Fatty Acid Transport Protein 1: Expression Pattern in Different Tissues of Rongchang Pigs and Correlation with Intramuscular Fat Deposition

QI Renli, CHEN Ying, PENG Han, LIU Yan, HUANG Jinxiu, YANG Feiyun

doi:10.3969/j.issn.1006-267x.2014.09.035

2014 , Vol. 26 >Issue 9: 2730 - 2735

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.09.035

Molecular Nutrition

Fatty Acid Transport Protein 1: Expression Pattern in Different Tissues of Rongchang Pigs and Correlation with Intramuscular Fat Deposition

Expand

Chongqing Academy of Animal Sciences, Chongqing Key Laboratory of Pig Industry Sciences, Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Rongchang 402460, China

Received date: 2014-04-03

Online published: 2014-09-10

Fold

Abstract

The objective of this experiment is to study the expression pattern of fatty acid transport protein 1 (FATP1) gene in different tissues of Rongchang pigs and further to assay the correlation between FATP1 expression level and intramuscular fat deposition in the pigs. A total of 80 healthy 1-day-old Rongchang piglets were fed and 5 of them were slaughtered at 1, 7, 20, 50, 90 and 160 days of age, respectively. The mRNA and protein level of FATP1 were detected in the pigs. Moreover, its correlation with intramuscular fat deposition was assayed. The results showed that the FATP1 gene and coded protein were widely expressed in various tissues of Rongchang pigs. The muscle and heart had the highest expression levels and the expression levels in the adipose tissue and liver were medium, while the small intestine and lung had the lowest expression levels. With the growth of piglets, the expression level of FATP1 gene in muscle increased gradually and up to a higher level after 50 days of age in the longissimus muscle and after 90 days of age in the semitendinosus muscle, respectively. Data of analysis showed that there was a significant positive correlation between the expression of FATP1 gene and intramuscular fat deposition (P<0.05). It is concludes that the higher expression levels of FATP1 gene and its protein have an important influence on the porcine intramuscular fat formation and deposition.

Key words： FATP1; intramuscular fat; gene expression; pig

Cite this article

QI Renli, CHEN Ying, PENG Han, LIU Yan, HUANG Jinxiu, YANG Feiyun . Fatty Acid Transport Protein 1: Expression Pattern in Different Tissues of Rongchang Pigs and Correlation with Intramuscular Fat Deposition[J]. Chinese Journal of Animal Nutrition, 2014 , 26(9) : 2730 -2735 . DOI: 10.3969/j.issn.1006-267x.2014.09.035

References

[1] SCHAAP F G,HAMERS L,VAN DER VUSSE G J,et al.Molecular cloning of fatty acid-transport protein cDNA from rat[J].Biochimica et Biophysica Acta,1997,1354(1):29-34.

[2] DOEGE H,STAHL A.Protein-mediated fatty acid uptake:novel insights from in vivo models[J].Physiology,2006,21:259-268.

[3] STAHL A.A current review of fatty acid transport proteins (SLC27)[J].Pflügers Archiv,2004,447(5):722-727.

[4] HARASIM E,KALINOWSKA A,CHABOWSKI A,et al.The role of fatty-acid transport proteins (FAT/CD36,FABPpm,FATP) in lipid metabolism in skeletal muscles[J].Postepy Higieny i Medycyny Doswiadczalnej,2008,62:433-441.

[5] BERGER J,TRUPPE C,NEUMANN H,et al.A novel relative of the very long-chain acyl-CoA synthetase and fatty acid transporter protein genes with a distinct expression pattern[J].Biochemical and Biophysical Research Communications,1998,247(2):255-260.

[6] WATKINS P A,PEVSNER J,STEINBERG S J.Human very long-chain acyl-CoA synthetase and two human homologs:initial characterization and relationship to fatty acid transport protein[J].Prostaglandins,Leukotrienes and Essential Fatty Acids,1999,60(5/6):323-328.

[7] WANG Y,ZHU Q,ZHAO X L,et al.Age-related expression profile of the SLC27A1 gene in chicken tissues[J].Molecular Biology Reports,2011,38(8):5139-5145.

[8] GALLARDO D,AMILLS M,QUINTANILLA R,et al.Mapping and tissue mRNA expression analysis of the pig solute carrier 27A (SLC27A) multigene family[J].Gene,2013,515(1):220-223.

[9] SONG Y Z,FENG J Y,ZHOU L H,et al.Molecular cloning and ontogenesis expression of fatty acid transport protein-1 in yellow-feathered broilers[J].Journal of Genetics and Genomics,2008,35(6):327-333.

[10] 张海燕,姜学,尹靖东.猪肌内脂肪沉积的营养调控及候选基因的研究进展[J].中国畜牧杂志,2009,45(21):60-64.

[11] 齐仁立,黄金秀,杨飞云,等.脂肪酸转运蛋白家族及其介导的脂肪酸跨膜转运[J].动物营养学报,2013,25(5):905-911.

[12] POHL J,RING A,KORKMAZV,et al.FAT/CD 36-mediated long-chain fatty acid uptake in adipocytes requires plasma membrane rafts[J].Molecular Biology of the Cell,2005,16(1):24-31.

[13] NICKERSON J G,ALKHATEEB H,BENTON C R,et al.Greater transport efficiencies of the membrane fatty acid transporters fat/cd36 and fatp4 compared with fabppm and fatp1 and differential effects on fatty acid esterification and oxidation in rat skeletal muscle[J].The Journal of Biological Chemistry,2009,284(24):16522-16530.

[14] 王彦.鸡FATP1/FATP4基因的克隆、表达及其与屠体性状的关联分析[D].博士学位论文.雅安:四川农业大学,2009:8-21.

[15] 罗轶.鸡FATP1基因cDNA的克隆、组织表达及其生物信息学分析[D].硕士学位论文.雅安:四川农业大学,2008:12-30.

[16] 华绪川,张立凡,蔡兆伟,等.猪FATP1基因5'调控区多态性及其与脂肪性状的相关分析[J].江苏农业学报,2011,27(1):89-93.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References