Effects of &beta;-Hydroxybutyric Acid on Expressions of Genes Involved in Milk Protein Synthesis in Bovine Mammary Epithelial Cells

WANG Xinpeng; YAN Sumei; QI Lizhi; SHENG Ran; ZHAO Yanli

doi:10.3969/j.issn.1006-267x.2014.12.038

2014 , Vol. 26 >Issue 12: 3836 - 3842

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.12.038

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Effects of β-Hydroxybutyric Acid on Expressions of Genes Involved in Milk Protein Synthesis in Bovine Mammary Epithelial Cells

WANG Xinpeng ,
YAN Sumei ,
QI Lizhi ,
SHENG Ran ,
ZHAO Yanli

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College of Animal Science, Inner Mongolia Agricultural University, Huhhot 010018, China

Received date: 2014-06-27

Online published: 2014-12-05

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Abstract

The purpose of this study was to examine the effects of β-hydroxybutyric acid (BHBA) on expressions of genes involved in milk protein synthesis in bovine mammary epithelial cells (BMECs). The 3rd passage BMECs with proper densities were cultured for 48 h, then were divided into 6 groups with 6 replicates in each group and 1 culture pore per replicate. Culture mediums containing different concentrations of BHBA [0 (control group), 0.58, 1.16, 2.32, 4.64 and 9.28 mmol/L, respectively] were used in each group. Cells were continuous incubated in an incubator with 5% CO₂ at 37 ℃ for 48 h, then were collected for the analysis of expressions of milk protein and the expression of related genes in real-time fluorescent quantitative PCR with SYBR Green. The results showed as follows: with the increasing of BHBA concentration, the expression of CSN1S1 gene was significantly increased quadratically (R²=0.931 6, P=0.018), 2.32 to 9.28 mmol/L had better effect, and 4.64 mmol/L was optimal concentration. With the increasing of BHBA concentration, leptin gene expression was linearly down-regulated at a certain extent (R²=0.482 5, P=0.126), and 0.58 mmol/L was optimal concentration in value. With the increasing of BHBA concentration, signal transducer and activator of transcription 5 (STAT5) and mammalian target of rapamycin (mTOR) gene expression were not significantly changed (P=0.459, P=0.398). Taken together, the expression of CSN1S1 gene is up-regulated when cells areincubated with BHBA at 2.32 to 9.28 mmol/L, and the synthesis of milk proteins in BMECs has better effect when the concentration of BHBA is 4.64 mmol/L.

Key words： β-hydroxybutyric acid; dairy cow; bovine mammary epithelial cells; milk protein

Cite this article

WANG Xinpeng , YAN Sumei , QI Lizhi , SHENG Ran , ZHAO Yanli . Effects of β-Hydroxybutyric Acid on Expressions of Genes Involved in Milk Protein Synthesis in Bovine Mammary Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2014 , 26(12) : 3836 -3842 . DOI: 10.3969/j.issn.1006-267x.2014.12.038

References

[1] JENKINS T C,MCGUIRE M A.Major advances in nutrition:impact on milk composition[J].Journal of Dairy Science,2006,89(4):1302-1310.

[2] CANT J P,DEPETERS E J,BALDWIN R L.Effect of dietary fat and postruminal casein administration on milk composition of lactating dairy cows[J].Journal of Dairy Science,1991,74(1):211-219.

[3] YONEZAWA T,YONEKURA S,KOBAYASHI Y,et al.Effects of long-chain fatty acids on cytosolic triacylglycerol accumulation and lipid droplet formation in primary cultured bovine mammary epithelial cells[J].Journal of Dairy Science,2004,87(8):2527-2534.

[4] WEISBJERG M R,WIKING L,KRISTENSEN N B,et al.Effects of supplemental dietary fatty acids on milk yield and fatty acid composition in high and medium yielding cows[J].The Journal of Dairy Research,2008,75(2):142-152.

[5] MAXIN G,GLASSER F,HURTAUD C,et al.Combined effects of trans-10,cis-12 conjugated linoleic acid,propionate,and acetate on milk fat yield and composition in dairy cows[J].Journal of Dairy Science,2011,94(4):2051-2059.

[6] 齐利枝,生冉,闫素梅,等.乙酸浓度对奶牛乳腺上皮细胞甘油三酯含量及瘦素和过氧化物酶增殖物激活受体γ基因表达量的影响[J].动物营养学报,2013,25(7):1519-1525.

