Variation of Nutrients Flow and Recovery Rate of Inert Marker in Digesta Collected with T-Canuula at the Terminal Ileum of Growing Pigs

LIU Chengling, ZHAO Feng, ZUO Jianjun, WANG Yuming, ZHANG Li, GAO Lixiang, ZHANG Hongfu

doi:10.3969/j.issn.1006-267x.2014.11.029

2014 , Vol. 26 >Issue 11: 3404 - 3413

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.11.029

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Variation of Nutrients Flow and Recovery Rate of Inert Marker in Digesta Collected with T-Canuula at the Terminal Ileum of Growing Pigs

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1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Animal Science, South China Agriculture University, Guangzhou 510642, China

Received date: 2014-05-01

Online published: 2014-11-17

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Abstract

Representation of digesta samples is crucial to study on the in vivo digestion process of dietary nutrients in pigs, thereby, sampling scheme for representative digesta has to be based on the nutrients flow and the recovery of inert marker in digesta collected with T-cannula in pigs. In current experiment, 4 growing [(21.3±1.0) kg] pigs fitted with T-cannulas at the terminal ileum were fed with a corn-soybean meal diet in individual metabolic crate. Three digesta collection days for the 8th, 9th, 10th and 16th hourly digesta collection time from 08:00 to 24:00 per day were used in a 3×16 factorial arrangement. Hourly digesta was collected and weighed, then analyzed for gross energy and dry matter, crude protein and chromic oxide (Cr₂O₃) content. The results showed as follows: 1) using two-way ANOVA, collection time significantly affected the flow of fresh digesta, dry matter, gross energy, crude protein and Cr₂O₃ in cannula (P<0.05). No significant differences were observed for the daily mean flow in each of fresh digesta, dry matter, crude protein and Cr₂O₃ among 3 collection days (P>0.05), whereas, flow of gross energy was excluded (P<0.05). 2) The collection day significantly affected the accumulative flow of dry matter, gross energy, crude protein for 16 h in cannula and 24 h at terminal ileum (P<0.05). The both of accumulative flow in the 8th day of experiment period were greater than these in the 9th and 10th days (P<0.05), whereas no significant difference was observed for the 9th and 10th days (P>0.05). The accumulative flow of each nutrient for 16 h in T-cannula was greater than 68.0% of that passed the intestine in 24 h at terminal ileum. No significant difference was observed for this percentage among collection days (P>0.05).3) No significant daily difference was observed for the Cr₂O₃ flow collected for 16 h with T-cannula and the recovery rate of Cr₂O₃, respectively(P>0.05). The mean and coefficient of variation (CV) of recovery rate of Cr₂O₃ was 72.9% and 6.87%, respectively. In conclusion, during 3 days collection, a stable nutrients flow at terminal ileum is present in the last 2 days. Two continuous 8 hours collection after feeding can get satisfactory representative digesta sample. In a 3-day-collection period, the first day and following 2 days are suggested to be adaption and regular collection of digesta samples, respectively.

Key words： growing pigs; terminal ileum; digesta flow; recovery rate of index; variation

Cite this article

LIU Chengling, ZHAO Feng, ZUO Jianjun, WANG Yuming, ZHANG Li, GAO Lixiang, ZHANG Hongfu . Variation of Nutrients Flow and Recovery Rate of Inert Marker in Digesta Collected with T-Canuula at the Terminal Ileum of Growing Pigs[J]. Chinese Journal of Animal Nutrition, 2014 , 26(11) : 3404 -3413 . DOI: 10.3969/j.issn.1006-267x.2014.11.029

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