Threonine Requirement of Juvenile Chinese Mitten Crab (Eriocheir sinensis)

WANG Wei, YE Jinyun, YANG Xia, ZHANG Yixiang, LIU Pei

doi:10.3969/j.issn.1006-267x.2015.02.018

Chinese Journal of Animal Nutrition >

2015 , Vol. 27 >Issue 2: 476 - 484

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.02.018

Aquaculture Nutrition

Threonine Requirement of Juvenile Chinese Mitten Crab (Eriocheir sinensis)

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1. School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325035, China;
2. Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding & Nutrition of Chinese Academy of Fishery Science, Huzhou University, Huzhou 313000, China;
3. Dalian Ocean University, Dalian 116023, China

Received date: 2014-08-08

Online published: 2015-02-09

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Abstract

A 56-day growth trial was conducted to determine the threonine requirement of juvenile Chinese mitten crab (Eriocheir sinensis). Six isonitrogenous and isoenergetic purified diets were formulated using casein, gelatin and crystalline amino acids as protein sources, and the threonine content in those diets was 0.86%(control, without extra threonine), 1.24%, 1.61%, 2.04%, 2.42% and 2.81% of dietary dry matter by adding crystalline L-threonine, respectively. A total of 360 juvenile Chinese mitten crab with the initial body weight of (0.36±0.00) g were chosen and randomly distributed into 6 groups with 3 replicates per group and 20 crabs per replicate. The results showed that with the increase of dietary threonine content, the weight gain rate (WGR), specific growth rate (SGR), and the activities of digestive enzymes (pepsin, trypsin, lipase and amylase) were firstly increased and then decreased, which reached their peaks in the 1.61% group, and the WGR and SGR in the 1.61% group were significantly higher than those in the 0.86%, 1.24%, 2.42% and 2.81% groups (P<0.05) and the activities of digestive enzymes in the 1.61% group were significantly higher than those in the other groups (P<0.05). Whereas, the feed conversion ratio (FCR) reached the lowest value in the 1.61% group, and the protein efficiency ratio (PER) reached the highest value in the same group. Dietary threonine content had no significant effects on survival rate (SR) and body composition (P>0.05). The activities of superoxide dismutase (SOD), alkaline phosphatase (AKP) and acid phosphatase (ACP) in hepatopancreas were firstly increased and then decreased, which reached their peaks in the 1.61%, 1.61% and 2.42% groups, respectively. Using WGR as the evaluation index, dietary threonine requirement of juvenile Chinese mitten crab is estimated to be 1.59% of dietary dry matter (3.98% of dietary protein) by broken-line regress model analysis.

Key words： juvenile Chinese mitten crab; threonine; requirement; digestive enzymes; non-specific immune indices

Cite this article

WANG Wei, YE Jinyun, YANG Xia, ZHANG Yixiang, LIU Pei . Threonine Requirement of Juvenile Chinese Mitten Crab (Eriocheir sinensis)[J]. Chinese Journal of Animal Nutrition, 2015 , 27(2) : 476 -484 . DOI: 10.3969/j.issn.1006-267x.2015.02.018

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