Effects of Dietary Rumen Degradable Protein Level on Microbial Nitrogen Yield and Nitrogen Retention of Finishing Kazakh Sheep

GAO Yang; GAO Wei; CHEN Teng; ZHANG Mei; NI Chuanwu; LIU Chenli; CHEN Daofu

doi:10.3969/j.issn.1006-267x.2015.01.033

2015 , Vol. 27 >Issue 1: 274 - 280

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.01.033

Short Communications

Effects of Dietary Rumen Degradable Protein Level on Microbial Nitrogen Yield and Nitrogen Retention of Finishing Kazakh Sheep

GAO Yang ,
GAO Wei ,
CHEN Teng ,
ZHANG Mei ,
NI Chuanwu ,
LIU Chenli ,
CHEN Daofu

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College of Animal Science & Technology, Shihezi University, Shihezi 832003, China

Received date: 2014-07-29

Online published: 2015-01-10

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Abstract

The aim of this study was to investigate the effects of increased dietary rumen degradable protein (RDP) level on rumen microbial nitrogen (MN) yield and nitrogen retention of finishing Kazakh sheep under inferior forage feeding condition. Three lambs weighed (30.0±2.8) kg were fed three diets with different RDP levels [7.94%(TMR1), 9.02%(TMR2) and 10.10% (TMR3)]. A 3×3 Latin square experimental design was adopted. There were three periods with 10-day pre-test period and 5-day test period in each period. Total collection of feces and urine method was used to determine urinary purine derivatives (PD) excretion, rumen MN yield and conversion rate. The results showed as follows: the intake and apparent digestibility of dry matter (DM) and organic matter (OM) of the three diets were not significantly different (P>0.05), however, nitrogen retention, urinary PD excretion as well as rumen MN yield of TMR3 were significantly higher than those in TMR1 and TMR2 (P<0.05). There was a significant positive relation between dietary RDP level and MN yield/digestible OM intake (R²=0.999 4,P=0.010 9). The conversion rate of MCP was not affected by dietary RDP level (P>0.05). The results indicate that under inferior forage feeding condition, when dietary RDP level is increased from 7.94% to 10.10%, nitrogen retention and rumen microbial protein (MCP) synthesis can be improved, while the conversion rate of MCP is not affected.

Key words： purine derivatives; microbial nitrogen; rumen degradation protein; nitrogen retention; Kazakh sheep

Cite this article

GAO Yang , GAO Wei , CHEN Teng , ZHANG Mei , NI Chuanwu , LIU Chenli , CHEN Daofu . Effects of Dietary Rumen Degradable Protein Level on Microbial Nitrogen Yield and Nitrogen Retention of Finishing Kazakh Sheep[J]. Chinese Journal of Animal Nutrition, 2015 , 27(1) : 274 -280 . DOI: 10.3969/j.issn.1006-267x.2015.01.033

References

[1] TOPPS J H,ELLIOTT R C.Relationship between concentrations of ruminal nucleic acids and excretion of purine derivatives by sheep[J].Nature,1965,205(4970):498-499.

[2] CHEN X B,GOMES M J.Estimation of microbial protein supply to sheep and cattle based on urinary excretion of purine derivatives-an overview of the technical details[R].Bucksburn:Rowett Research Institute,1995:1-21.

[3] IAEA.Estimation of rumen microbial protein production from purine derivatives in urine.A laboratory manual for FAO/IAEA co-ordinated research programme on development,standardization and validation of nuclear based technologies for measuring microbial protein supply in ruminant livestock for improving productivity.IAEA-TECDOC-945[S].Vienna:IAEA,1997:7-11.

[4] 徐萍,莫放,姜军,等.中国荷斯坦成年公牛可消化有机物进食量与尿嘌呤衍生物排出量的关系分析[J].中国农学通报,2007,23(2):1-4.

[5] BECKERS Y,THÉWIS A,SOHY C,et al.Measurements of microbial N flow to the duodenum and urinary excretion of purine derivatives in bulls[J].Annales de Zootechnie,1995,44(Suppl.1):177.

[6] CARRO M D,VALDES C,RANILLA M J.Effect of feed intake level on duodenal flow of microbial nitrogen in sheep given complete diets[J].ITEA Production Animal,1999,20(2):490-492.

