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A Comparison Study on Bacteria Diversity of Rumen, Reticulum, Omasum and Abomasum from Sheep Using PCR-DGGE Analysis

  • ZENG Yan ,
  • ZENG Dong ,
  • NI Xueqin ,
  • ZHANG Yan ,
  • ZHU Hui ,
  • TANG Yurui
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  • Institute of Animal Microecology, College of Veterinary Medicine, Sichuan Agricultural University, Key Laboratory of Animal Disease and Human Health of Sichuan Province, Ya'an 625014, China

Received date: 2014-07-08

  Online published: 2015-01-10

Abstract

In order to compare the bacterial flora diversity of rumen, reticulum, omasum and abomasum from sheep, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis compounded with cloning and sequencing of bands in DGGE profiles were used, and cluster analysis and principal constituent analysis (PCA) of bacterial flora were carried out. The results showed as follows: the average number of DGGE bands of contents in rumen, reticulum, omasum and abomasum was 18, 13, 16 and 15, respectively; Shannon diversity index, evenness and richness of bacteria in rumen and omasum were higher, which were 2.83, 0.79 and 17.00, and 2.82, 0.79 and 16.80, respectively, however, those of reticulum and abomasum were lower, which were 2.52, 0.70 and 12.60, and 2.73, 0.76 and 15.40, respectively; clustering analysis results suggested that there were some differences in bacterial flora among the contents in different stomachs, but the similarity coefficients from the same segment of stomach of different animals were all above 0.63; most of the sequenced bands belonged to Bacteroidetes and Firmicutes, and Bacteroidetes, Firmicutes, uncultured rumen bacterium, uncultured bacterium and Veillonellaceae were predominant in stomach, while the special bacteria were Acinetobacter sp. and Ruminococcaceae bacteria. In conclusion, there are rich kinds and large amount of bacteria in the four stomachs of sheep. With rearward moving of position of intestinal tract, the diversity increased firstly and then decreased and finally increased.

Cite this article

ZENG Yan , ZENG Dong , NI Xueqin , ZHANG Yan , ZHU Hui , TANG Yurui . A Comparison Study on Bacteria Diversity of Rumen, Reticulum, Omasum and Abomasum from Sheep Using PCR-DGGE Analysis[J]. Chinese Journal of Animal Nutrition, 2015 , 27(1) : 298 -304 . DOI: 10.3969/j.issn.1006-267x.2015.01.036

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