,2006,27(2):25-32.

[14] 柳学周,庄志猛,马爱军,等.半滑舌鳎繁殖生物学及繁育技术研究[J].海洋水产研究,2005,26(5):7-14.

[15] 杨景峰,陈松林,翟介明,等.半滑舌鳎人工催产技术研究[J].内蒙古民族大学学报:自然科学版,2010,25(2):185-190.

[16] 徐永江,柳学周,马爱军,等.半滑舌鳎胚胎发育及仔鱼生长与盐度的关系[J].海洋科学,2005,29(11):39-43.

[17] AI Q H,MAI K S,TAN B P,et al.Effects of dietary vitamin C on survival,growth,and immunity of large yellow croaker,Pseudosciaena crocea[J].Aquaculture,2006,261(1):327-336.

[18] SOLIMAN A K,JAUNCEY K,ROBERTS R J.The effect of dietary ascorbic acid supplementation on hatchability,survival rate and fry performance in Oreochromis mossambicus (Peters)[J].Aquaculture,1986,59(3/4):197-208.

[19] SANDNES K,ULGENES Y,BRAEKKAN O R,et al.The effect of ascorbic acid supplementation in broodstock feed on reproduction of rainbow trout (Salmo gairdneri)[J].Aquaculture,1984,43(1/2/3):167-177.

[20] 张海涛,梁萌青,郑珂珂,等.饲料中维生素C对大菱鲆繁殖性能的影响[J].渔业科学进展,2013,34(2):73-81.

[21] DABROWSKI K,BLOMJ H.Ascorbic acid deposition in rainbow trout (Oncorhynchus mykiss) eggs and survival of embryos[J].Comparative Biochemistry and Physiology Part A: Physiology,1994,108(1):129-135.

[22] TEROVA G,SAROGLIA M,PAPP Z G,et al.Ascorbate dynamics in embryos and larvae of sea bass and sea bream,originating from broodstocks fed supplements of ascorbic acid[J].Aquaculture International,1998,6(5):357-367.

[23] TOLBERT B M,DOWNING M,CARLSON R W,et al.Chemistry and metabolism of ascorbic acid and ascorbate sulfate[J].Annals of the New York Academy of Sciences,1975,258:48-69.

[24] LEVINE M,MORITA K.Ascorbic acid in endocrine systems[J].Vitamins & Hormones,1985,42:1-64.

[25] WAAGBØ R,THORSEN T,SANDNES K.Role of dietary ascorbic acid in vitellogenesis in rainbow trout (Salmo gairdneri)[J].Aquaculture,1989,80(3/4):301-314.

[26] MOREAU R,DABROWSKI K.Body pool and synthesis of ascorbic acid in adult sea lamprey (Petromyzon marinus):an agnathan fish with gulonolactone oxidase activity[J].Proceedings of the National Academy of Sciences of the United States of America,1998,95(17):10279-10282.

[27] COWEY C B,BELL J G,KNOX D,et al.Lipids and lipid antioxidant systems in developing eggs of salmon (Salmo salar)[J].Lipids,1985,20(9):567-572.

[28] 唐黎,李辉,牟洪民,等.鱼类维生素C营养的研究概况[J].饲料工业,2011,32(12):56-60.

[29] CIERESZKO A,DABROWSKI K,LIN F,et al.Protective role of ascorbic acid against damage to male germ cells in rainbow trout (Oncorhynchus mykiss)[J].Canadian Journal of Fisheries and Aquatic Sciences,1999,56(2):178-183.

[30] 艾春香,陈立侨,周忠良,等.维生素C、E对中华绒螯蟹生殖性能的影响[J].水产学报,2003,27(1):64-68.

[31] BROMAGE N,JONES J,RANDALL C,et al.Broodstock management,fecundity,egg quality and the timing of egg production in the rainbow trout (Oncorhynchus mykiss)[J].Aquaculture,1992,100(1/2/3):141-166.

[32] BARNES M J,KODICEK E.Biological hydroxylations and ascorbic acid with special regard to collagen metabolism[J].Vitamins and Hormons,1972,30:1-43.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References