Effects of C3 and C4 Plants of Roughage and Concentrate to Roughage Ratio of Substrate on in Vitro Ruminal Fermentation Characteristics of Goats

CHEN An; WU Jianping; ZHOU Chuanshe; TAN Zhiliang

doi:10.3969/j.issn.1006-267x.2015.01.015

2015 , Vol. 27 >Issue 1: 112 - 123

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.01.015

Ruminant Nutrition

Effects of C3 and C4 Plants of Roughage and Concentrate to Roughage Ratio of Substrate on in Vitro Ruminal Fermentation Characteristics of Goats

CHEN An ,
WU Jianping ,
ZHOU Chuanshe ,
TAN Zhiliang

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1. Gansu Agricultural University, Lanzhou 730070, China;
2. Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan Research Center of Livestock & Poultry Sciences, and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha, Hunan 410125, China

Received date: 2014-07-08

Online published: 2015-01-10

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Abstract

This experiment was designed to study the effects of C3 and C4 plants of roughage and concentrate to roughage ratio (C: R) of substrate on in vitro ruminal fermentation characteristics of goats. The study was conducted in a completely randomized block design of experiment, different sources of C3 (rice straw and alfalfa) and C4 plants (maize stover and broadleaf paspalum) were selected as roughages, and three C: Rs (40: 60, 45: 55 and 50: 50) were set for each fermentation substrates, respectively. By employing the method of in vitro ruminal fermentation (48 hours), gas production parameters, fermentation characteristic parameters and nutrient degradability were measured. The results showed as follows: 1) using alfalfa as roughage of substrate, theological maximum gas production was significantly higher than the other three roughages (P<0.01), and it was higher when C: Rs were 45: 55 and 50: 50; using rice straw as roughage of substrate, methane production was decreased by 53.79%, 24.30% and 3.29%, respectively, compared with alfalfa, maize stover and broadleaf paspalum, and it was lowest when C: R was 45: 55. 2) The pH and NH₃-N concentration of fermentation fluid of C3 plants (rice straw and alfalfa) was significantly higher than those of C4 plants (maize stover and broadleaf paspalum) (P<0.01), but C: R did not significantly affect those two indices (P>0.05). 3) Using rice straw as roughage of substrate, the concentrations of acetic acid, propionic acid and total volatile fatty acid was significantly higher than those of maize stover and broadleaf paspalum (P<0.01), while there were no significant differences between rice straw and alfalfa (P>0.05); acetic acid/propionic acid of rice straw and maize stover for C: R at 50: 50 was significantly higher than that at 40: 60 (P<0.05), while there were no significant differences between C: Rs of 50: 50 and 45: 55 (P>0.05). 4) in vitro dry matter degradability and in vitro neutral detergent fiber degradability of substrates were the lowest when using rice straw as roughage, and were decreased by 18.84%, 4.37% and 3.84%, and 11.12%, 17.78% and 15.56%, respectively, compared with those of alfalfa, maize stover and broadleaf paspalum. It is concluded that when alfalfa is selected as roughage and C: R is 50: 50, the in vitro ruminal degradability and methane production are both the highest. However, when rice straw is selected as roughage and C: R is 45: 55, the in vitro ruminal degradability and methane production are both the lowest.

Key words： C3 plant; C4 plant; concentrate to roughage ratio; in vitro fermentation; goat

Cite this article

CHEN An , WU Jianping , ZHOU Chuanshe , TAN Zhiliang . Effects of C3 and C4 Plants of Roughage and Concentrate to Roughage Ratio of Substrate on in Vitro Ruminal Fermentation Characteristics of Goats[J]. Chinese Journal of Animal Nutrition, 2015 , 27(1) : 112 -123 . DOI: 10.3969/j.issn.1006-267x.2015.01.015

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