[7] CHEN X B,CHEN Y K,FRANKLIN M F,et al.The effect of feed intake and body weight on purine derivative excretion and microbial protein supply in sheep[J].Journal of Animal Science,1992,70(5):1534-1542.

[8] DIPU M T,GEORGE S K,SHINGH P,et al.Measurement of microbial protein supply in Murrah Buffaloes (Bubalus bubalis) using urinary purine derivatives excretion and PDC index[J].Asian-Australasian Journal of Animal Sciences,2006,19(3):347-355.

[9] PÉREZ J F,BALCELLS J,CEBRIÁN J A,et al.Excretion of endogenous and exogenous purine derivatives in sheep:effect of increased concentrate intake[J].British Journal of Nutrition,1998,79(3):237-240.

[10] 钟伟,李光玉,罗国良,等.限饲对东北梅花鹿消化代谢及尿嘌呤衍生物排出量的影响[J].饲料工业,2009,30(21):44-47.

[11] 邢壮.不同日粮类型对肉牛尿中嘌呤衍生物排出量的影响[D].硕士学位论文.北京:中国农业大学,2001:19-42.

[12] MARTÍN-ORÙE S M,BALCELLS J,GUADA J A,et al.Microbial nitrogen production in growing heifers:direct measurement of duodenal flow of purine bases versus urinary excretion of purine derivatives as estimation procedures[J].Animal Feed Science and Technology,2000,88(3/4):171-188.

[13] ØRDKOV E R,MCDONALD I.The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage[J].The Journal of Agricultural Science,1979,92:499-503.

[14] 张丽英.饲料分析及饲料质量检测技术[M].北京:中国农业大学出版社,2003.

[15] 倪传武,高巍,高杨,等.育肥羊全混合日粮的瘤胃有效降解率及能氮平衡研究[J].饲料研究,2014,9:1-3.

[16] SAS.SAS user's guide:statistics[M].Cary:SAS Inst.,Inc.,2000.

[17] NRC.Nutrient requirements of dairy cattle[S].7th ed.Washington,D.C.:National Academy Press,2001.

[18] LIANG J B,MATSUMOTO M,YOUNG B A.Purine derivative excretion and ruminal microbial yield in Malaysian cattle and swamp buffalo[J].Animal Feed Science and Technology,1994,47(3/4):189-199.

[19] CHEN X B,SAMARAWEERA L,KYLE D J,et al.Urinary excretion of purine derivatives and tissue xanthine oxidase (EC 1.2.3.2) activity in buffaloes (Bubalis bubalis),with special reference to differences between buffaloes and Bos taurus cattle[J].British Journal of Nutrition,1996,75(3):397-407.

[20] LONG R J,DONG S K,CHEN X B,et al.Preliminary studies on urinary excretion of purine derivatives and creatinine in yaks[J].The Journal of Agricultural Science,1999,133(4):427-431.

[21] PÉREZ J F,BALCELLS J,GUADA J A,et al.Rumen microbial production estimated either from urinary purine derivative excretion or from direct measurements of ¹⁵N and purine bases as microbial markers:effect of protein source and rumen bacteria isolates[J].Animal Science,1997,65(2):225-236.

[22] 马涛,刁其玉,邓凯东,等.日粮不同精粗比对肉羊氮沉积和尿嘌呤衍生物排出量的影响[J].中国畜牧杂志,2012,48(15):29-33.

[23] LINDBERG J E,JACOBSSON K G.Nitrogen and purine metabolism at varying energy and protein supplies in sheep sustained on intragastric infusion[J].British Journal of Nutrition,1990,64(2):359-370.

[24] MAKKAR H P S.Development,standardization and validation of nuclear based technologies for estimating microbial protein supply in ruminant livestock for improving productivity[M]//Estimation of microbial protein supply in ruminants using urinary purine derivatives.Netherlands:Springer,2004:1-13.

[25] 黄鸿威,莫放,周汉林.不同营养水平对4-6月龄荷斯坦犊牛尿中嘌呤衍生物排出量的影响[J].中国畜牧杂志,2005,41(6):15-18.

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