Cytosolic Phospholipase A2 Gene: Cloning and Expression Level Change with Growth Development of Large Yellow Croaker (Larmichthys crocea) Larvae

FENG Shuoheng, XIE Fengjun, CAI Zuonan, MAI Kangsen, AI Qinghui

doi:10.3969/j.issn.1006-267x.2014.09.053

2014 , Vol. 26 >Issue 9: 2883 - 2891

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.09.053

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Cytosolic Phospholipase A₂ Gene: Cloning and Expression Level Change with Growth Development of Large Yellow Croaker (Larmichthys crocea) Larvae

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Key Laboratory of Mariculture of Ministry of Education, Key Laboratory of Aquaculture Nutrition and Feed of Ministry of Agriculture, Ocean University of China, Qingdao 266003, China

Received date: 2014-04-14

Online published: 2014-09-10

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Abstract

In this present study, the full length of cytosolic phospholipase A₂ (cPLA₂) cDNA from large yellow croaker (Larmichthys crocea) was obtained by homology-based cloning and rapid amplification of cDNA ends (RACE) techniques. And, the expression level of cPLA₂ gene of large yellow croaker larvae at different days of age was measured by real-time quantitative PCR method. Sequence analysis results showed that the full-length of cPLA₂ cDNA (GenBank accession No.: KF006240.1) was 2 660 bp, which consists of 2 169 bp in size with an open-reading frame (ORF) encoding 723 amino acids, a 176 bp 5'-untranslated region (5'UTR) and a 205 bp 3'-untranslated region (3'UTR). Phylogenetic tree analysis results showed that the cPLA₂ gene of large yellow croaker had a closer relationship with other fish species, but had a distant relationship with mammalian. Quantitative determination results showed that with the days of age of large yellow croaker increasing, the expression level of cPLA₂ gene was significantly increased at first (1, 3 and 7 days of age vs. 15 days of age, P<0.05) and the 15 days of age had the highest value, then significantly decreased (15 days of age vs. 19 days of age, P<0.05), maintained stable until the end of the experiment. In conclusion, the key enzyme of phospholipid catabolism-cPLA₂ gene expression level show a regular variation during the deformation process from larvae to juvenile of large yellow croaker, which may has an important significance for keeping the dynamic equilibrium of phospholipids and maintaining the fluidity of membrane. The change trend of cPLA₂ gene expression level of large yellow croaker larvae can estimate the development of digestive system of large yellow croaker in a certain degree.

Key words： large yellow croaker; cytosolic phospholipase A₂; cloning; phospholipids; catabolism; growth development

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FENG Shuoheng, XIE Fengjun, CAI Zuonan, MAI Kangsen, AI Qinghui . Cytosolic Phospholipase A₂ Gene: Cloning and Expression Level Change with Growth Development of Large Yellow Croaker (Larmichthys crocea) Larvae[J]. Chinese Journal of Animal Nutrition, 2014 , 26(9) : 2883 -2891 . DOI: 10.3969/j.issn.1006-267x.2014.09.053

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