Effects of T-2 Toxin on Growth Performance, Blood Indexes and Antioxidant Ability in BALB/c Mice

CHEN Huiying; YANG Junhua; LUO Aiqiong; GUO Wenbo; HAN Wei; ZHAO Zhihui

doi:10.3969/j.issn.1006-267x.2015.01.026

Chinese Journal of Animal Nutrition >

2015 , Vol. 27 >Issue 1: 212 - 220

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.01.026

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Effects of T-2 Toxin on Growth Performance, Blood Indexes and Antioxidant Ability in BALB/c Mice

CHEN Huiying ,
YANG Junhua ,
LUO Aiqiong ,
GUO Wenbo ,
HAN Wei ,
ZHAO Zhihui

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1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;
2. Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China

Received date: 2014-07-24

Online published: 2015-01-10

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Abstract

This study was conducted to investigate the effects of T-2 toxin on growth performance, blood routine examination, serum biochemical indexes and antioxidant ability of liver in mice. A total of 80 BALB/c mice weighted about 20 g were randomly allotted to 4 groups with 20 replicates per group and 1 mouse per replicate. The mice were fed one of the 4 kinds of diets [0 (control), 0.4, 1.0, 2.5 mg/kg BW T-2 toxin exposed daily via intragastric administration] for 28 days. The results showed as follows: in 2.5 mg/kg BW group, the morality of mice was close to 25%, and average daily gain and average daily feed intake were significantly decreased (P<0.01). However, the organ coefficients of heart, liver, spleen, lung, kidney and brain in 2.5 mg/kg BW group were increased markedly (P<0.05 or P<0.01), but those of testis, seminal vesicle, uterus and ovaries were reduced significantly (P<0.05 or P<0.01). Compared with control group, organ coefficient of liver and the counts of white blood cell (WBC), lymphocyte (LY), monocytes (MO), neutrophil granulocyte (GR) were significantly increased in 0.4 and 1.0 mg/kg BW groups (P<0.05 or P<0.01), respectively. Additionally, compared with control group, the counts of MO, GR and platelet (PLT) in 2.5 mg/kg BW group were significantly increased (P<0.01), however, red blood count (RBC) and hemoglobin (HGB) content were significantly reduced (P<0.05 or P<0.01), respectively. Furthermore, in serum biochemical indexes, the contents of glucose (GLU), urea and creatinine (CRE), and the activities of alkaline phosphatase (ALP) and alanine aminotransferase (ALT) had improved trends with the increase of T-2 toxin dose, but the contents of triglyceride (TG) and albumin (ALB) had reduced trends, and those were significantly different with those of control group (P<0.05 or P<0.01). In addition, with the increase of T-2 toxin dose, the content of malondialdehyde (MDA) in liver tissue was also advanced, then the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) had decreased trends. It is concluded that the supplementation of T-2 toxin under 1.0 mg/kg BW can induce the immunostimulation. Nevertheless, the excessive dose can cause the inhibition of growth performance, induce organ damage of liver and kidney, and reduce the antioxidant ability of liver significantly with dose-effect relationship.

Key words： T-2 toxin; growth performance; blood routine examination; serum biochemical indexes; antioxidant

Cite this article

CHEN Huiying , YANG Junhua , LUO Aiqiong , GUO Wenbo , HAN Wei , ZHAO Zhihui . Effects of T-2 Toxin on Growth Performance, Blood Indexes and Antioxidant Ability in BALB/c Mice[J]. Chinese Journal of Animal Nutrition, 2015 , 27(1) : 212 -220 . DOI: 10.3969/j.issn.1006-267x.2015.01.026

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