Short Communications

Associative Effects of Different Combination Ratios of Silkworm Excrement and Rice Straw on the Rumen Microbial Fermentation in Vitro

  • SUN Lisa ,
  • LI Huawei ,
  • CUI Huihui ,
  • ZHAO Rui ,
  • WANG Mengzhi ,
  • YU Lihuai ,
  • WANG Hongrong
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  • College of Animal Science and Technology, University of Yangzhou, Yangzhou 225009, China

Received date: 2014-07-21

  Online published: 2015-01-10

Abstract

In vitro fermentation was conducted to investigate associative effects of different combination ratios of silkworm excrement (SE) and rice straw (RS). SE and RS were mixed at the ratios of 100: 0 (SE100 group), 80: 20 (SE80 group), 60: 40 (SE60 group), 40: 60 (SE40 group), 20: 80 (SE20 group) and 0: 100 (SE0 group), respectively. The mixtures were incubated in vitro for 24 hours for a batched in vitro fermentation test and 72 hours for an in vitro fermentation gas production test. Gas production parameters and fermentation characteristics were determined. The results showed as follows: 1) theological maximum gas production in SE100 group was significantly higher than that of the other groups (P<0.05), and accumulated gas production of SE80 group was significantly higher than that of the other groups (P<0.05). 2) Total volatile fatty acid concentration in culture medium at 24 hours showed SE80 group > SE100 group > SE60 group > SE40 group > SE0 group > SE20 group, and acetate/propionate showed SE80 group < SE40 group < SE20 group < SE60 group < SE0 group < SE100 group. 3) Microbial protein concentration in culture medium at 24 hours showed SE20 group > SE100 group > SE60 group > SE80 group > SE40 group > SE0 group. 4) With the increasing of SE ratio, in vitro digestion rate of organic matter of substrate was increased. 5) Multiply factor associative effect index of SE80 group was 0.76, and showed SE80 group > SE40 group > SE60 group > SE20 group. In conclusion, the combination of SE and RS can improve in vitro fermentation characteristics and gas production parameters, and the optimal ratio of SE and RS is 80: 20.

Cite this article

SUN Lisa , LI Huawei , CUI Huihui , ZHAO Rui , WANG Mengzhi , YU Lihuai , WANG Hongrong . Associative Effects of Different Combination Ratios of Silkworm Excrement and Rice Straw on the Rumen Microbial Fermentation in Vitro[J]. Chinese Journal of Animal Nutrition, 2015 , 27(1) : 313 -319 . DOI: 10.3969/j.issn.1006-267x.2015.01.038

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