Effects of Glucose and Vitamin C Inhabitation on Activities of Acetyl-CoA Carboxylase,Fatty Acid Synthase and Carnitine Palmitoyltransferases Ⅰ during Embryo Development of Carassius auratus gibelio

JIANG Zuoyu, YAO Junjie, AN Miao, XIONG Hualong, ZHU Zhongsheng

doi:10.3969/j.issn.1006-267x.2014.11.042

2014 , Vol. 26 >Issue 11: 3510 - 3516

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.11.042

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Effects of Glucose and Vitamin C Inhabitation on Activities of Acetyl-CoA Carboxylase,Fatty Acid Synthase and Carnitine Palmitoyltransferases Ⅰ during Embryo Development of Carassius auratus gibelio

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Department of Fisheries Science of College of Animal Sciences, Guizhou University, Guiyang 550025, China

Received date: 2014-05-01

Online published: 2014-11-17

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Abstract

In order to study the changes of activities of acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), and carnitine palmitoyltransferase Ⅰ (CPTⅠ), and the effects of glucose and vitamin C inhabitation on them during embryo development of Carassius auratus gibelio (C. auratus), glucose solution and vitamin C solutions with different concentrations were used for hatching. The concentrations of glucose were 0, 5, 10, 15 and 20 g/L, respectively, and the concentrations of vitamin C were 0, 20, 25, 30 and 35 mg/L, respectively. Membrane break time and hatching rate were recorded to decide the optimal concentrations of glucose and vitamin C. Solutions without addition (control group) and with optimal concentrations of glucose (glucose group) or vitamin C (vitamin C group) were used for hatching, and the characteristics of changes of ACC, FAS and CPTⅠ activities were analyzed during embryo development. The results showed as follows: 1) membrane break time was the shortest, and hatching rate was the highest when the concentrations of glucose and vitamin C were 15 g/d and 30 mg/L, respectively. 2) The specific activity and total activity of ACC, FAS and CPTⅠ showed increasing tends during embryo development of C. auratus. 3) The specific activities and total activities of ACC and FAS in glucose group were significantly higher than those in control group at mid-gastrul, crystal appear and prehatching stages (P<0.05), and the specific activity an total activity of CPTⅠ was significantly higher than that in control group at crystal appear and prehatching stages (P<0.05). 4) The total activities of ASS and FAS in vitamin C group were significantly higher than those in control group (P<0.05). In conclusion, the inhabitation in solutions with appropriate concentrations of glucose (15 g/L) and vitamin C (30 mg/L) can promote synthesis and secretion of ACC, FAS and CPTⅠ during embryo development of C. auratus, and form new metabolic levels to maintain the dynamic balance of lipid metabolism in embryo.

Key words： glucose; vitamin C; Carassius auratus; embryo; acetyl-CoA carboxylase; fatty acid synthase; carnitine palmitoyltransferase Ⅰ

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JIANG Zuoyu, YAO Junjie, AN Miao, XIONG Hualong, ZHU Zhongsheng . Effects of Glucose and Vitamin C Inhabitation on Activities of Acetyl-CoA Carboxylase,Fatty Acid Synthase and Carnitine Palmitoyltransferases Ⅰ during Embryo Development of Carassius auratus gibelio[J]. Chinese Journal of Animal Nutrition, 2014 , 26(11) : 3510 -3516 . DOI: 10.3969/j.issn.1006-267x.2014.11.042

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