This experment was conducted to study the effects of dietary copper level on copper apparent biological utilization and copper deposition in tissue and organ of silver foxes during the winter hair period. Fifty 120-day-old female silver foxes with an average body weight of (5 134±540) g were selected and randomly assigned into 5 groups with 10 replicates in each group and 1 silver fox in each replicate. Foxes in the 6 groups were fed the basal diets supplemented 6 (group Ⅰ), 30 (group Ⅱ), 60 (group Ⅲ), 90 (group Ⅳ) and 150 mg/kg (group Ⅴ) citric acid copper, respectively. The copper content in basal diet was 4.92 mg/kg. The pre-trial period lasted for 7 days and the trial period lasted for 51 days. The results showed as follows: 1) dietary copper level did not significantly affect average daily feed intake, fecal output and copper apparent biological utilization (P>0.05), but significantly affected the copper intake and fecal copper output (P<0.01). The copper intake and fecal copper output were significantly increased with dietary copper level increasing (P<0.01). 2) Dietary copper level significantly affected the copper deposition in hair, liver, kidney and heart (P<0.01). The hair copper deposition were increased with dietary copper level increasing, and that of group Ⅴ was the highest. There was no regular change on copper deposition in liver, kidney and heart. 3) Dietary copper level significantly affected the heart weight (P<0.05), from group Ⅱ to group Ⅴ, heart weight had a declined trend. Dietary copper level did not significantly affect liver weight, kidney weight and spleen weight (P>0.05). It is concluded that adding 60 mg/kg citric acid copper (actual dietary copper content appropriately 65 mg/kg) in the basal diet is beneficial for digestion and utilization of copper of silver foxesx during the winter hair period.