Effects of Lactobacillus delbrueckii on Performance, Serum Lipid Indexes, Total Cholesterol and Total Bile Acid Contents in Feces and Tissues of Fattening Pigs

LI Rui; HOU Gaifeng; LIU Ming; PENG Wei; HUANG Xingguo

doi:10.3969/j.issn.1006-267x.2015.01.030

2015 , Vol. 27 >Issue 1: 247 - 255

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.01.030

Short Communications

Effects of Lactobacillus delbrueckii on Performance, Serum Lipid Indexes, Total Cholesterol and Total Bile Acid Contents in Feces and Tissues of Fattening Pigs

LI Rui ,
HOU Gaifeng ,
LIU Ming ,
PENG Wei ,
HUANG Xingguo

Expand

1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Received date: 2014-07-18

Online published: 2015-01-10

Fold

Abstract

This experiment was conducted to study the effects of Lactobacillus delbrueckii on performance, serum lipid indexes, total cholesterol and total bile acid contents in feces and tissues of fattening pigs. A total of 120 healthy crossbred Duroc×(Landrace×Yorkshire) fattening pigs with the average body weight of (65.34±3.64) kg were randomly divided into 2 groups with 6 replicates per group and 10 pigs per replicate (half male and half female). The control group was fed a basal diet without antibiotics, while the experimental group was fed the basal diet added with 0.1% Lactobacillus delbrueckii. The pre-test lasted for 7 days, and the experiment lasted for 42 days. On days 14, 28 and 42 of experiment, one pig (each group included 3 male and 3 female pigs) was selected from each replicate to collect anterior vena cava blood, and in the period of day 12 to day 14, day 26 to day 28 and day 40 to day 42, fresh feces of each replicate was collected. At the end of experiment, one pig of each replicate whose weight was closed to the average body weight was selected to slaughter for isolating liver, longissimus muscle and myocardium. The results showed as follows: 1) compared with the control group, there was no significant difference in average daily gain (ADG), average daily feed intake (ADFI) and the ratio of feed to gain (F/G) (P>0.05), but the diarrhea rate and morbidity were reduced extremely significantly or significantly in the experimental group (P<0.01 or P<0.05). 2) There was no significant difference in the contents of serum total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol (LDL-C), high density lipoprotein-cholesterol (HDH-C) and the ratio of HDL-C to LDL-C between the 2 groups on days 14, 28 and 42 (P>0.05), but the content of serum total bile acid was decreased significantly on days 14 and 42 (P<0.05). 3) During the period of day 12 to day 14, compared with the control group, the fecal TBA content in the experimental group was significantly increased (P<0.05), but the fecal TC content was extremely significantly decreased (P<0.01); from day 26 to day 28, the contents of fecal TBA and TC in the experimental group were not significantly different from those in the control group (P>0.05); from day 40 to day 42, the fecal TBA content in the experimental group was significantly higher than that in the control group (P<0.05), but there was no significant difference in the fecal TC content between the 2 groups (P>0.05). 4) Compared with the control group, the TC content in liver, myocardium and longissimus muscle and the TBA content in liver in the experimental group had no significant differences (P>0.05). In conclusion, these results indicate that diet added with 0.1% Lactobacillus delbrueckii can improve the performance and serum lipid status, promote the fecal cholesterol and bile acid excretion of fattening pigs.

Key words： Lactobacillus delbrueckii; fattening pigs; serum lipid indexes; cholesterol; bile acid

Cite this article

LI Rui , HOU Gaifeng , LIU Ming , PENG Wei , HUANG Xingguo . Effects of Lactobacillus delbrueckii on Performance, Serum Lipid Indexes, Total Cholesterol and Total Bile Acid Contents in Feces and Tissues of Fattening Pigs[J]. Chinese Journal of Animal Nutrition, 2015 , 27(1) : 247 -255 . DOI: 10.3969/j.issn.1006-267x.2015.01.030

References

[1] MAXFIED F R,TABAS I.Role of cholesterol and lipid organization in disease[J].Nature,2005,438(7068):612-621.

[2] ABD EI-GAEAD I A,EI-SAYED E M,HAFEZ S A,et al.The hypocholesterolaemic effect of milk yoghurt and soy-yoghurt containing Bifidobacteria in rats fed on a cholesterol-enriched diet[J].International Dairy Journal,2005,15(1):37-44.

[3] CAVALLINI D C U,BEDANI R,BOMDESPACHO L Q,et al.Effects of probiotic bacteria,isoflavones and simvastatin on lipid profile and atherosclerosis in cholesterol-fed rabbits:a randomized double-blind study[J].Lipids in Health and Disease,2009,8:1.

[4] WHO.Cardiovascular disease [EB/OL].[2010-05-19]http://www.who.int/mediacentre/factsheets/fs317/en/print.html.

[5] LYE H S,RAHMAT-ALI G R,LIONG M T.Mechanisms of cholesterol removal by Lactobacilli under conditions that mimic the human gastrointestinal tract[J].International Dairy Journal,2010,20(3):169-175.

[6] HUANG Y,WANG X J,WANG J F,et al.Lactobacillus plantarum strains as potential probiotic cultures with cholesterol-lowering activity[J].Journal of Dairy Science,2013,96(5):2746-2753.

[7] 王今雨,满朝新,杨相宜,等.植物乳杆菌NDC75017的降胆固醇作用[J].食品科学,2013,34(3):243-247.

[8] SOHAIL M U,JAS A I,YOUSAF M S,et al.Alleviation of cyclic heat stress in broilers by dietary supplementation of mannan-oligosaccharide and Lactobacillus-based probiotic:dynamics of cortisol,thyroid hormones,cholesterol,C-reactive protein,and humoral immunity[J].Poultry Science,2010,89(9):1934-1938.

