Expression of Glucose Transporter 4 and Glucose Transporter 2 in Different Tissues of Tilapia and Its Response to Glucose Injection

LIU Hanliang, WANG Jiting, WAN Wenju, FU Peisheng, SUN Minmin, WANG Hongwei

doi:10.3969/j.issn.1006-267x.2014.11.041

2014 , Vol. 26 >Issue 11: 3500 - 3509

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.11.041

Short Communications

Expression of Glucose Transporter 4 and Glucose Transporter 2 in Different Tissues of Tilapia and Its Response to Glucose Injection

Expand

1. College of Animal Science and Technology, Shandong Agricultural University, Taian 271000, China;
2. School of Basic Medical Sciences, Taishan Medical University, Taian 271000, China;
3. Freshwater Fishery Research Institute of Shandong Province, Jinan 252000, China

Received date: 2014-05-23

Online published: 2014-11-17

Fold

Abstract

This experiment was conducted to determine the expression of glucose transporter (GLUT) 4 and GLUT2 in different tissues of tilapia (Oreochromis niloticus) and its response to glucose injection. A partial cDNA of GLUT4 was cloned from muscle of tilapia by real-time PCR method. The partial cDNA of GLUT4 (GenBank accession No. was JN900493) was 603 bp, and encoded 200 amino acids. The expression differences of GLUT4 and GLUT2 in muscle, heart and liver were compared by semi-quantitative real-time PCR detection. The results showed that the expression level of GLUT4 in muscle and heart was higher, while that in liver was lower; the expression level of GLUT2 in liver was higher, while that in muscle and heart was very low. A total of 150 tilapia with the body weight about 80 g were randomly allotted to 2 groups with 3 replicates per group and 25 fish per replicate. In experimental group, glucose with the dose of 30 mg per 100 g body weight was injected intraperitoneally into the fish, while the fish in control group were injected with 0.7% sterile saline water as the same dose and method. Samples were collected and measured before infection (0 h) and at 1, 3, 6 and 12 h after infection, respectively. The results showed as follows:1) the maximum plasma glucose content of experimental group was observed at 1 h after injection which was significantly higher than that of control group (P<0.05), and then began to decreaseed and returned to normal level at 3 h after injection; the maximum plasma insulin content of experimental group was observed at 3 h after injection which was significantly higher than that of control group (P<0.05), and then began to decrease and returned to normal level at 12 h after injection. 2)The relative expression level of GLUT4 mRNA in muscle of experimental group began to increase at 3 h after injection, and reached the maximum at 12 h after injection which was significantly higher than that of control group (P<0.05); the relative expression level of GLUT4 mRNA in heart at 1 h after injection of experimental group was significantly higher than that of control group (P<0.05), and then decreased gradually with the passage of time and return to normal level at 6 h after injection; the relative expression level of GLUT4 mRNA in liver had no significant change after glucose injection (P>0.05). The results suggest that injecting glucose can instantaneously increase the plasma glucose content of tilapia, but the increase of plasma insulin content is relatively delayed compare with plasma glucose content, and the relative expression level of GLUT4 mRNA in muscle show a delayed increase relative to plasma insulin content, then the burden of glucose metabolism in tilapia is increased.

Key words： tilapia (Oreochromis niloticus); glucose; glucose transporter 4; glucose transporter 2

Cite this article

LIU Hanliang, WANG Jiting, WAN Wenju, FU Peisheng, SUN Minmin, WANG Hongwei . Expression of Glucose Transporter 4 and Glucose Transporter 2 in Different Tissues of Tilapia and Its Response to Glucose Injection[J]. Chinese Journal of Animal Nutrition, 2014 , 26(11) : 3500 -3509 . DOI: 10.3969/j.issn.1006-267x.2014.11.041

References

[1] GOULD G W,HOLMAN G D.The glucose transporter family:structure,function and tissue-specific expression[J].Biochemical Journal,1993,295(Pt 2):329-341.

[2] JOOST H G,THORENS B.The extended GLUT-family of sugar/polyol transport facilitators:nomenclature,sequence characteristics,and potential function of its novel members(review)[J].Molecular Membrane Biology,2001,18(4):247-256.

[3] JAMES R,WRIGHT J R,BONEN A,et al.Glucose homeostasis in the teleost fish tilapia:insights from Brockmann body xenotransplantation studies[J].American Zoologist,2000,40(2):234-245.

[4] SHIMENO S,KHEYY D,SHIKATA T.Metabolic response to dietary carbohydrate to protein ratios in carp[J].Fisheries Science,1995,61:277-281.

