The experiment was conducted to investigate the effects of dietary cellulase on growth performance, digestion ability and non-specific immunity of juvenile sea cucumber (Apostichopus japonicus Selenka). Juvenile sea cucumbers with an average initial body weight of (1.44±0.01) g were fed with 6 experimental diets according to a single factor design: a basal diet supplemented with 0 (control), 0.025%, 0.050%, 0.100%, 0.200% and 0.400% cellulase for 49 days. There were 3 replicates per group with 35 sea cucumbers. The results showed as follows: supplementation of 0.025% to 0.100% cellulase could significantly enhance the specific growth rate (SGR) (P<0.05) and significantly reduce the feed conversion ratio (FCR) (P<0.05), and the highest SGR and lowest FCR of juvenile sea cucumbers were both found in 0.100% group. There were no significant differences in feed intake (FI) and survival rate (SR) among all groups (P>0.05). With the cellulase supplemental level increasing, the apparent digestibility coefficient of dry matter (ADCd) and the apparent digestibility coefficient of crude protein (ADCp) in diets were firstly raised and then declined, and the highest ADCd and ADCp were both found in 0.100% group which were significantly higher than those in control group (P<0.05). The ADCd in 0.400% group was not significantly different compared with control group (P>0.05), but the ADCp in 0.400% group was significantly lower than that in control group (P<0.05). The activity of amylase (AMS) in foregut, midgut and hindgut of juvenile sea cucumbers was increased by cellulase supplementation, and the supplementation of 0.100%, 0.200% and 0.400% cellulase had significant influences on AMS activity in foregut, midgut and hindgut, respectively (P<0.05). Supplementation of 0.100% to 0.200% cellulase could significantly increase the activities of superoxide dismutase (SOD) and acid phosphatase (ACP) in gut of juvenile sea cucumbers (P<0.05). It can be concluded that dietary cellulase can promote the growth, and enhance the digestion ability and non-specific immunity of juvenile sea cucumber in the condition of this experiment. It compromises the indexes on growth, digestion and non-specific immune, the optimal supplemental level of cellulase in the diet of juvenile sea cucumber is 0.100%.
QIN Bo, CHEN Siqing, CHANG Qing, LIU Changlin, LYU Yunyun, WANG Zhijun
. Effects of Dietary Cellulase on Growth Performance, Digestion Ability and Non-Specific Immunity of Juvenile Sea Cucumber (Apostichopus japonicus Selenka)[J]. Chinese Journal of Animal Nutrition, 2014
, 26(9)
: 2698
-2705
.
DOI: 10.3969/j.issn.1006-267x.2014.09.031
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