Effects of Umbilical Cord Mesenchymal Stem Cells Transplantation on Body Weight, Serum Immune and Antioxidant Levels of Holstein Bull Calves

ZHAO Yankun, WANG Liwen, PENG Guoliang, SHAO Wei, YU Xiong

doi:10.3969/j.issn.1006-267x.2015.02.034

2015 , Vol. 27 >Issue 2: 616 - 622

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.02.034

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Effects of Umbilical Cord Mesenchymal Stem Cells Transplantation on Body Weight, Serum Immune and Antioxidant Levels of Holstein Bull Calves

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Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China

Received date: 2014-08-04

Online published: 2015-02-09

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Abstract

This article aimed to explore the effects of umbilical cord mesenchymal stem cells (UC-MSCs) transplantation on body weight (BW), serum immune and antioxidant levels of Holstein bull calves. Twenty eight 1-month-old Holstein bull calves with similar body weight were selected and randomly divided into control and experimental groups with 14 calves per group. Fresh fetal umbilical cord tissue was collected from healthy new born Holstein calves. UC-MSCs were purified and cultivated in vitro by the method of pancreatic enzyme digestion. Calves of experimental group were injected in jugular vein with 3×10⁵ cells/kg BW UC-MSCs (diluted to 8 mL), while those of control group were injected with normal saline at equal dose. The injection was carried out at 1, 2, 3, 4 and 5 months of age, respectively. The test lasted for 150 d. The results showed as follows: compared with control group, the transplantation of UC-MSCs in bull calves, 1) significantly increased BW at 4, 5 and 6 months of age (P<0.05); significantly increased average daily gain at 3 (P<0.05), 4 (P<0.05) and 6 months of age (P<0.01); 2) significantly increased serum immunoglobulin A (IgA) content at 4 (P<0.01) and 6 months of age (P<0.05); significantly increased serum immunoglobulin G (IgG) at 2 (P<0.01), 5 (P<0.01) and 6 months of age (P<0.05); significantly decreased serum interleukin-2 (IL-2) content at 3 (P<0.01) and 4 months of age (P<0.05); 3) significantly decreased serum malondialdehyde (MDA) content at 2, 3, 4, 5 and 6 months of age (P<0.01); significantly increased serum superoxide dismutase (SOD) activity at 5 (P<0.05) and 6 months of age (P<0.01). Thus, the transplantation of UC-MSCs can increase serum IgA and IgG contents, decrease serum MDA content, increase serum SOD activity, enhance the body's ability to clear free radicals, reduced serum IL-2 content, and reduced internal inflammation, thereby enhance immunity, and stimulate the growth and development of bull calves.

Key words： umbilical cord mesenchymal stem cells; Holstein bull calves; immunity; antioxidant; average daily gain

Cite this article

ZHAO Yankun, WANG Liwen, PENG Guoliang, SHAO Wei, YU Xiong . Effects of Umbilical Cord Mesenchymal Stem Cells Transplantation on Body Weight, Serum Immune and Antioxidant Levels of Holstein Bull Calves[J]. Chinese Journal of Animal Nutrition, 2015 , 27(2) : 616 -622 . DOI: 10.3969/j.issn.1006-267x.2015.02.034

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