Feed Science and Technology

Impact of N-Acetylcysteine on Antioxidant Ability and Intestinal Microflora in Aging Mice

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  • Shanghai Key Laboratory for Veterinary and Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China

Received date: 2014-05-09

  Online published: 2014-11-17

Abstract

The aim of this study was to investigate the effects of N-acetylcysteine (NAC) on antioxidant ability and intestinal microflora in aging mice. D-galactose induced aging mice models were designed firstly. NAC containing water was provided for these mice freely. The activities of glutathione peroxidase (GSH-Px) and catalase (CAT) and malonaldehyde (MDA) content were subsequently measured in the ileum tissues. Finally, changes in intestinal flora were observed by denaturing gradient gel electrophoresis (DGGE), and fluorescence quantitative RT-PCR was used to measure the mRNA expression of adhesion associated genes in the ileum of mice. Results showed as follows: after NAC supplementation, the activities of GSH-Px and CAT were significantly increased (P<0.05), while the content of MDA was significantly reduced in the ileum of aging mice (P<0.05). Compared with the NAC untreated aging mice, NAC could increase the number of Lactobacillus, Bifidobacterium, and Enterococcus and decrease the number of E. coli in intestinal tract of aging mice. After NAC treatment, the gene expressions of trefoil factor family 3 (TFF3) and mucin 3 (MUC3) were rapidly increased in the ileum of aging mice. Therefor, NAC has the potential to regulate the intestinal microflora changes via the antioxidant capacity regulation. The regulation mechanism of intestinal microflora in aging mice might be associated with the gene expression changes in TFF3 and MUC3.

Cite this article

BAO Jian, CAI Xuan, SHAO Liyu, SHENG Yongshuai, XU Jianxiong . Impact of N-Acetylcysteine on Antioxidant Ability and Intestinal Microflora in Aging Mice[J]. Chinese Journal of Animal Nutrition, 2014 , 26(11) : 3396 -3403 . DOI: 10.3969/j.issn.1006-267x.2014.11.028

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