Regional and Ontogenetic Expression of PepT1,B0AT and EAAT3 mRNA in Intestine of Arbor Acre Broilers

ZHANG Aihua, ZHANG Shu, CAI Huiyi, LIU Guohua, CHANG Wenhuan, YAN Haijie, LI Tingting

doi:10.3969/j.issn.1006-267x.2014.09.048

Chinese Journal of Animal Nutrition >

2014 , Vol. 26 >Issue 9: 2841 - 2850

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.09.048

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Regional and Ontogenetic Expression of PepT1,B⁰AT and EAAT3 mRNA in Intestine of Arbor Acre Broilers

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1. Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2. National Engineering Research Center of Biological Feed, Beijing 100081, China

Received date: 2014-03-19

Online published: 2014-09-10

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Abstract

The objective of this study was to investigate the regional and ontogenetic expression of peptide transporter 1 (PepT1), system B⁰ neutral amino-acid transporter (B⁰AT) and excitatory amino-acid transporter 3 (EAAT3) mRNA in intestine of arbor acre (AA) broilers. A total of 120 one-day-old AA male broilers were randomly designed into 8 groups, selected 1 broiler from each group at 1, 3, 7, 14, 21, 28, 35 and 42 days of age, and intestinal tissues were collected and separated into duodenum, jejunum, and ileum. The expression of PepT1, B⁰AT, and EAAT3 mRNA were determined using real-time fluorescence quantitative PCR. The results showed as follows: 1) the relative expression of PepT1, B⁰AT and EAAT3 mRNA had significantly differences in different intestinal segments of broilers (P<0.05). The PepT1 mRNA relative expression was the highest in duodenum, the lowest in ileum and intermediate in jejunum, whereas the B⁰AT and EAAT3 mRNA relative expressions were the highest in ileum, the lowest in duodenum and intermediate in jejunum. 2) The PepT1, B⁰AT and EAAT3 mRNA relative expression had significantly differences in different day of age of broilers (P<0.01), while PepT1, B⁰AT and EAAT3 mRNA relative expression increased with the increase of day of age in early growth stage, then maintain the trend of stability in late stage. The study results indicate that peptide and amino acid transporters-PepT1, B⁰AT and EAAT3 mRNA expressions are significantly influenced by intestinal segment and day of age.

Key words： broilers; intestinal; PepT1; amino acid transporter; ontogenetic changes

Cite this article

ZHANG Aihua, ZHANG Shu, CAI Huiyi, LIU Guohua, CHANG Wenhuan, YAN Haijie, LI Tingting . Regional and Ontogenetic Expression of PepT1,B⁰AT and EAAT3 mRNA in Intestine of Arbor Acre Broilers[J]. Chinese Journal of Animal Nutrition, 2014 , 26(9) : 2841 -2850 . DOI: 10.3969/j.issn.1006-267x.2014.09.048

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