Effects of Exogenous Spermine on Small Intestine Mucous Fibroblast Growth Factor-2 Protein Expression and Small Intestinal Development of Weaner Piglets

HE Yuyong, ZHAO Hongtao, LU Wei

doi:10.3969/j.issn.1006-267x.2015.02.021

2015 , Vol. 27 >Issue 2: 503 - 509

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.02.021

Feed Science and Technology

Effects of Exogenous Spermine on Small Intestine Mucous Fibroblast Growth Factor-2 Protein Expression and Small Intestinal Development of Weaner Piglets

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Jiangxi Province Key Lab of Animal Nutrition, Jiangxi Agricultural University, Nanchang 330045, China

Received date: 2014-09-09

Online published: 2015-02-09

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Abstract

This experiment was conducted to investigate the effects of exogenous spermine on small intestine mucous fibroblast growth factor-2 (FGF2) protein expression and small intestinal development of weaner piglets. Nine litters of "Duroc×Landrace×Large White" crossbred piglets were randomly divided into three groups with 3 litters in each group, and piglets in the same group received a diet supplemented with exogenous spermine at a level of 0, 12 and 15 mg/kg from 7 to 28 days of age. Two piglets with the average body weight from each litter were slaughtered and small intestinal samples were collected for the determination of FGF2 protein expression and small intestinal development. The results showed as follows: 1) the small intestine mucous FGF2 protein expression of piglets in 12 mg/kg exogenous spermine groups was higher than that in 0 and 15 mg/kg exogenous spermine groups, respectively. However, there was no significant difference on small intestine mucous FGF2 protein expression of piglets among 3 exogenous spermine groups (P>0.05). 2) The villous height and width of piglets in 12 and 15 mg/kg exogenous spermine groups were significantly higher than that in 0 mg/kg exogenous spermine group (P<0.01), the villus height/crypt depth (V/C) in duodenum and jejunum of piglets in 12 mg/kg exogenous spermine group were significantly higher than that in 0 mg/kg exogenous spermine group (P<0.01), respectively. 3) The activities of sucrase and maltase in small intestine mucous of piglets at 28 days of age increased with the addition of exogenous spermine increased. In conclusion, dietary continuity supplemented with exogenous spermine of piglets at 7 to 28 days of age can increase small intestine mucous FGF2 protein expression, V/C and disaccharidase activity of piglets at 28 days of age.

Key words： exogenous spermine; small intestine; FGF2 protein expression; morphology; disaccharidase; piglets

Cite this article

HE Yuyong, ZHAO Hongtao, LU Wei . Effects of Exogenous Spermine on Small Intestine Mucous Fibroblast Growth Factor-2 Protein Expression and Small Intestinal Development of Weaner Piglets[J]. Chinese Journal of Animal Nutrition, 2015 , 27(2) : 503 -509 . DOI: 10.3969/j.issn.1006-267x.2015.02.021

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