Effects of Cecropin A-Magainin Hybrid Peptide on Small Intestinal Mucosal Structure, Mucosal Immune Function and Intestinal Microflora in Mice

YANG Tiantian, YU Longmei, LIU Erqiang, CHEN Xiangjun, ZHU Mingxing, WANG Xiuqing

doi:10.3969/j.issn.1006-267x.2014.11.027

2014 , Vol. 26 >Issue 11: 3387 - 3395

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.11.027

Feed Science and Technology

Effects of Cecropin A-Magainin Hybrid Peptide on Small Intestinal Mucosal Structure, Mucosal Immune Function and Intestinal Microflora in Mice

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1. School of Clinical Laboratory of Medicine of Ningxia Medical University, Yinchuan 750004, China;
2. Science and Technology Center of Ningxia Medical University, Yinchuan 750004, China

Received date: 2014-05-03

Online published: 2014-11-17

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Abstract

This experiment was to study the effects of cecropin A-magainin hybrid peptide on small intestinal mucosal structure, mucosal immune function and intestinal microflora in mice. The eighteen healthy BALB/c mice with similar weight were randomly divided into three groups: control group (given 0.75 mL saline water by gastric lavage), low dose of hybrid peptide group (given 0.75 mL 0.26 mg/mL cecA-mag peptide by gastric lavage), and high dose of hybrid peptide group (given 0.75 mL 0.52 mg/mL cecA-mag peptide by gastric lavage). The feeding experiment lasted for 6 weeks. The results showed as follows: 1) the villous length of duodenum in two cecA-mag peptide groups was higher than that in control group (P<0.05); the crypt depth of every part of small intestinal in two cecA-mag peptide groups was significantly lower than that in control group (P<0.05); and villous length/crypt depth (V/C) of every part of small intestinal was significantly higher than that in control group (P<0.05). 2) Compared with control group, the positive expression levels of IgA in duodenum, jejunum, and ileum were significantly higher in two cecA-mag peptide groups (P<0.05). 3) The contents of IL-2, IFN-γ and IL-4 in two cecA-mag peptide groups were significantly higher than those in control group (P<0.05). IFN-γ/IL-4 was not significantly different among different groups (P>0.05). 4) The number of Escherichia coli in two cecA-mag peptide groups was significantly lower than that in control group (P<0.05), the number of Bifidobacterium and Lactobacillus in two cecA-mag peptide groups was significantly higher than that in control group (P<0.05). It is concluded that cecropin A-magainin hybrid peptide can improve mucosal structure of small intestine and may enhance intestinal mucosal immune function through enhancing the expression of IgA, improve cellular immunity through enhancing the secreting of IL-2 and IFN-γ, improve humoral immunity through enhancing the secreting of IL-4, keep the balance of Th1/Th2, effectsively decrease the number of Escherichia coli of pathogenic bacteria and increase the number of Bifidobacterium and Lactobacillus of intestinal probiotics.

Key words： cecropin A-magainin hybrid peptide; mice; small intestinal mucosal structure; mucosal immunity; cytokine; intestinal microflora

Cite this article

YANG Tiantian, YU Longmei, LIU Erqiang, CHEN Xiangjun, ZHU Mingxing, WANG Xiuqing . Effects of Cecropin A-Magainin Hybrid Peptide on Small Intestinal Mucosal Structure, Mucosal Immune Function and Intestinal Microflora in Mice[J]. Chinese Journal of Animal Nutrition, 2014 , 26(11) : 3387 -3395 . DOI: 10.3969/j.issn.1006-267x.2014.11.027

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