Effects of &beta;-Hydroxybutyric Acid on Relative Expression Levels of Genes Related to Milk Fat Synthesis in Bovine Mammary Epithelial Cells

CHANG Chencheng; QI Lizhi; YAN Sumei; SHENG Ran; ZHAO Yanli

doi:10.3969/j.issn.1006-267x.2015.01.024

2015 , Vol. 27 >Issue 1: 196 - 203

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.01.024

Feed Science and Technology

Effects of β-Hydroxybutyric Acid on Relative Expression Levels of Genes Related to Milk Fat Synthesis in Bovine Mammary Epithelial Cells

CHANG Chencheng ,
QI Lizhi ,
YAN Sumei ,
SHENG Ran ,
ZHAO Yanli

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College of Animal Science, Inner Mongolia Agricultural University, Huhhot 010018, China

Received date: 2014-07-11

Online published: 2015-01-10

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Abstract

This study was conducted to determine the effects of β-hydroxybutyric acid (BHBA) on cell viability, triacylglycerol (TAG) content, lipid droplet formation and relative expression levels of genes related to milk fat synthesis in bovine mammary epithelial cells (BMECs). The 3th passage cells were plated 1×10⁵ cells/well in culture plates and DMEM/F12 medium containing 10% fetal bovine serum (FBS) was added to each well, then the plates were maintained in an incubator with 5% CO₂ at 37 ℃. After 48 h, cells were randomly divided into 6 groups and cultured in DMEM/F12 medium containing different concentrations of BHBA, FBS in medium was replaced with 1 g/L fatty acid-free bovine serum albumin (BSA). The final concentrations of BHBA in reaction systems were 0 (control), 0.58, 1.16, 2.32, 4.64, 9.28 mmol/L, respectively. Continuous incubation in an incubator with 5% CO₂ at 37 ℃ for 48 h. The results showed that BMECs proliferation [relative growth rate (RGR)] was significantly quadratic increased with BHBA concentration increasing (P=0.041), and BMECs proliferation of 0.58 to 4.64 mmol/L BHAB groups was high, but 9.28 mmol/L BHBA group was low. BHBA at low concentration (0.58 to 2.32 mmol/L) promoted lipid droplet formation, and the promoting effect of BHBA at high concentration (4.64 to 9.28 mmol/L) was weakened. No significant linear and quadratic relationships were found between BHBA concentration and TAG content (P>0.05). No significant linear and quadratic relationships were found between BHBA concentration and relative expression levels of genes related to milk fat synthesis (fatty acid synthetase, acetyl-CoA carboxylase α, stearyl coenzyme A desaturase, fatty acid binding protein 3, peroxisome proliferator-activated receptor γ and cluster of differentiation 36) (P>0.05). In conclusion, BMECs proliferation significantly quadratic increase with BHBA concentration increasing, namely BMECs proliferation has significant concentration-dependent relationship with BHBA concentration, and the milk fat synthesis has a improve trend by BHBA concentration.

Key words： β-hydroxybutyric acid; mammary epithelial cells; genes related to milk fat synthesis; dairy cows

Cite this article

CHANG Chencheng , QI Lizhi , YAN Sumei , SHENG Ran , ZHAO Yanli . Effects of β-Hydroxybutyric Acid on Relative Expression Levels of Genes Related to Milk Fat Synthesis in Bovine Mammary Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2015 , 27(1) : 196 -203 . DOI: 10.3969/j.issn.1006-267x.2015.01.024

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