Effects of High Cottonseed Meal Diet Supplemented with Lysine and Iron on Growth, Immunity and Tissue Free Gossypol Content of Juvenile Black Carp (Mylopharyngodon piceus)

HU Yi, ZHANG Junzhi, HUANG Yun, XIAO Tiaoyi, ZHONG Lei, MI Haifeng, PENG Huizhen

doi:10.3969/j.issn.1006-267x.2014.11.034

2014 , Vol. 26 >Issue 11: 3443 - 3451

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.11.034

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Effects of High Cottonseed Meal Diet Supplemented with Lysine and Iron on Growth, Immunity and Tissue Free Gossypol Content of Juvenile Black Carp (Mylopharyngodon piceus)

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1. College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China;
2. Tongwei Co., Ltd., Chengdu 610041, China

Received date: 2014-05-07

Online published: 2014-11-17

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Abstract

An 8-week feeding trial was conducted to evaluate the effects of high cottonseed meal diet supplemented with lysine and iron on growth, immunity and tissue free gossypol content of juvenile black carp[initial average body weight of (7.01±0.05) g]. Five iso-nitrogenous and iso-energetic experimental diets were formulated, one diet contained 40% soybean meal (SBM) as the SBM control diet, one diet contained 41% cottonseed meal (CSM) as high CSM diet, and the other 3 diets supplemented with 0.342% crystalline lysine (effective content was 79%), 0.115% FeSO₄·7H₂O, 0.342% crystalline lysine+0.115% FeSO₄·7H₂O to replace equivalent wheat flour on the basis of high CSM diet, respectively. Each group had 3 replicates and each replicate had 30 fish. The results showed as follows: compared with SBM control group, the specific growth rate (SGR), red blood cell (RBC) count, serum complement 4 (C4) content and serum total superoxide dismutase (T-SOD) and lysozyme (LZM) activities in high CSM group were significantly reduced (P<0.05), serum glutamic-oxaloacetic aminotransferase (GOP) and glutamic pyruvic transaminase (GTP) activities in high CSM group were significantly increased (P<0.05), and the free gossypol was accumulated in liver. But, co-supplementation with lysine and iron could significantly increase the SGR and RBC count (P<0.05), and had no significant differences compared with SBM control group (P>0.05). Moreover, supplementation with iron could significantly increase serum C4 content, supplementation with lysine or iron could significantly increase serum LZM activity and significantly decrease serum GOP and GTP activities (P<0.05), and there were no significant differences in serum GOP and GTP activities between single supplementation with iron group and SBM control group (P>0.05). High CSM diet supplemened with iron could significantly decrease free gossypol content in muscle and liver (P<0.05), and supplemened with lysine could significantly increase serum T-SOD activity (P<0.05). To conclude, high CSM diet supplemented with lysine and iron can promote growth, enhance immunity and reduce free gossypol content in muscle and liver, while it is more efficient to be supplemented with lysine and iron simultaneously.

Key words： cottonseed meal; black carp (Mylopharyngodon piceus); lysine; iron; growth; immunity; free gossypol

Cite this article

HU Yi, ZHANG Junzhi, HUANG Yun, XIAO Tiaoyi, ZHONG Lei, MI Haifeng, PENG Huizhen . Effects of High Cottonseed Meal Diet Supplemented with Lysine and Iron on Growth, Immunity and Tissue Free Gossypol Content of Juvenile Black Carp (Mylopharyngodon piceus)[J]. Chinese Journal of Animal Nutrition, 2014 , 26(11) : 3443 -3451 . DOI: 10.3969/j.issn.1006-267x.2014.11.034

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