Ruminant Nutrition

Compositon and Diversity of Ruminal Methanogens in Guangxi Fuzhong Buffaloes: an Analysis of Using 16S rRNA Gene Cloning Library Technique

  • YANG Chengjian ,
  • LIANG Xin ,
  • WEI Shengju ,
  • LI Shulu ,
  • LIANG Xianwei ,
  • ZOU Caixia ,
  • YANG Bingzhuang ,
  • LI Lili
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  • Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture (Guangxi), Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China

Received date: 2014-06-01

  Online published: 2014-12-05

Abstract

The objective of this study was to analysis the composition and the diversity of ruminal methanogens in Guangxi Fuzhong buffaloes by using 16S rRNA gene cloning library technique. Three healthy female Fuzhong buffaloes with similar body condition were used in this study. Total DNA of ruminal contents was extracted by bead-beating method. Primers of Met86F/Met1340R of methanogens were used to amplify 16S rRNA gene for the construction of 16S rRNA gene clone library. The results showed that 93 chimera-free 16S rRNA sequences were obtained in the present study. Based the 97% similarity, these sequences were assigned to 39 operational taxonomic units (OTUs). Among those, sixty sequences showed ≥97% sequence similarity to known species (15 OTUs, occupied 64.5% of total sequences), thirty two sequences had sequence similarity to known species in the range of 90% to <97% (23 OTUs), and only one sequence displayed similarity <90% with Methanomassiliicoccus luminyensis. Phylogenetic analysis indicated that 98.9% sequences of methanogens in the rumen of Fuzhong buffaloes belonged to Methanobacteriales. Some sequences were far from all known species on the phylogenetic tree. They might represent new genus or species in Methanobacteriales in the rumen of Fuzhong buffaloes. It can be concluded that Methanobacteriales is the dominant methanogens in the rumen of Fuzhong buffaloes. Further study should be done for the isolation of unknown methanogens and their function analysis.

Cite this article

YANG Chengjian , LIANG Xin , WEI Shengju , LI Shulu , LIANG Xianwei , ZOU Caixia , YANG Bingzhuang , LI Lili . Compositon and Diversity of Ruminal Methanogens in Guangxi Fuzhong Buffaloes: an Analysis of Using 16S rRNA Gene Cloning Library Technique[J]. Chinese Journal of Animal Nutrition, 2014 , 26(12) : 3635 -3642 . DOI: 10.3969/j.issn.1006-267x.2014.12.012

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