Effects of Low Protein Diet Supplemented Lysine and Methionine on Growth Performance and Serum Biochemical Indices of Weaner Sika Deer

HUANG Jian, BAO Kun, ZHANG Tietao, LI Guangyu, ZHOU Ning, WANG Kaiying, YANG Fuhe

doi:10.3969/j.issn.1006-267x.2014.09.033

2014 , Vol. 26 >Issue 9: 2714 - 2721

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.09.033

Special Economic Animal Nutrition

Effects of Low Protein Diet Supplemented Lysine and Methionine on Growth Performance and Serum Biochemical Indices of Weaner Sika Deer

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State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun 130112, China

Received date: 2014-03-19

Online published: 2014-09-10

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Abstract

This experiment was conducted to investigate the effects of low protein diet supplemented lysine (Lys) and methionine (Met) on growth performance and serum biochemical indices of weaner sika deer. Sixteen healthy 3-month-old male sika deer were randomly divided into 4 groups with 4 replicates per group and 1 sika deer per replicate. Restricted feeding 4 diets: control group (group Ⅰ) fed 16.28% crude protein (CP) based diet, and test groups fed 13.40 % CP diet supplemented with 0.23% Lys and 0 (group Ⅱ), 0.06% (group Ⅲ), 0.12% (group Ⅳ) Met, respectively. The results showed as follows: 1) the average daily gain (ADG) in group Ⅱ was the lowest, and the ADG in groups Ⅱ, Ⅲ and Ⅳ was increased with dietary Met level increased, the ADG in group Ⅳ was nearly the same as that in group Ⅰ(P>0.05). 2) The serum albumin (ALB) content on 56 days in group Ⅰ was significantly higher than that in groups Ⅱ and Ⅲ (P<0.05), the serum total protein (TP) content on 56 days was significantly higher than that in group Ⅱ (P<0.05), the serum urea nitrogen (UN) content on 28 days was significantly higher than that in group Ⅳ (P<0.01), the serum glucose (GLU) content on 56 days was significantly lower than that in groups Ⅱ and Ⅲ (P<0.01), the serum aspartic transaminase (AST) activity was significantly higher than that in group Ⅳ (P<0.05), the serum alkaline phosphatase (AKP) activity on 28 days was significantly higher than that in groups Ⅱ and Ⅲ (P<0.05 or P<0.01). 2) Compared among groups Ⅱ, Ⅲ and Ⅳ, the serum UN content on 28 days in group Ⅱ was significantly higher than that in group Ⅳ (P<0.05), the AKP activity in group Ⅳ on 28 days was significantly higher than that in groups Ⅱ and Ⅲ (P<0.05), but serum ALB and TP contents increased with Met level increased, serum AST and ALT activities and UN content decreased with Met level increased. In conclusion, the ADG of sika deer fed diet supplemented Lys and Met with a CP level of 13.40% is the same as that fed diet with a CP level of 16.28%. The supplemented 0.12% Met in diets can enhance growth and metabolism balance of sika deer.

Key words： sika deer; low protein; lysine; methionine; growth performance; serum biochemical indices

Cite this article

HUANG Jian, BAO Kun, ZHANG Tietao, LI Guangyu, ZHOU Ning, WANG Kaiying, YANG Fuhe . Effects of Low Protein Diet Supplemented Lysine and Methionine on Growth Performance and Serum Biochemical Indices of Weaner Sika Deer[J]. Chinese Journal of Animal Nutrition, 2014 , 26(9) : 2714 -2721 . DOI: 10.3969/j.issn.1006-267x.2014.09.033

References

[1] NOLTE J V E,LOEST C A,FERREIRA A V,et al.Limiting amino acids for growing lambs fed a diet low in ruminally undegradable protein[J].Journal of Animal Science,2008,86(10):2627-2641.

[2] ABE M,IRIKI T,FUNABA M.Lysine deficiency in postweaned calves fed corn and corn gluten meal diets[J].Journal of Animal Science,1997,75(7):1974-1982.

[3] 张乃峰.蛋白质与氨基酸营养对早期断奶犊牛免疫相关指标的影响[D].博士学位论文.北京:中国农业科学院,2008.

[4] 王建红,刁其玉,许先查,等.赖氨酸、蛋氨酸和苏氨酸对犊牛生长性能和血清生化指标的影响[J].动物营养学报,2011,23(2):226-233.

[5] BRODERICK G A,STEVENSON M J,PATTON R A,et al.Effect of dietary protein concentration and degradability on response to rumen-protected methionine in lactating dairy cows[J].Journal of Dairy Science,2009,92(6):2719-2728.

[6] LEE C,HRISTOV A N,CASSIDY T W,et al.Rumen-protected lysine,methionine,and histidine increase milk protein yield in dairy cows fed a metabolizable protein-deficient diet[J].Journal of Dairy Science,2012,95(10):6042-6056.

