Apparent Digestibility of Crude Protein and Amino Acids of Four Feed Ingredients for Juvenile Soft-Shelled Turtle (Trionyx sinensis)

WU Wenjing; WANG Aimin; GAO Qiang; DOU Chao; LIU Bo; HUANG Jintian; LYU Fu; LIU Wenbin; XU Weina

doi:10.3969/j.issn.1006-267x.2014.12.039

2014 , Vol. 26 >Issue 12: 3843 - 3849

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.12.039

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Apparent Digestibility of Crude Protein and Amino Acids of Four Feed Ingredients for Juvenile Soft-Shelled Turtle (Trionyx sinensis)

WU Wenjing ,
WANG Aimin ,
GAO Qiang ,
DOU Chao ,
LIU Bo ,
HUANG Jintian ,
LYU Fu ,
LIU Wenbin ,
XU Weina

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1. Department of Ocean Technology, Yancheng Institute of Technology, Key Laboratory for Aquaculture and Ecology of Coastal Pool of Jiangsu Province, Yancheng 224051, China;
2. Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China;
3. College of Animal Science and Technology, Nanjing Agricultural Univeristy, Nanjing 210095, China

Received date: 2014-06-09

Online published: 2014-12-05

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Abstract

The apparent digestibility of crude protein and amino acids of 4 feed ingredients—silkworm chrysalis, fermented soybean meal, meat-bone meal and corn gluten meal for juvenile soft-shelled turtle (Trionyx sinensis) were determined in this experiment, in order to explore the new type protein feed ingredients for soft-shelled turtle. Four experimental diets were formulated. The experimental diets were consisted of 70% basal diet and 30% test feed ingredient, and contained 0.5% chromic oxide as an exogenous indicator. One hundred and fifty juvenile soft-shelled turtle with the average body weight of (120.0±1.5) g were randomly divided into 5 groups with 3 replicates per group and 10 soft-shelled turtle per replicate. The soft-shelled turtle in basal diet group were fed the basal diet, and those in the other 4 groups were fed one of experimental diets, respectively. After 1 weeks feeding, the fecal samples were collected by siphon method, and then the apparent digestibility of crude protein and amino acids was determined. The results showed as follows: 1) the apparent digestibility of crude protein in corn gluten meal, fermented soybean meal and silkworm chrysalis for soft-shelled turtle was 90.57%, 86.34% and 85.75%, respectively, higher than that in meat-bone meal (75.98%) (P<0.05). 2) For animal protein sources, the apparent digestibility of total amino acids in silkworm chrysalis was higher, which was 92.44%, while that in meat-bone meal was lower, which only was 75.39%. For plant protein sources, the apparent digestibility of total amino acids in corn gluten meal was higher, which was 96.56%, and that in fermented soybean meal was 87.90%. The results indicate that silkworm chrysalis can be used as a source of high quality animal protein source, and fermented soybean meal can be used as a better plant protein source for soft-shelled turtle.

Key words： soft-shelled turtle (Trionyx sinensis); chromic oxide; crude protein; amino acid; apparent digestibility

Cite this article

WU Wenjing , WANG Aimin , GAO Qiang , DOU Chao , LIU Bo , HUANG Jintian , LYU Fu , LIU Wenbin , XU Weina . Apparent Digestibility of Crude Protein and Amino Acids of Four Feed Ingredients for Juvenile Soft-Shelled Turtle (Trionyx sinensis)[J]. Chinese Journal of Animal Nutrition, 2014 , 26(12) : 3843 -3849 . DOI: 10.3969/j.issn.1006-267x.2014.12.039

References

[1] 郭沛涌,王运涛.水产养殖饲料蛋白源开发利用研究现状[J].中国水产科学,2001,7(4):108-112.

[2] BARROS M M,LIM C,KLESIUS P H.Effect of soybean meal replacement by cottonseed meal and iron supplementation on growth,immune response and resistsmce of channel catfish (Ictalurus puctatus) to Edwardsiella ictaluri challenge[J].Aquaculture,2002,207(3/4):263-279.

[3] BORLONGAN I G,EUSEBI P S,WELSH T.Potential of feed pea (Pisum sativum) meal as a protein source in practical diets for milkfish (Chanos chanos Forsskal)[J].Aquaculture,2003,225(1/2/3/4):89-98.

