This experiment was conducted to compare the effects of sodium selenite and selenium yeast on performance, egg quality and egg selenium content of laying hens. Three hundred and sixty 30-week-old healthy Hy-Line Brown laying hens with a similar laying rate were randomly assigned to 5 groups with 6 replicates in each group and 12 laying hens per replicate. The control groups was fed a basal diet without adding selenium, while the diets of experimental groups were fed the basal diet supplemented with sodium selenite containing 0.30 mg/kg selenium and selenium yeast containing 0.10, 0.20 and 0.30 mg/kg selenium, respectively. The measured values of selenium content in experimental diets were 0.176, 0.342, 0.278, 0.302 and 0.359 mg/kg, respectively. Pre-trail period was 14 days and experimental period was 28 days. The results showed as follows: 1) there were no significant differences in the laying rate, average egg weight, average daily egg weight, average daily feed intake, ratio of feed to egg, and egg quality among the 5 groups (P>0.05). 2) Compared with the control group, the addition of sodium selenite and selenium yeast in the basal diet could significantly increase egg selenium content (P<0.05). The egg selenium content in the groups supplemented with selenium yeast was significantly higher than that in the group supplemented with sodium selenite (P<0.05). The egg selenium content increased significantly with the addition of selenium yeast increased (P<0.05). In conclusion, the selenium absorption and conversion rate of selenium yeast are significantly higher than those of sodium selenite. The addition of selenium in the diet can significantly increase egg selenium content and the egg selenium content increase significantly with the addition of selenium yeast increased. No harmful effect is found in performance and egg quality of laying hens feed with the diet supplemented with 0.10 to 0.30 mg/kg selenium yeast.