Feed Science and Technology

Tert-Butylhydroquinone: Preventive Effects on Heat Stress-Induced Oxidative Damage in Liver of Mice

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  • College of Animal Science and Technology, Nanjing Agriculture University, Nanjing 210095, China

Received date: 2014-03-01

  Online published: 2014-09-10

Abstract

To investigate the effects of tert-butylhydroquinone (tBHQ) on heat stress-induced oxidative damage in liver of mice, fifty six-week-old ICR male mice were divided into 2 groups with 25 mice in each group. tBHQ (-) group received a basal diet, and tBHQ (+) group received the basal diet supplemented with 1% tBHQ. After 14 days of feeding, mice were exposed to the elevated ambient temperature (42℃) daily for 2 h. During the period of 8 consecutive days, five mice in each group were sacrificed to collect liver before heat stress (0 day after heat stress) and at 1, 2, 4 and 8 days after heat stress, respectively. Liver weight and antioxidative indices were determined, transcription factor E2 related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) protein expressions in liver were determined by immunohistochemical detection, and the expressions of Nrf2 gene and Nrf2-regulated genes were analyzed by real time-PCR detection. The results showed as follows: 1) liver index of tBHQ (-) group recovered to normal level at 4 days after heat stress, while that of tBHQ (+) group recovered to normal level at 2 days after heat stress. 2) Compared with tBHQ (-) group, total superoxide dismutase (T-SOD) activity of tBHQ (+) group was significantly increased at 0 day after heat stress (P<0.05), and reduced glutathione hormone (GSH) content and the activities of T-SOD, copper, zinc-superoxide dismutase (Cu, Zn-SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) of tBHQ (+) group were significantly reduced at 4 days after heat stress (P<0.05). 3) At 2 days after heat stress, liver injury of tBHQ (-) group was more serious than that of tBHQ (+) group. 4) Nrf2 protein expression was not detected in liver at 0 day after heat stress, but it was detected at all the time points after heat stress of tBHQ (-) group and was detected at all the time points of tBHQ (+) group; HO-1 protein expression was detected in liver at 0, 1, 4 and 8 days after heat stress of tBHQ (-) group, and at 0, 1 and 2 days after heat stress of tBHQ (+) group. 5) Compared with tBHQ (-) group, multiple genes of kelch-like ECH-associated protein 1 (Keap1)-Nrf2/ARE signing pathway of tBHQ (+) group were significantly increased (P<0.05). In conclusion, dietary supplementation of 1% tBHQ can alleviate heat stress-induced oxidative damage in liver of mice.

Cite this article

ZHOU Xin, HUANG Yi, LI Yansen, LI Chunmei . Tert-Butylhydroquinone: Preventive Effects on Heat Stress-Induced Oxidative Damage in Liver of Mice[J]. Chinese Journal of Animal Nutrition, 2014 , 26(9) : 2779 -2788 . DOI: 10.3969/j.issn.1006-267x.2014.09.041

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