Effects of Different Protein Ratios of Fish Meal to Fermented and Enzymolysis Soybean Meal on Growth and Immune Performance of Oriental River Prawn (Macrobrachium nipponense)

DING Zhili; ZHANG Yixiang; YE Jinyun; ZHOU Zhijin; DU Zhenyu

doi:10.3969/j.issn.1006-267x.2015.01.019

2015 , Vol. 27 >Issue 1: 154 - 164

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.01.019

Aquaculture Nutrition

Effects of Different Protein Ratios of Fish Meal to Fermented and Enzymolysis Soybean Meal on Growth and Immune Performance of Oriental River Prawn (Macrobrachium nipponense)

DING Zhili ,
ZHANG Yixiang ,
YE Jinyun ,
ZHOU Zhijin ,
DU Zhenyu

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1. Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition, CAFS, College of Life Sciences, Huzhou University, Huzhou 313000, China;
2. Huzhou Municipal Fisheries Extension Center, Huzhou 313000, China;
3. School of Life Science, East China Normal University, Shanghai 200062, China

Received date: 2014-08-13

Online published: 2015-01-10

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Abstract

This experiment was conducted to evalute the effects of different protein ratios of fish meal to fermented and enzymolysis soybean meal with microencapsulated amino acid supplementation in diets on growth and immune performance of oriental river prawn (Macrobrachium nipponense). Fermented enzymolysis soybean meal replaced 0 (FM group), 25% (R25 group), 50% (R50 group), 75% (R75 group) and 100% of fish meal (R100 group) (the protein ratios of fish meal to fermented and enzymolysis soybean meal were 1: 0, 3: 1, 1: 1, 1: 3 and 0: 1, respectively) in five isocaloric and isonitrogenous diets, respectively. The oriental river prawn with the average body weight of (0.103 0±0.000 2) g were randomly divided into 5 groups with 5 replicates in each group and 50 prawns in each replicate. The experiment lasted for 8 weeks. After the feeding experiment, the prawns were challenged with Aeromonas hydrophila. The results showed that weight gain rate and specific growth rate were the highest when the protein ratio of fish meal to fermented and enzymolysis soybean meal was 1: 1, and were significantly higher than those in the FM and R100 groups (P<0.05), while no significant difference was observed in survival ratio among all groups (P>0.05). There were no significant differences in the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) in hepatopancreas among all groups (P>0.05). The total haemocyte count (THC) and haemolymph phagocytic activity in the FM group were significantly higher than those in the R100 group (P<0.05). The hepatopancreas heat shock cognate protein (HSC70) mRNA relative expression level in the FM group was the highest, and significantly higher than that in the other groups (P<0.05), the same of heat shock protein 90 (HSP90) in R25 group (P<0.05). Additionally, the cumulative mortality was significantly increased when the ratio of fish meal replacement with fermented and enzymolysis soybean meal was more than 50% after challenged with Aeromonas hydrophila. Therefore, fermented and enzymolysis soybean meal can be an appropriate protein source in the diet of oriental river prawn, and the optimal dietary protein ratio of fish meal to fermented and enzymolysis soybean meal for oriental river prawn is 1: 1 under the condition of amino acid balance.

Key words： oriental river prawn (Macrobrachium nipponense); fermented and enzymolysis soybean meal; fish meal; growth performance; immunity

Cite this article

DING Zhili , ZHANG Yixiang , YE Jinyun , ZHOU Zhijin , DU Zhenyu . Effects of Different Protein Ratios of Fish Meal to Fermented and Enzymolysis Soybean Meal on Growth and Immune Performance of Oriental River Prawn (Macrobrachium nipponense)[J]. Chinese Journal of Animal Nutrition, 2015 , 27(1) : 154 -164 . DOI: 10.3969/j.issn.1006-267x.2015.01.019

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