[5] 蔡春芳,王道尊.异育银鲫对糖利用性的研究——外源胰岛素敏感性试验[J].中国水产科学,1999,6(1):62-65.

[6] PANSERAT S,PLAGNES J E,BREQUE J.Hepatic phosphoenolpyruvate carboxykinase gene expression is not repressed by dietary carbohydrates in rainbow trout (Oncorhynchus mykiss)[J].The Journal of Experimental Biology,2001,204(2):359-365.

[7] 林小植,罗毅平,谢小军.饲料碳水化合物水平对南方鲇幼鱼餐后糖酵解酶活性及血糖浓度的影响[J].水生生物学报,2006,30(3):304-311.

[8] 罗毅平.肉食性鱼类南方鲇对饲料碳水化合物营养胁迫的生理生态学反应[D].博士学位论文.重庆:西南大学,2007.

[9] CARNEIRO N M,NAVARRO I,GUTIRREZ J,et al.Hepatic extraction of circulating insulin and glucagon in brown trout (Salmo trutta fario) after glucose and arginine injection[J].Journal of Experimental Zoology,1993,267(4):416-422.

[10] HRUZ P W,MUECKLER M M.Structural analysis of the GLUT1 facilitative glucose transporter[J].Molecular Membrane Biology,2001,18(3):183-193.

[11] CAPILLA E,DIAZ M,GUTIERREZ J,et al.Physiological regulation of the expression of a GLUT4 homolog in fish skeletal muscle[J].American Journal of Physiology Endocrinology and Metabolism,2002,283:E44-E49.

[12] WOOD I S,TRAYHURN P.Glucose transporters (GLUT and SGLT):expanded families of sugar transport proteins[J].British Journal of Nutrition,2003,89(1):3-9.

[13] MOON T W.Glucose intolerance in teleost fish:fact or fiction?[J].Comparative Physiology and Biochemistry,2001,129(2/3):243-249.

[14] HALL J R,SHORT C E,DRIEDZIC W R.Sequence of Atlantic cod (Gadus morhua) GLUT4,GLUT2 and GPDH:developmental stage expression,tissue expression and relationship to starvation-induced changes in blood glucose[J].The Journal of Experimental Biology,2006,209(22):4490-4502.

[15] TEERIJOKI H,KRASNOV A,PITKANEN T I,et al.Cloning and characterization of glucose transporter in teleost fish rainbow trout (Oncorhynchus mykiss)[J].Biochimica et Biophysica Acta,2000,1494(3):290-294.

[16] PLANAS J V,CAPILLA E,GUTIRREZ J.Molecular identification of a glucose transporter from fish muscle[J].FEBS Letters,2000,481(3):266-270.

[17] PAUL F P,JONGSOON L.Insulin receptor family[J].Encyclopedia of Biological Chemistry,2004,2:436-440.

[18] DÍAZ M,CAPILLA E,PLANAS J V.Physiological regulation of glucose transporter (GLUT4) protein content in brown trout (Salmo trutta) skeletal muscle[J].The Journal of Experimental Biology,2007,210(13):2346-2351.

[19] BRYANT N J,GOVERS R,JAMES D E.Regulated transport of the glucose transporter GLUT4[J].Nature Reviews Molecular Cell Biology,2002,3(4):267-277.

[20] PESSIN J E,THURMOND D C,ELMENDORF J S,et al.Molecular basis of insulin-stimulated GLUT4 vesicle trafficking[J].Journal of Biological Chemistry,1999,274(5):2593-2596.

[21] MOMMSEN T P,PLISETSKAYA E M.Insulin in fishes and agnathans:history,structure and metabolic regulation[J].Reviews in Aquatic Sciences,1991,4:225-259.

[22] CAPILLA E,DÍAZ M,ALBALAT A,et al.Functional characterization of an insulin-responsive glucose transporter (GLUT4) from fish adipose tissue[J].American Journal of Physiology:Endocrinology and Metabolism,2004,287(2):E348-E357.

[23] WRIGHT E M,TURK E M.The sodium glucose cotransporter family SLC5[J].Pflugers Arch,2003,447:813-815.

[24] KRASNOV A,TEERIJOKI H,MOLSA H.Rainbow trout (Onchorhynchus mykiss) hepatic glucose transporter[J].Biochimica et Biophysica Acta:Gene Structure and Expression,2001,1520(2):174-178.

[25] ZHAO F Q,KEATING A F.Functional properties and genomics of glucose transporters[J].Current Genomics,2007,8(2):113-128.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References