Experimental Methods

Analysis of Variation of Genome DNA Methylation Level of Chick Embryo Tissues with High Performance Liquid Chromatography

  • LI Shizhao ,
  • GUO Wei ,
  • DU Chao ,
  • JING Yufei ,
  • LIU Yanli ,
  • SHEN Jing ,
  • YAO Junhu ,
  • YANG Xiaojun
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  • College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China

Received date: 2014-08-24

  Online published: 2015-01-10

Abstract

This experiment was conducted to develop the high performance liquid chromatography (HPLC) method for genome DNA methylation level detection, and to explore the variation of genome DNA methylation level of chick embryo tissues preliminary. Firstly, the genome DNA methylation level detection method was improved from 2 aspects including DNA enzymatic hydrolysis system and HPLC instrument detection condition; then, the DNA methylation level of heart, liver and muscle at embryonic ages of 8, 11, 14, 17 days of Cobb 500 broiler chick embryo was detected by improved HPLC method, respectively. The results showed that the genome DNA methylation level of 3 tissues was increased with embryonic age increasing and that of liver was significantly higher than that of heart and muscle in later embryonic development (embryonic age of 17 days) (P<0.05). Heart and muscle showed no significant difference in DNA methylation level among all embryonic ages (P>0.05). It is concluded that the developed HPLC laid a good foundation for genome DNA methylation determination of tissues rapidly and accurately. Otherwise, the genome DNA methylation level rises gradually with chick embryonic age increasing, and that of liver is higher in later embryonic development.

Key words: HPLC; chick; embryo; DNA methylation

Cite this article

LI Shizhao , GUO Wei , DU Chao , JING Yufei , LIU Yanli , SHEN Jing , YAO Junhu , YANG Xiaojun . Analysis of Variation of Genome DNA Methylation Level of Chick Embryo Tissues with High Performance Liquid Chromatography[J]. Chinese Journal of Animal Nutrition, 2015 , 27(1) : 171 -177 . DOI: 10.3969/j.issn.1006-267x.2015.01.021

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