Effects of Water Soluble Material of Yeast Culture on Growth and Cell Membrane Integrity of Intestinal Epithelial Cells in Vitro of Grass Carp (Ctenopharyngodon idella)

YAO Shibin, YE Yuantu, CAI Chunfang, ZHANG Baotong, DONG Jiaojiao, HUANG Yuwei, CHEN Kequan

doi:10.3969/j.issn.1006-267x.2014.11.038

2014 , Vol. 26 >Issue 11: 3478 - 3484

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.11.038

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Effects of Water Soluble Material of Yeast Culture on Growth and Cell Membrane Integrity of Intestinal Epithelial Cells in Vitro of Grass Carp (Ctenopharyngodon idella)

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1. Key Laboratory of Aquatic Nutrition of Jiangsu Province, School of Biology and Basic Medical Sciences, Suzhou University, Suzhou 215123, China;
2. Open Laboratory for Aquatic Animal Nutrition, Beijing Research Institute for Nutritional Resources, Beijing 100069, China

Received date: 2014-05-14

Online published: 2014-11-17

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Abstract

In this experiment, water soluble material of yeast culture as the experimental material was added in the culture solution of intestinal epithelial cells in vitro of grass carp (Ctenopharyngodon idella), in order to study the effects of water soluble material of yeast culture on growth and cell membrane integrity under the different concentrations and different action time of water soluble material of yeast culture. One control group and 5 water soluble material of yeast culture groups (YC-1 to 5 groups) were designed by using single-factor experimental design, and each group had 96 replicates and 1 replicate was a culture hole. The control group was not added the water soluble material of yeast culture, and the concentration of water soluble material of yeast culture in YC-1 to 5 groups was 10, 25, 50, 100 and 200 mg/L, respectively. The results showed as follows: the cell morphology was no injury when adding 50 to 200 mg/L water soluble material of yeast culture into the culture solution, the cell activity was increased on 3 h after added 100 to 200 mg/L water soluble material of yeast culture, and the cell activity was increased on 6 h after added 50 mg/L water soluble material of yeast culture. Compared with control group, the activity of lactate dehydrogenase (LDH) in culture solution in all water soluble material of yeast culture groups had no significant change on 3 h (P>0.05), while that in YC-4 and YC-5 groups was significantly reduced on 6 h (P<0.05), that in YC-3, YC-4 and YC-5 groups was significantly reduced on 9 h (P<0.05), and that in YC-2, YC-3, YC-4 and YC-5 groups was significantly reduced on 12 h (P<0.05). The activities of glutamic-pyruvic transaminase (GPT) and glutamic-oxaloacetic transaminase (GOT) in culture solution in all water soluble material of yeast culture groups had no significant change on all time points (3, 6, 9 and 12 h) compared with control group (P>0.05), but the activity of GOP on 6 to 9 h in all water soluble material of yeast culture groups was lower than that in control group. In conclusion, adding 10 to 200 mg/L water soluble material of yeast culture into the culture solution can improve the growth and protect the cell membrane integrity of intestinal epithelial cells in vitro of grass carp, and the appropriate concentration of playing protective effect is 50 to 200 mg/L.

Key words： grass carp (Ctenopharyngodon idella); intestinal epithelial cells; water soluble material of yeast culture; cell growth; cell membrane integrity

Cite this article

YAO Shibin, YE Yuantu, CAI Chunfang, ZHANG Baotong, DONG Jiaojiao, HUANG Yuwei, CHEN Kequan . Effects of Water Soluble Material of Yeast Culture on Growth and Cell Membrane Integrity of Intestinal Epithelial Cells in Vitro of Grass Carp (Ctenopharyngodon idella)[J]. Chinese Journal of Animal Nutrition, 2014 , 26(11) : 3478 -3484 . DOI: 10.3969/j.issn.1006-267x.2014.11.038

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