[9] MIKULSKI D,JANKOWSKI J,NACZMANSKI J,et al.Effects of dietary probiotic (Pediococcus acidilactici) supplementation on performance,nutrient digestibility,egg traits,egg yolk cholesterol,and fatty acid profile in laying hens[J].Poultry Science,2012,91(10):2691-2700.

[10] 李瑞,侯改凤,黄其永,等.德氏乳杆菌对哺乳仔猪生长性能、血清生化指标、免疫和抗氧化功能的影响[J].动物营养学报,2013,25(12):2943-2950.

[11] MEUNIER A,LALANNE F,NICOLLE C,et al.Blood-cholesterol-lowering strain of Lactobacillus delbrueckii[P].US:08652461,2014-02-18.

[12] KIM M,SHIN H K.The water soluble extract of chicory influences serum and liver lipid concentrations,cecal short-chain fatty acid concentrations and fecal lipid excretion in rats[J].The Journal of Nutrition,1998,128(10):1731-1736.

[13] 孙建广,张石蕊,谯仕彦,等.发酵乳酸杆菌对生长肥育猪生长性能和肉品质的影响[J].动物营养学报,2010,22(1):132-138.

[14] GIANG H H,VIET T Q,OGLE B,et al.Effects of supplementation of probiotics on the performance,nutrient digestibility and faecal microflora in growing-finishing pigs[J].Asian-Australasian Journal of Animal Sciences,2011,24(5):655-661.

[15] 王俊,安莉.乳酸菌发酵饲料对猪生长性能和猪舍环境的影响[J].饲料工业,2012,33(10):60-62.

[16] BON M L,DAVIES H E,GLYNN C,et al.Influence of probiotics on gut health in the weaned pig[J].Livestock Science,2010,133(1/2/3):179-181.

[17] SIEO C C,ABDULLAH N,TAN W S,et al.Influence of β-glucanase producing Lactobacillus strains on intestinal characteristics and feed passage rate of broiler chickens[J].Poultry Science,2005,84 (5):734-741.

[18] SERVIN A L.Antagonistic activities of Lactobacilli and Bifidobacteria against microbial pathogens[J].FEMS Microbiology Reviews,2004,28(4):405-440.

[19] VOLTAN S,MARTINES D,ELLI M,et al.Lactobacillus crispatus M247-derived H₂O₂ acts as a signal transducing molecule activating peroxisome proliferator activated receptor-γ in the intestinal mucosa[J].Gastroenterology,2008,135(4):1216-1227.

[20] TOBA T,YOSHIOKA E,ITOH T.Lacticin,a bacteriocin produced by Lactobacillus delbrueckii subsp.Lactis[J].Letters in Applied Microbiology,1991,12(2):43-45.

[21] DE RODAS B Z,GILLILAND S E,MAXWELL C V.Hypocholesterolemic action of Lactobacillus acidophilus ATCC 43121 and calcium in swine with hypercholesterolemia induced by diet[J].Journal of Dairy Science,1996,79(12):2121-2128.

[22] 杨晶,杨庆.具有降胆固醇作用乳酸菌的研究和开发进展[J].中国微生物学杂志,2011,23(11):1047-1049.

[23] LIONG M T,DUNSHEA F R,SHAH N P.Effects of a synbiotic containing Lactobacillus acidophilus ATCC 4962 on plasma lipid profiles and morphology of erythrocytes in hypercholesterolaemic pigs on high- and low-fat diets[J].British Journal of Nutrition,2007,98(4):736-744.

[24] 廖端芳,唐朝克.胆固醇逆向转运基础与临床[M].北京:科学出版社,2009.

[25] 邹思湘.动物生物化学[M].4版.北京:中国农业出版社,2007.

[26] MAJEED F,MILLER M.Low high density lipoprotein cholesterol:an important consideration in coronary heart disease risk assessment[J].Current Opinion in Endocrinology Diabetes and Obesity,2008,15(2):175-181.

[27] 张明明.高胆固醇血症低密度脂蛋白受体基因突变的研究进展[J].河北医科大学学报,2011,32(7):866-868.

[28] 于平,孙海森,励建荣,等.植物乳杆菌LpT1和LpT2体外降胆固醇机制[J].微生物学报,2011,51(4):561-565.

[29] LYE H S,RUSUL G,LIONG M T.Removal of cholesterol by Lactobacilli via incorporation of and conversion to coprostanol[J].Journal of Dairy Science,2010,93(4):1383-1392.

[30] 王俊国,孟和毕力格,包秋华,等.具有胆盐水解酶活性的乳酸菌对高血脂症大鼠血脂的调节作用[J].食品科学,2013,34(1):257-262.

[31] 许女,王艳萍,习傲登,等.植物乳杆菌的降胆固研究[J].中国食品学报,2012,12(4):30-35.

[32] 于志会,张雪,李盛钰,等.植物乳杆菌C88发酵乳对高脂血症小鼠降胆固醇的作用[J].中国乳品工业,2013,41(8):17-19.

[33] HIGURASHI S,KUNIEDA Y,MATSUYAMA H,et al.Effect of cheese consumption on the accumulation of abdominal adipose and decrease in serum adiponectin levels in rats fed a calorie dense diet[J].International Dairy Journal,2007,17(10):1224-1231.

[34] 徐晶雪,邹积宏,袁杰利,等.乳酸菌对高脂小鼠降胆固醇作用的研究[J].中国微生态学杂志,2011,23(1):5-7.

[35] 王巍,邹积宏,袁杰利,等.降胆固醇乳酸菌对肉鸡胆固醇的影响研究[J].中国微生物学杂志,2009,21(3):200-204.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References