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Effects of Tannin and Polyethylene Glycol on Ruminal Cellulolytic Bacteria Quantity in Sheep and Goats

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  • College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2014-08-04

  Online published: 2015-02-09

Abstract

This paper studied the effects of different dietary tannin levels and high tannin diets supplemented with polyethylene glycol (PEG) on ruminal cellulolytic bacteria quantity in sheep and goats. Four sheep and four cashmere goats aged 1.5 years, weighted 45 kg and fitted with permanent ruminal cannulas were selected as experimental animals. Own control experimental design was used, and the study was conducted in consecutive four periods. Animals in the four periods were fed a basal diet (period 1, group Ⅰ), and the basal diet supplemented with 2% tannin (period 2, group Ⅱ), 6% tannin (period 3, group Ⅲ) and 6% tannin + 12% PEG (period 4, group Ⅳ), respectively. Each period lasted for 30 days consisted of a 12-day pre-test period and 18-day test period. On the 1st day of each test period, ruminal content was collected, rumen fluid pH, and the concentrations of ammonia nitrogen (NH3-N) and microbial protein (MCP) were measured, and real-time PCR was used to quantify R. albus, R. flavefaciens and F. succinogenes adherent to solid phase, liquid phase and full chyme. The results showed that as follows: 1) compared with group Ⅰ, the supplementation of 6% tannin significantly decreased NH3-N concentration in rumen fluid in goats and MCP concentration in rumen fluid of sheep (P<0.05). These two indices recovered to the same level with group Ⅰ after the supplementation of 6% tannin+12% PEG. 2) Compared with group Ⅰ, the supplementation of 6% tannin significantly decreased the quantity of R. albus in solid phase of ruminal content in sheep and goats, and in liquid phase of ruminal content in sheep (P<0.05); the supplementation of 2% and 6% tannin significantly decreased the quantity of R. flavefaciens in solid phase of ruminal content in sheep and goats (P<0.05), and the supplementation of 6% tannin significantly decreased the quantity of R. flavefaciens in liquid phase of ruminal content in sheep and goats (P<0.05); the supplementation of 6% tannin significantly decreased the quantity of F. succinogenes in solid phase of ruminal content of sheep and goats, and in full chyme of ruminal content in sheep (P<0.05). The quantity of the three cellulolytic bacteria recovered to the same or above level with group Ⅰ after the supplementation of 6% tannin+12% PEG. In conclusion, dietary supplementation of 6% tannin can decrease NH3-N concentrations, affect MCP synthesis in rumen fluid, and reduce the quantity of cellulolytic bacteria in rumen; the negative effects of tannin on proliferation of ruminal cellulolytic bacteria were impaired by adding PEG, ruminal fermentation were also improved.

Cite this article

LI Dabiao, ZHANG Meimei, YU Yongqiang, LI Honglei, TA Na, XING Yuanyuan, WANG Weiyun . Effects of Tannin and Polyethylene Glycol on Ruminal Cellulolytic Bacteria Quantity in Sheep and Goats[J]. Chinese Journal of Animal Nutrition, 2015 , 27(2) : 596 -605 . DOI: 10.3969/j.issn.1006-267x.2015.02.032

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