[4] 姜雪姣,梁丹妮,刘文斌,等.团头鲂对7种饲料的蛋白质、氨基酸及磷的表观消化率[J].中国水产科学,2011,18(1):119-126.

[5] 梁丹妮,姜雪姣,刘文斌,等.建鲤对6种非常规蛋白质原料中营养物质的表观消化率[J].动物营养学报,2011,23(6):1065-1072.

[6] 周凡,丁雪燕,何丰,等.中华鳖日本品系对6种饲料蛋白原料表观消化率的研究[J].水生态学杂志,2014,35(1):81-86.

[7] 何蓉,谢晶,黄硕琳,等.中华鳖在温室养殖、仿生态养殖条件下的营养成分比较研究[J].食品科学,2013,34(13):234-238.

[8] AOAC.Official methods of analytical of the Association of Official Analytical Chemists[S].16th ed.Arlington,VA:AOAC,1995.

[9] FURUKAWA A,TSUKAHARA H.On the acid digestion method for the determination of chromic oxide as an index substance in the study of digestibility of fish feed[J].Bulletin of the Japanese Society of Scientific Fisheries,1966,32:502-508.

[10] 张龙翔.生化实验方法和技术[M].北京:人民教育出版社,1981:94-112.

[11] 徐新章,何珍秀.幼蟹对几种植物性原料氨基酸表观消化吸收率的研究[J].饲料工业,1998,19(2):24-25.

[12] 游文章,雍文岳,廖朝兴,等.测定鱼类饲料原料营养成分消化率的计算方法[J].水产学报,1993,17(2):167-171.

[13] 董晓慧,郭云学,叶继丹,等.吉富罗非鱼幼鱼对10种饲料原料表观消化率的研究[J].动物营养学报,2009,21(3):326-334.

[14] 明建华,叶金云,张易祥,等.2龄青鱼对7种饲料原料中营养物质的表观消化率[J].动物营养学报,2014,26(1):161-169.

[15] AUSTRENG E,STOREBAKKEN T,THOMASSEN M S,et al.Evaluation of selected trivalent metal oxides as inert markers used to estimate apparent digestibility in salmonids[J].Aquaculture,2000,188(1/2):65-78.

[16] 陈建明,叶金云,沈斌乾,等.花鱼骨鱼种对11种蛋白质饲料原料的消化率[J].水生态学杂志,2009,2(3):65-68.

[17] CHO C Y,SlINGER S J.Apparent digestibility measurement in feedstuffs for rainbow trout[M].Berlin:Heenemanm,1979:239-247.

[18] 付晶晶,黄燕华,曹俊明,等.花鲈对6种饲料原料的表观消化率[J].饲料研究,2014,25(3):48-54.

[19] ZHOU Z,REN Z,ZENG H,et al.Apparent digestibility of various feedstuffs for bluntnose black bream Megalobrama amblycephala Yih[J].Aquaculture Nutrition,2008,14(2):153-165.

[20] 刘玉良,朱雅珠,陈慧达.青鱼对十四种饲料的消化率[J].水产科技情报,1990(6):166-169.

[21] 纪文秀,王岩,唐金玉.海水养殖花鲈对几种饲料蛋白原料的表观消化率[J].水产学报,2010,34(1):101-107.

[22] ZOU Y R,AI Q H,MAI K S,et al.Effects of brown fish meal replacement with fermented soybeam meal on growth performance,feed efficiency and enzyme activities of Chinese soft-shelled turtle,Pelodiscus sinensis[J].Journal of Ocean University of China,2012,11(2): 227-235.

[23] 雷武,杨云霞,贺锡勤.长吻鮠对鱼粉等六种商品饲料中粗蛋白和能量的消化率[J].水生生物学报,1996,20(2):113-118.

[24] REGOST C,ARZEL J,KAUSHIK S J.Partial or total replacement of fish meal by corn gluten meal in diet for turbot (Psetta maxima)[J].Aquaculture,1999,180(1/2):99-117.

[25] MASUMOTO T,RUCHIMAT T,ITO Y,et al.Amino acid availability values for several protein sources for yellowtail (Seriola quinqueradiata)[J].Aquaculture,1996,146(1/2):109-119.

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