Effects of Dietary Glutamate on Muscle Quality of Grass Carp (Ctenopharyngodon idella) during Middle Growth Period

ZHAO Ye, ZHOU Xiaoqiu, HU Yi, LI Jiayong, LI Qian, FENG Lin, JIANG Weidan, LIU Yang, JIANG Jun

doi:10.3969/j.issn.1006-267x.2014.11.035

Chinese Journal of Animal Nutrition >

2014 , Vol. 26 >Issue 11: 3452 - 3460

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.11.035

Short Communications

Effects of Dietary Glutamate on Muscle Quality of Grass Carp (Ctenopharyngodon idella) during Middle Growth Period

Expand

1. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China;
2. Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China;
3. Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Ya'an 625014, China

Received date: 2014-05-18

Online published: 2014-11-17

Fold

Abstract

The present study was performed to investigate the effects of dietary glutamate on muscle quality of grass carp (Ctenopharyngodon idella) during middle growth period. A total of 540 grass carp with the initial body weight of (370.0±3.8) g were randomly allocated 3 groups with 3 replicates per group and 60 fish per replicate. The fish in the 3 groups were fed 3 experimental diets with the glutamate supplemental levels of 0 (control), 0.8% and 1.6%, respectively. The experiment lasted for 56 days. The results showed as follows: dietary supplementation with 0.8% and 1.6% glutamate significantly decreased the muscle fiber diameter (P<0.05), and significantly increased the muscle fiber density (P<0.05), but did not significantly affect the muscle cross sectional area and pH (P>0.05). Dietary supplementation with 0.8% and 1.6% glutamate significantly increased the muscle hardness, springiness, chewiness and gumminess (P<0.05), but did not significantly affect the muscle cohesiveness (P>0.05). Dietary supplementation with 0.8% and 1.6% glutamate did not significantly affect the composition of conventional nutrients and amino acids in muscle (P>0.05). The content of inosine monophosphate in muscle was significantly increased by dietary supplementation with 0.8% and 1.6% glutamate (P<0.05). Moreover, the relative expression level of adenylosuccinate lyase mRNA in muscle was significantly increased by dietary supplementation with 0.8% glutamate (P<0.05). These results indicate that dietary glutamate can improve the muscle fiber structure and muscle texture characteristics, and increase the content of muscle flavor substance—inosine monophosphate, which improve the muscle quality of grass carp during middle growth period.

Key words： grass carp (Ctenopharyngodon idella); muscle fiber density; texture characteristics; flavor substance

Cite this article

ZHAO Ye, ZHOU Xiaoqiu, HU Yi, LI Jiayong, LI Qian, FENG Lin, JIANG Weidan, LIU Yang, JIANG Jun . Effects of Dietary Glutamate on Muscle Quality of Grass Carp (Ctenopharyngodon idella) during Middle Growth Period[J]. Chinese Journal of Animal Nutrition, 2014 , 26(11) : 3452 -3460 . DOI: 10.3969/j.issn.1006-267x.2014.11.035

References

[1] MEGA J A.Umami flavor in meat[M]//SAHIDI F.Flavor of meat and meat products.Glasgow:Chapman & Hall,1994:98-115.

[2] LARSSON T,KOPPANG E O,ESPE M,et al.Fillet quality and health of Atlantic salmon (Salmo salar L.) fed a diet supplemented with glutamate[J].Aquaculture,2014,426/427:288-295.

[3] 蒋克勇.水产动物肉质风味改良技术及相关候选基因研究[D].博士学位论文.北京:中国科学院,2012.

[4] 马现永,周桂莲,林映才,等.饲粮中添加谷氨酸钠对黄羽肉鸡生长性能及肉品风味的影响[J].动物营养学报,2011,23(3):410-416.

[5] OEHME M,GRAMMES F,TAKLE H,et al.Dietary supplementation of glutamate and arginine to Atlantic salmon (Salmo salar L.) increases growth during the first autumn in sea[J].Aquaculture,2010,310(1/2):156-163.

[6] YAN L,XIAO Q Z.Dietary glutamine supplementation improves structure and function of intestine of juvenile Jian carp (Cyprinus carpio var.Jian)[J].Aquaculture,2006,256(1/2/3/4):389-394.

[7] PERIAGO M J,AYALA M D,LOPEZ-ALBORS O,et al.Muscle cellularity and flesh quality of wild and farmed sea bass,Dicentrarchus labrax L[J].Aquaculture,2005,249:175-188.

[8] ERKAN N,TOSUN S Y,ULUSOY S,et al.The use of thyme and laurel essential oil treatments to extend the shelf life of bluefish (Pomatomus saltatrix) during storage in ice[J].Journal für Verbraucherschutz und Lebensmittelsicherheit,2011,6(1):39-48.

[9] 关文静,朱艺峰,陈芝丹.鱼类肌纤维特性与鱼肉品质关系[J].水产科学,2008,27(2):101-104.

[10] ROWLERSON A,SCAPOLO P A,MASCARELLO F,et al.Comparative study of myosin present in the lateral muscle of some fish:species variations in myosin isoforms and their distribution in red,pink and white muscle[J].Journal of Muscle Research and Cell Motility,1985,6:601-640.

[11] VEGGETTI A,MASCARELLO F,SCAPOLO P A,et al.Hyperplastic and hypertrophic growth of lateral muscle in Dicentrarchus labrax (L.):an ultrastructural and morphometric study[J].Anatomy and Embryology,1990,182:1-10.

[12] KIESSLING A,STOREBAKKEN T,ASGARD T,et al.Changes in the structure and function of the epaxial muscle of rainbow trout (Oncorhynchus mykiss) in relation to ration and age:Ⅰ.Growth dynamics[J].Aquaculture,1991,93:335-356.

[13] JOHNSTON I A,MANTHRI S,ALDERSON R,et al.Effects of dietary protein level on muscle cellularity and flesh quality in Atlantic salmon with particular reference to gaping[J].Aquaculture,2002,210:259-283.

[14] LOVE R M.The food fishes:their intrinsic variation and practical implications[M].London:Farrand Press,1988.

[15] 高昕,韩芳,许加超,等.微冻贮藏条件下鲈鲜度和质构变化[J].水产学报,2010,34(8):1294-1302.

[16] HOULIHAN D F,CARTER C G,MCCARTHY I D.Chapter 8:protein synthesis in fish[J].Biochemistry and Molecular Biology of Fishes,1995,4:191-120.

[17] 朱健,王建新,龚永生,等.几种鲤鱼肌肉的一般营养成分及蛋白质氨基酸组成的比较[J].湛江海洋大学学报,2000,20(4):9-12.

[18] FUKE S,KONOSU S.Taste-active components in some foods:a review of Japanese research[J].Physiology & Behavior,1991,49(5):863-868.

[19] 徐善金,虞德兵,杜文兴.肌苷酸及其相关酶的研究进展[J].生物技术通报,2011(3):44-53.

[20] YUAN T,GU J R,GU W B,et al.Molecular cloning,characterization and expression analysis of adenylosuccinate lyase gene in grass carp (Ctenopharyngodon idella)[J].Molecular Biology Report,2011,38:2059-2065.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References