[7] QI L Z,YAN S M,SHENG R,et al.Effects of saturated long-chain fatty acid on Mrna expression of genes associated with milk fat and protein biosynthesis in bovine mammary epithelial cells[J].Asian-Australasian Journal of Animal Sciences, 2014,27(3):414-421.

[8] 孔庆洋,林叶,李庆章.乙酸钠和丁酸钠对奶牛乳腺脂肪酸合成相关基因的影响[J].中国乳品工业,2012,40(3):15-17.

[9] LAPLANTE M,SABATINI D M.mTOR signaling at a glance[J].Journal of Cell Science,2009,122(20):3589-3594.

[10] BURGOS S A,DAI M,CANT J P.Nutrient availability and lactogenic hormones regulate mammary protein synthesis through the mammalian target of rapamycin signaling pathway[J].Journal of Dairy Science,2010,93(1):153-161.

[11] HENNIGHAUSEN L.Molecular mechanisms of hormone controlled gene expression in the breast[J].Molecular Biology Reports,1997,24(3):169-174.

[12] 胡菡.中国荷斯坦奶牛乳腺上皮细胞体外培养体系的建立与应用[D].博士学位论文.兰州:甘肃农业大学,2010.

[13] HU H,WANG J Q,BU D P,et al.In vitro culture and characterization of a mammary epithelial cell line from Chinese Holstein dairy cow[J].PLoS ONE,2009,4(11):e7636.

[14] ZHOU Y,AKERS R M,JIANG H.Growth hormone can induce expression of four major milk protein genes in transfected MAC-T cells[J].Journal of Dairy Science,2008,91(1):100-108.

[15] FEUERMANN Y,MABJEESH S J,SHAMAY A.Leptin affects prolactin action on milk protein and fat synthesis in the bovine mammary gland[J].Journal of Dairy Science,2004,87(9):2941-2946.

[16] 顾瑞霞.乳与乳制品的生理功能特性[M].北京:中国轻工业出版社,2001:1-5.

[17] SOLIMAN M M,AHMED M M,SALAH-ELDIN A,et al.Butyrate regulates leptin expression through different signaling pathways in adipocytes[J].Journal of Veterinary Science,2011,12(4):319-323.

[18] CHILLIARD Y,DELAVAUD C,BONNET M.Leptin expression in ruminants:nutritional and physiological regulations in relation with energy metabolism[J].Domestic Animal Endocrinology,2005,29(1):3-22.

[19] YONEZAWA T,YONEKURA S,SANOSAKA M,et al.Octanoate stimulates cytosolic triacylglycerol accumulation and CD36 mRNA expression but inhibits acetyl coenzyme A carboxylase activity in primary cultured bovine mammary epithelial cells[J].The Journal of Dairy Research,2004,71(4):398-404.

[20] SOLIMAN M,KIMURA K,AHMED M,et al.Inverse regulation of leptin mRNA expression by short- and long-chain fatty acids in cultured bovine adipocytes[J].Domestic Animal Endocrinology,2007,33(4):400-409.

[21] XIONG Y M,MIYAMOTO N,SHIBATA K,et al.Short-chain fatty acids stimulate leptin production in adipocytes through the G protein-coupled receptor GPR41[J].Proceedings of National Academy of Sciences of United States of America,2004,101(4):1045-1050.

[22] BROWN A J,GOLDSWORTHY S M,BARNES A A,et al.The orphan g protein-coupled receptors GPR41 and GPR43 are activated by propionate and other short chain carboxylic acids[J].The Journal of Biological Chemistry,2003,278(13):11312-11319.

[23] KADEGOWDA A K G,PIPEROVA L S,DELMONTE P,et al.Abomasal infusion of butterfat increases milk fat in lactating dairy cows[J].Journal of Dairy Science,2008,91(6):2370-2379.

[24] 齐利枝,闫素梅,生冉,等.奶牛乳腺中乳成分前体物对乳成分合成影响的研究进展[J].动物营养学报,2011,23(12):2077-2083.

[25] 孙满吉,卢德勋,王丽芳,等.基础日粮下关中奶山羊乳腺对营养物质摄取和利用的研究[J].动物营养学报,2009,21(6):859-864.

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