[7] 云强,刁其玉,屠焰,等.日粮中赖氨酸和蛋氨酸比对断奶犊牛生长性能和消化代谢的影响[J].中国农业科学,2011,44(1):133-142.

[8] PIEPENBRINK M S,OVERTON T R,CLARK J H.Response of cows fed a low crude protein diet to ruminally protected methionine and lysine[J].Journal of Dairy Science,1996,79(9):1638-1646.

[9] BARTLETT K S,MCKEITH F K,VANDEHAAR M J,et al.Growth and body composition of dairy calves fed milk replacers containing different amounts of protein at two feeding rates[J].Journal of Animal Science,2006,84 (6):1454-1467.

[10] KLEMESRUD M J,KLOPFENSTEIN T J,LEWIS A J.Metabolize methionine and lysine requirements of growing cattle[J].Journal of Animal Science,2000,78(1):199-206.

[11] FIGUEROA J L,LEWIS A J,MILLER P S.Nitrogen metabolism and growth performance of gilts fed standard corn-soybean meal diets or low-crude protein,amino acid-supplemented diets[J].Journal of Animal Science,2002,80(11):2911-2919.

[12] 李辉,刁其玉,张乃锋,等.不同蛋白水平对犊牛消化代谢及血清生化指标的影响[J].中国农业科学,2008,41(4):1219-1226.

[13] 李辉,刁其玉,张乃锋,等.不同蛋白质来源对早期断奶犊牛消化及血清生化指标的影响(一)[J].动物营养学报,2009,21(1):47-52.

[14] 余红心,贾俊静,李琦华,等.不同蛋白质水平日粮对云南武定鸡生长性能及血液生化指标的影响[J].中国饲料,2009(5):24-26.

[15] 王建红,刁其玉,许先查,等.日粮Lys、Met和Thr添加模式对0-2月龄犊牛生长性能、消化代谢与血清学生化指标的影响[J].中国农业科学,2011,44(9):1898-1907.

[16] 侯生珍,吕凯,王志有,等.蛋白质水平及Lys/Met对早期断奶藏羔羊应激和免疫的影响[J].中国畜牧杂志,2013(15):65-69.

[17] COFFEY M T,HAGLER W M,CULLEN J M.Influence of dietary protein,fat or amino acids on the response of weanling swine to aflatoxin B1[J].Journal of Animal Science,1989,67(2):465-472.

[18] 刘文斐,刘伟龙,占秀安,等.不同形式蛋氨酸对肉种鸡生产性能、免疫指标及抗氧化功能的影响[J].动物营养学报,2013,25(9):2118-2125.

[19] 鲍坤,李光玉,崔学哲,等.不同形式铜对雄性梅花鹿血清生化指标及营养物质消化率的影响(英文)[J].动物营养学报,2010,22(3):717-722.

[20] 赵欣平,张久源,杨守忠,等.白蜡虫碱性磷酸酶功能基团的研究[J].昆虫学报,2001,44(3):257-262.

[21] 唐靓婷.牛肝碱性磷酸酶的分离纯化、部分性质和功能基团及固定化研究[D].硕士学位论文.重庆:西南大学,2010.

[22] 张勇,韩春晓,朱宇旌,等.饲粮中添加包被蛋氨酸对肉鸡生长性能、血液生化指标和养分表观回肠消化率的影响[J].动物营养学报,2012,24(4):739-746.

[23] 刘凤华,孙伟丽,钟伟,等.饲粮蛋白质水平对银黑狐生长性能及氮代谢的影响[J].动物营养学报,2011,23(11):2024-2030.

[24] COMA J,CARRION D,ZIMMERMAN D R.Use of plasma urea nitrogen as a rapid response criterion to determin the lysine requirement of pigs[J].Journal of Animal Science,1995,73(2):472-481.

[25] 王洪荣,卢德勋.饲喂玉米型日粮的生长绵羊限制性氨基酸研究[J].动物营养学报,1999,11(4):17-28.

[26] SCHROEDER G F,TITGEMEYER E C,AWAWDEH M S,et al.Effects of energy source on methionine utilization by growing steers[J].Journal of Animal Science,2006,84 (6):1505-1511.

[27] DANIELS K M,HILL S R,KNOWLTON K F,et al.Effects of milk replacer composition on selected blood metabolites and hormones in preweaned holstein heifers[J].Journal of Dairy Science,2008,91(7):2628-2640.

[28] YANG S Y,OH Y K,AHN H S,et al.Maintenance crude protein requirement of penned female Korean spotted deer (Cervus nippon)[J].Asian-Australasian Journal of Animal Science,2014,27(1):30-35.

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