Swine Nutrition

Effects of Alanyl-Glutamine on the Number of Immunoglobulin A Plasma Cells in Intestinal Lamina Propria and the Contents of Secretory Immunoglobulin A and Interleukin in Mucosal of Weaner Piglets

  • YE Yaling ,
  • WANG Zirui ,
  • YOU Jinming ,
  • DENG Chenxi ,
  • XIN Xiangrong
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  • Key Lab of Animal Science of Jiangxi Province, Jiangxi Agricultural University, Nanchang 330045, China

Received date: 2014-07-18

  Online published: 2015-01-10

Abstract

The study was conducted to study the effects of alanyl-glutamine (Ala-Gln) on the number of Immunoglobulin A (IgA) plasma cells in intestinal lamina propria and the contents of secretory immunoglobulin A (SIgA) and interleukin (IL) in mucosal of weaner piglets. The experiment used a randomized complete block design, a total of 100 castrated weaner piglets (Landrace ×Large White ) at (21±2) days of age with initial body weight of (6.7±0.9) kg were assigned to four groups with five replicates in each group and five piglets in each replicate. Piglets in the control group were fed a basal diet (free alanyl-glutamine), and the others were fed the basal diet supplemented with 0.15%, 0.30% and 0.45% Ala-Gln, respectively. The experiment lasted for 21 days. The results showed as follows: 1) compared with the control group, dietary supplemented with 0.15% to 0.45% Ala-Gln significantly elevated the number of IgA plasma cells in duodenum and jejunum lamina propria on 7, 14 and 21 days of post-weaning (P<0.05), especially, the number of IgA plasma cells in jejunum of 0.30% Ala-Gln group was increased by 22.6% on 14 days of post-weaning (P<0.05); in ileum, the number of IgA plasma cells of 0.30% Ala-Gln group was significantly increased on 7 and 14 days of post-weaning (P<0.05). 2) With the increasing of Ala-Gln addition in diet, the content of SIgA in jejunal mucosal quadratically increased. Compared with the control group, the SIgA content of 0.30% Ala-Gln group was increased by 70.4% on 7 days of post-weaning (P<0.01), both 0.15% and 0.30% Ala-Gln groups was significantly increased on 14 and 21 days of post-weaning (P<0.05). 3) Compared with the control group, dietary supplemented with 0.15% and 0.30% Ala-Gln significantly increased the contents of IL-2 and IL-5 on on 7 days of post-weaning (P<0.05), and also significantly increased the contents of IL-5, IL-2 on 14 days of post-weaning (P<0.05), respectively. The other Ala-Gln addition had no significantly difference (P>0.05). With the increasing of Ala-Gln addition in diet, the contents of IL-6, IL-10 showed a linear increasing trend, and was markedly higher than the control group (P<0.05). In conclusion, dietary supplemented with Ala-Gln can elevate the number of IgA plasma cells and the content of SIgA and interleukin in intestinal mucosa, and improve the intestinal mucosa cellular immunity of weaner piglets.

Cite this article

YE Yaling , WANG Zirui , YOU Jinming , DENG Chenxi , XIN Xiangrong . Effects of Alanyl-Glutamine on the Number of Immunoglobulin A Plasma Cells in Intestinal Lamina Propria and the Contents of Secretory Immunoglobulin A and Interleukin in Mucosal of Weaner Piglets[J]. Chinese Journal of Animal Nutrition, 2015 , 27(1) : 59 -66 . DOI: 10.3969/j.issn.1006-267x.2015.01.009

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