Effects of Different Types and Levels of Oil on the Expression of 18S rRNA in Broilers

ZHANG Wenwen; LU Weiwei; WANG Ting; ZHAO Yiwen; ZHANG Pengfei; WANG Xianyong; CHEN Wen; HUANG Yanqun

doi:10.3969/j.issn.1006-267x.2014.12.035

Chinese Journal of Animal Nutrition >

2014 , Vol. 26 >Issue 12: 3814 - 3820

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.12.035

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Effects of Different Types and Levels of Oil on the Expression of 18S rRNA in Broilers

ZHANG Wenwen ,
LU Weiwei ,
WANG Ting ,
ZHAO Yiwen ,
ZHANG Pengfei ,
WANG Xianyong ,
CHEN Wen ,
HUANG Yanqun

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1. Animal Husbandry Medical College of Engineering, Henan Agricultural University, Zhengzhou 450002, China;
2. Shang Du Biological Technology Co., Ltd. of Henan, Zhengzhou 466311, China

Received date: 2014-06-30

Online published: 2014-12-05

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Abstract

This experiment was conducted to study the effects of different types and levels of oil on the expression of 18S rRNA in liver and chest muscle of broilers. A total of 240 1-day-old female Cobb-500 broilers were fed in this experiment. Broilers were randomly assigned to 8 groups (fed basal diets added with 2.50% and 5.00% linseed oil, corn oil, sesame oil and lard oil, respectively) with 6 replicates per group and 5 broilers per replicate. One broiler of each replicate was selected to slaughter at age of 42 days. The results showed as follows: 1) the relative expression levels of 18S rRNA in liver and breast muscle of broilers were both significantly affected by the oil type and level (P<0.01), and the interactive effect of oil type and level also had significantly effect on the relative expression level of 18S rRNA in liver of broilers (P<0.01). 2) Compared with oil level of 2.50%, except 5.00% linseed level did not significantly affect relative expression level of 18S rRNA in liver of broilers (P>0.05), the increasing of oil level (5.00%) significantly increased the relative expression level of 18S rRNA in liver and breast muscle of broilers (P<0.05 or P<0.01). 3) The relative expression levels of 18S rRNA of linseed oil groups were higher than corn oil, sesame oil and lard oil groups both in liver and breast muscle of broilers at varying degrees (P<0.05 or P>0.05). The results of this study show that the 18S rRNA expression in liver and breast muscle of broilers is affected by both oil type and level.

Key words： broilers; oil; 18S rRNA; gene expression

Cite this article

ZHANG Wenwen , LU Weiwei , WANG Ting , ZHAO Yiwen , ZHANG Pengfei , WANG Xianyong , CHEN Wen , HUANG Yanqun . Effects of Different Types and Levels of Oil on the Expression of 18S rRNA in Broilers[J]. Chinese Journal of Animal Nutrition, 2014 , 26(12) : 3814 -3820 . DOI: 10.3969/j.issn.1006-267x.2014.12.035

References

[1] 许才康,孙华,马红梅.食用油脂的组份及其产品的优化[J].浙江农业科学,2001(5):259-261.

[2] 尤新.玉米油的营养功能和发展前景[J].粮油食品科技,2004,12(2):21-22.

[3] 黄玉华,邓泽元.芝麻中脂肪酸成分的GC分析[J].现代食品科技,2006,22(2):225-227.

[4] 安文俊,张旭晖,董丽,等.不同配比油脂对肉鸡生产性能的影响[J].饲料研究,2011(3):1-5.

[5] FRITSCHE K L,CASSITY N A,HUANG S C.Effect of dietary fat source on antibody production and lymphocyte proliferation in chickens[J].Poultry Science,1991,70(3):611-617.

[6] TSUJI N,KAMAGATA C,FURUYA M,et al.Selection of an internal control gene for quantitation of mRNA in colonic tissues[J].Anticancer Research,2002,22(6C):4173-4178.

[7] FUSTER G,VICENTE R,COMA M,et al.One-step reverse transcription polymerase chain reaction for semiquantitative analysis of mRNA expression[J].Methods and Findings in Experimental and Clinical Pharmacology,2002,24(5):253-259.

