Effects of Bta-MicroRNA-145 on IGF1R-PI3K-Akt/mTOR Signal Pathway Related Gene Expressions and Reveal of Its Potential Target

LI Wenqing, WANG Jiaqi, NAN Xuemei, BU Dengpan

doi:10.3969/j.issn.1006-267x.2014.09.036

2014 , Vol. 26 >Issue 9: 2736 - 2744

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.09.036

Molecular Nutrition

Effects of Bta-MicroRNA-145 on IGF1R-PI3K-Akt/mTOR Signal Pathway Related Gene Expressions and Reveal of Its Potential Target

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1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Life Science, Henan Agricultural University, Zhengzhou 450002, China;
3. CAAS-ICRAF Joint Laboratory on Agroforestry and Sustainable Animal Husbandr, Beijing 100193, China;
4. Synergetic Innovation Center of Safety and Nutrition, Northeast Agricultural University, Harbin 150030, China

Received date: 2014-04-21

Online published: 2014-09-10

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Abstract

The object of this experiment was to study the effects of bta-microRNA-145 (bta-miR-145) on the expressions of genes related to insulin-like growth factor 1 receptor-phosphatidylinositol 3-kinase-protein kinase B-mammalian target of rapamycin (IGF1R-PI3K-Akt/mTOR) signaling pathway, and to provide the basis for the regulation of lactation in dairy cow from the perspective of the microRNA. Primary bovine mammary epithelial cells were selected as research object. bta-miR-145 mimic and inhibitor were transfected into cells. After transfection, the expression changes of 15 genes related to IGF1R-PI3K-Akt/mTOR signaling pathway were detected by real-time qPCR. The results showed that the over-expression or down-expression of bta-miR-145 did not cause the expression change of predicted target IGF1R, insulin receptor substrate 1 (IRS1) and beta-casein (CSN2) (P>0.05); the over-expression of bta-miR-145 significantly caused down-regulation of eukaryotic translation initiation factor 4E (EIF4E) mRNA expression (P<0.05), and the down-expression of bta-miR-145 significantly caused increase of EIF4E mRNA expression (P<0.05), and bioinformatics analysis showed that the possible binding site of bovine EIF4E 3'-untranslated region (3'UTR) for bta-miR-145 existed. It is concluded that bta-miR-145 has a role in regulation of IGF1R-PI3K-Akt/mTOR signaling pathway, and EIF4E is a potential target of bta-miR-145.

Key words： bovine mammary epithelial cells; bta-miR-145; over-expression; down-expression; signal pathway; EIF4E

Cite this article

LI Wenqing, WANG Jiaqi, NAN Xuemei, BU Dengpan . Effects of Bta-MicroRNA-145 on IGF1R-PI3K-Akt/mTOR Signal Pathway Related Gene Expressions and Reveal of Its Potential Target[J]. Chinese Journal of Animal Nutrition, 2014 , 26(9) : 2736 -2744 . DOI: 10.3969/j.issn.1006-267x.2014.09.036

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