Effects of Lipopolysaccharide Challenge on Key Gene Expression in Toll-Like Receptor 4 Signaling Pathway of Hypothalamic-Pituitary-Adrenal Axis of Piglets

CHEN Shaokui, LIU Yulan, LI Quan, ZHU Huiling, HOU Yongqing, WU Huanting, WANG Xiuying, LIU Chen

doi:10.3969/j.issn.1006-267x.2014.11.023

2014 , Vol. 26 >Issue 11: 3356 - 3361

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.11.023

Molecular Nutrition

Effects of Lipopolysaccharide Challenge on Key Gene Expression in Toll-Like Receptor 4 Signaling Pathway of Hypothalamic-Pituitary-Adrenal Axis of Piglets

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Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China

Received date: 2014-05-05

Online published: 2014-11-17

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Abstract

This experiment was conducted to investigate the effects of lipopolysaccharide (LPS) challenge on the expression of the key genes in toll-like receptor 4 (TLR4) signaling pathway of hypothalamic-pituitary-adrenal (HPA) axis of piglets. Twelve weaner piglets were allotted to two groups (6 replicates per group and 1 piglet per replicate). The piglets in the experimental group were injected with 100 μg/kg body weight LPS, whereas the piglets in the control group were injected with an equivalent amount of sterile saline. At 4 h post-challenge, blood samples were collected for determining stress-related hormones contents in the plasma. Following blood collection at 4 h, the piglets were slaughtered, and hypothalamus, pituitary gland and adrenal gland were collected. The mRNA expression levels of the key genes in TLR4 signaling pathway, including TLR4, myeloid differentiation factor 88 (MyD88), IL-1 receptor-associated kinase 1 (IRAK1), tumor necrosis factor receptor-associated factor 6 (TRAF6) and nuclear factor-κB (NF-κB) in HPA axis, were detected by real-time quantitative PCR. The results showed as follows: compared with control group, 1) LPS significantly increased plasma TNF-α, cortisol and adrenocorticotropin contents (P<0.05). 2)After LPS challenge, TLR4 mRNA expression level was significantly increased in hypothalamus, pituitary gland and adrenal gland (P<0.05). MyD88 mRNA expression level was significantly increased in pituitary gland and adrenal gland (P<0.05). The mRNA expression level of MyD88 in the hypothalamus had a tendency to increase (P<0.10). IRAK1 mRNA expression level in pituitary gland and adrenal gland had a tendency to increase (P<0.10). TRAF6 mRNA expression level was significantly increased in hypothalamus, pituitary gland and adrenal gland (P<0.05). NF-κB mRNA expression level was significantly increased in pituitary gland (P<0.05). These results indicate that LPS challenge actives HPA axis and induces the mRNA expression levels of the key genes in TLR4 signaling pathway of HPA axis.

Key words： piglets; LPS; HPA axis; TLR4 signaling pathway

Cite this article

CHEN Shaokui, LIU Yulan, LI Quan, ZHU Huiling, HOU Yongqing, WU Huanting, WANG Xiuying, LIU Chen . Effects of Lipopolysaccharide Challenge on Key Gene Expression in Toll-Like Receptor 4 Signaling Pathway of Hypothalamic-Pituitary-Adrenal Axis of Piglets[J]. Chinese Journal of Animal Nutrition, 2014 , 26(11) : 3356 -3361 . DOI: 10.3969/j.issn.1006-267x.2014.11.023

References

[1] GABLER N K,SPURLOCK M E.Integrating the immune system with the regulation of growth and efficiency[J].Journal of Animal Science,2008,86(Suppl.14):E64-E74.

[2] SABROE I,PARKER L C,DOWER S K,et al.The role of TLR activation in inflammation[J].The Journal of Pathology,2008,214(2):126-135.

[3] HADDAD J J.Oxygen-sensitive pro-inflamnatory cytokines,apoptosis signaling and redox-responsive transcription factors in development and pathophysiology[J].Cytokines Cellular and Molecular Therapy,2002,7(1):1-14.

[4] 汪军.电针对创伤应激大鼠外周和中枢TLR2/4以及炎性细胞因子表达的调节作用与机制[D].博士学位论文.上海:复旦大学,2009.

[5] 陈逢,刘玉兰,李权,等.TLR4信号通路关键基因在断奶仔猪不同组织中的mRNA表达[J].中国畜牧杂志,2013,49(17):53-57.

[6] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCt method[J].Methods,2001,25(4):402-408.

[7] JOHNSON R W.Inhibition of growth by pro-inflammatory cytokines:an integrated view[J].Journal Animal Science,1997,75(5):1244-1255.

[8] S?OTWI ?SKI R,S?OTWI#323; SKA S,KEDZIORA S,et al.Innate immunity signaling pathways:links between immunonutrition and responses to sepsis[J].Archivum Immunologiae et Therapiae Experimentalis,2011,59(2):139-150.

[9] 陈洁,姜虹.TLR4信号通路与炎性反应[J].医学综述,2009,15(19):2902-2904.

[10] LEE J Y,YE J,GAO Z,et al.Reciprocal modulation of Toll-like receptor-4 signaling pathways involving MyD88 and phosphatidylinositol 3-kinase/AKT by saturated and polyunsaturated fatty acids[J].The Journal of Biological Chemistry,2003,278(39):37041-37051.

[11] LIU Y L,CHEN F,ODLE J,et al.Fish oil increases muscle protein mass and modulates Akt/FOXO,TLR4 and NOD signaling in weaning piglets after lipopolysaccharide challenge[J].The Journal of Nutrition,2013,143(8):1331-1339.

[12] LIU Y L,CHEN F,ODLE J,et al.Fish oil enhances intestinal integrity and inhibits TLR4 and NOD2 signaling pathways in weaned pigs after LPS challenge[J].The Journal of Nutrition,2012,142(11):2017-2024.

[13] CHEN F,LIU Y L,ZHU H L,et al.Fish oil attenuates liver injury caused by LPS in weaned pigs associated with inhibition of TLR4 and nucleotide-binding oligomerization domain protein signaling pathways[J].Innate Immunity,2013,19(5):504-515.

[14] 肖品品,耿直.癫痫持续状态大鼠海马组织中NR1和TLR4的表达及其相关性[J].中风和神经疾病杂志,2013,30(12):1077-1079.

[15] 王意.TLR4信号通路在老龄大鼠术后认知功能下降中的作用研究[D].博士学位论文.长沙:中南大学,2013.

[16] 高音.TLR4介导的MyD88信号通路在小鼠脑缺血再灌注损伤中的作用[D].博士学位论文.沈阳:中国医科大学,2009.

[17] KANCZKOWSKI W,ALEXAKI V I,TRAN N,et al.Hypothalamo-pituitary and immune-dependent adrenal regulation during systemic inflammation[J].Proceedings of the National Academy of Sciences of the United States of America,2013,110(36):14801-14806.

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