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Effect of Codonopsis pilosula as an Immunopotentiator on Intestinal Bacterial Community Composition of Apostichopus japonicus

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  • Marine Biology Institute of Shandong Province, Key Laboratory for Disease Control in Mariculture of Shandong Province, Qingdao 266002, China

Received date: 2014-09-16

  Online published: 2015-02-09

Abstract

By plate counting method and polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) technology, the effect of Codonopsis pilosula as an immunopotentiator on intestinal bacterial community composition of Apostichopus japonicas was investigated. Apostichopus japonicas with the initial body weight of (18.00±2.00) g were randomly divided into 2 groups (control group and experimental group) with 6 replicates per group and 12 Apostichopus japonicas per replicate. Apostichopus japonicas in the control group were fed a diet which were formulated using sea mud and Sargassum thunbergii powder with the mass ratio of sea mud to Sargassum thunbergii ratio was 1:1, while those in the experimental group were fed the diet adding Codonopsis pilosula with the quantity of 2% Sargassum thunbergii powder weight. The feeding trial lasted for 28 days. The results showed that immunopotentiator-Codonopsis pilosula could significantly promote the special growth rate (P<0.05), reduce the feed conversion ratio (P<0.05), and increase the count of heterotrophic in intestinal content of Apostichopus japonicas (P<0.05). Sequence statistics showed that the proportion of superior sequence was significantly increased after feeding Codonopsis pilosula (P<0.05), which in experimental group was 97.57% and that in control group was only 80.22%. Beta-diversity analysis reported that the intestinal microenvironment of Apostichopus japonicas was changed after feeding Codonopsis pilosula, and the rages of coefficient variation were 14.91% to 15.47%, 15.47% to 16.21%, and 14.91% to 16.21%. Abundance analysis showed that the abundances of Proteobacteria and Bacteroidetes were increased, but the abundances of Verrucomicrobia, Actinobacteria and Firmiaites were decreased after feeding Codonopsis pilosula. The similarity coefficient of intestinal bacterial community composition between control group and experimental group was 0.97 by cluster analysis. It is concluded that Codonopsis pilosula as an immunopotentiator can improve the specific growth rate, reduce the feed conversion ratio, increase the count of heterotroph and the abundance of dominant bacteria in intestine, and optimize the intestinal microenvironment of Apostichopus japonicus.

Cite this article

FAN Ying, LI Le, YU Xiaoqing, LIU Enfu, LI Tianbao, XU La, YE Haibin . Effect of Codonopsis pilosula as an Immunopotentiator on Intestinal Bacterial Community Composition of Apostichopus japonicus[J]. Chinese Journal of Animal Nutrition, 2015 , 27(2) : 638 -646 . DOI: 10.3969/j.issn.1006-267x.2015.02.037

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