[8] BAS A,FORSBERG G,HAMMARSTROM S,et al.Utility of the housekeeping genes 18S rRNA,β-actin and glyceraldehyde-3-phosphate-dehydrogenase for normalization in real-time quantitative reverse transcriptase-polymerase chain reaction analysis of gene expression in Human T lymphocytes[J].Scandinavian Journal of Immunology,2004,59(6):566-573.

[9] THELLIN O,ZORZI W,LAKAYE B,et al.Housekeeping genes as internal standards:use and limits[J].Journal of Biotechnology,1999,75(2/3):291-295.

[10] TANIC N,PEROVIC M,MLADENOVIC A,et al.Effects of aging,dietary restriction and glucocorticoid treatment on housekeeping gene expression in rat cortex and hippocampus-Evaluation by real time RT-PCR[J].Journal of Molecular Neuroscience,2007,32(1):38-46.

[11] HAYASHI R,HAYASHI S,ARAI K,et al.Effects of antioxidant supplementation on mRNA expression of glucose-6-phosphate dehydrogenase,β-actin and 18S rRNA in the anterior capsule of the lens in cataract patients[J].Experimental Eye Research,2012,96(1):48-54.

[12] 叶文静,侯配强,赵晓民,等.荧光定量聚合酶链反应法比较标准管家基因3-磷酸甘油醛脱氢酶、β-肌动蛋白、酸性核糖体磷蛋白P0及18S核糖体RNA在老年大鼠不同组织中的表达[J].中国组织工程研究与临床康复,2008,12(18):3515-3518.

[13] 杨小军.日粮油脂调控肉鸡肠道粘膜免疫和免疫应激信号传导的研究[D].博士学位论文.北京:中国农业大学,2006:52-63.

[14] 陈文.油脂脂肪酸组成对肉鸡生产/屠宰性能及脂质代谢调控机理的研究[D].博士学位论文.北京:中国农业大学,2011:16-19.

[15] LI S Y,CHEN W,KANG X T,et al.Distinct tissue expression profiles of chicken Lpin1-α/β isoforms and the effect of the variation on muscle fiber traits[J].Gene,2013,515(2):281-290.

[16] BUSTIN S A.Quantification of mRNA using real-time reverse transcription PCR (RT-PCR):trends and problems[J].Journal of Molecular Endocrinology,2002,29(1):23-39.

[17] JUMP D B,TRIPATHY S,DEPNER C M.Fatty acid-regulated transcription factors in the liver[J].Annual Review of Nutrition,2013,33(1):249-269.

[18] 陆春瑞.共轭亚油酸对肉鸡生产性能及腹脂相关基因的影响[D].硕士学位论文.合肥:安徽农业大学,2007:23-26.

[19] TAKAHASHI Y,KUSHIRO M,SHINOHARA K,et al.Activity and mRNA levels of enzymes involved in hepatic fatty acid synthesis and oxidation in mice fed conjugated linoleic acid[J].Biochimica et Biophysisa Acta (BBA)-Molecular and Cell Biology of Lipids,2003,1631(3):265-273.

[20] MANDIMIKA T,PATURI G,GUZMAN E C,et al.Effects of dietary broccoli fibre and corn oil on serum lipids,faecal bile acid excretion and hepatic gene expression in rats[J].Food Chemistry,2012,131(4):1272-1278.

[21] KUCHIPUD S V,TELLABATI M,NELLI R K,et al.18S rRNA is a reliable normalisation gene for real time PCR based on influenza virus infected cells[J].Virology Journal,2012,9(1):230-236.

[22] XU J,NAKAMURA M T,CHO H P,et al.Sterol regulatory element binding protein-1 expression is suppressed by dietary polyunsaturated fatty acids[J].The Journal of Biological Chemistry,1999,274(33):23577-23583.

[23] 张艳君,朱志峰,陆融,等.基因表达转录分析中内参基因的选择[J].生物化学与生物物理进展,2007,34(5):546-550.

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