Effects of Probiotics on Growth Performance, Faecal Microflora Number, Nutrient Apparent Digestibility and Serum Immune Indices of Growing Pigs

LIU Hui, JI Haifeng, WANG Sixin, ZHANG Dongyan, WANG Jing, SHAN Dacong, WANG Yamin

doi:10.3969/j.issn.1006-267x.2015.03.021

2015 , Vol. 27 >Issue 3: 829 - 837

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.03.021

Feed Science and Technology

Effects of Probiotics on Growth Performance, Faecal Microflora Number, Nutrient Apparent Digestibility and Serum Immune Indices of Growing Pigs

Expand

Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

Received date: 2014-09-22

Online published: 2015-03-18

Fold

Abstract

This experiment was conducted to study the effects of probiotics (Lactobacillus brevis, feed yeast and their complex probiotics) on growth performance, faecal microflora number, nutrient apparent digestibility and serum immune indices of growing pigs. A total of 108 "Duroc×Landrace×Yorkshire" crossbred growing pigs with an average body weight of (13.68±0.41) kg were randomly distributed into 4 groups with 3 replicates per group and 9 pigs per replicate comprising of control group (CT), Lactobacillus brevis group (LB), yeast group (YT) and complex probiotics group (LBY), respectively. The total test period lasted for 61 days in 2 phases (phase Ⅰ, day 1 to 32; phase Ⅱ, day 33 to 61). The results showed as follows: 1) in phase Ⅰ, compared with CT, the final weight (FW) in probiotics groups and the average daily gain (ADG) in LB and LBY were significantly increased (P<0.05), while feed/gain in LB and YT was significantly decreased (P<0.05); in phase Ⅱ, the FW in LBY was significantly increased compared with CT (P<0.05); in total test period, compared with CT, the ADG in LB and LBY was significantly increased (P<0.05), while the feed/gain in LBY was significantly decreased (P<0.05). 2) On day 32, compared with CT, the number of faecal yeast in probiotics groups was significantly increased (P<0.05), and there was a significantly increase in the number of faecal Lactobacillus (P<0.05) and a significantly decrease in the number of faecal E.coli in LB and LBY (P<0.05); on day 61, compared with CT, the number of faecal Lactobacillus and yeast in probiotics groups was significantly increased (P<0.05), and the number of faecal E.coli in LB and LBY was significantly decreased (P<0.05). 3) On day 32, the apparent digestibility of crude protein (CP) and calcium in LB and LBY, and the apparent digestibility of calcium and phosphorus in YT were significantly increased compared with CT (P<0.05); on day 61, the apparent digestibility of CP, calcium and phosphorus in LB and LBY, and the apparent digestibility of calcium and phosphorus in YT were significantly increased compared with CT (P<0.05). 4) On day 32, compared with CT, serum immunoglobulin (Ig) A content in probiotics groups and serum total protein (TP), globulin (GLB) and IgG contents in LBY were significantly increased (P<0.05); on day 61, compared with CT, serum TP, GLB, IgA and IgG contents in LB, serum TP and IgG contents in YT, and serum GLB, IgA and IgG contents in LBY were significantly increased (P<0.05). It is concluded that probiotics supplementation can improve the intestinal microbial environment, elevate the apparent digestibility of diet nutrients, and strengthen the immune function, and then enhance the growth performance of growing pigs.

Key words： growing pigs; probiotics; growth performance; faecal microflora; apparent digestibility; immune indices

Cite this article

LIU Hui, JI Haifeng, WANG Sixin, ZHANG Dongyan, WANG Jing, SHAN Dacong, WANG Yamin . Effects of Probiotics on Growth Performance, Faecal Microflora Number, Nutrient Apparent Digestibility and Serum Immune Indices of Growing Pigs[J]. Chinese Journal of Animal Nutrition, 2015 , 27(3) : 829 -837 . DOI: 10.3969/j.issn.1006-267x.2015.03.021

References

[1] GIANG H H,VIET T Q,OGLE B,et al.Growth performance,digestibility,gut environment and health status in weaned piglets fed a diet supplemented with potentially probiotic complexes of lactic acid bacteria[J].Livestock Science,2010,129(1/2/3):95-103.

[2] PIEPER R,JANCZYK P,URUBSCHUROV V,et al.Effect of Lactobacillus plantarum on intestinal microbial community composition and response to enterotoxigenic Escherichia coli challenge in weaning piglets[J].Livestock Science,2010,133(1/2/3):98-100.

[3] 文静,孙建安,周绪霞,等.屎肠球菌对仔猪生长性能、免疫和抗氧化功能的影响[J].浙江农业学报,2011,23(1):70-73.

[4] TORRALLARDONA D,CONDE M R,BADIOLA T,et al.Effect of fishmeal replacement with spray-dried animal plasma and colistin on intestinal structure,intestinal microbiology,and performance of weanling pigs challenged with Escherichia coli K99[J].Journal of Animal Science,2003,81(5):1220-1226.

[5] 朱燕,夏玉宇.饲料品质检验[M].北京:化学工业出版社,2003.

[6] BROOM L J,MILLER H M,KERR K G,et al.Effects of zinc oxide and Enterococcus faecium SF68 dietary supplementation on the performance, intestinal microbiota and immune status of weaned piglets[J].Research in Veterinary Science,2006,80(1):45-54.

[7] HARDING S V,FRASER K G,WYKES L J.Probiotics stimulate liver and plasma protein synthesis in piglets with dextran sulfate-induced colitis and macronutrient restriction[J].The Journal of Nutrition,2008,138(11):2129-2135.

[8] DAVIS M E,BROWN D C,BAKER A,et al.Effect of direct-fed microbial and antibiotic supplementation on gastrointestinal microflora,mucin histochemical characterization,and immune populations of weanling pigs[J].Livestock Science,2007,108(1/2/3):249-253.

[9] VAN HEUGTEN E,FUNDERBURKE D W,DORTON K L.Growth performance,nutrient digestibility,and fecal microflora in weanling pigs fed live yeast[J].Journal of Animal Science,2003,81(4):1004-1012.

[10] 陈文辉,周映华,李秋云,等.复合益生菌制剂对断奶仔猪生产性能和腹泻率的影响[J].湖南畜牧兽医,2007(3):14-15.

[11] BOMBA A,NEMCOVÁ R,MUDROÑOVÁ D,et al.The possibilities of potentiating the efficacy of probiotics[J].Trends in Food Science & Technology,2002,13(4):121-126.

[12] 辛娜,张乃锋,刁其玉,等.芽孢杆菌制剂对断奶仔猪生长性能、胃肠道发育的影响[J].畜牧兽医学报,2012,43(6):901-908.

[13] CHESSON A.Probiotics and other intestinal mediators[M]//COLE D J A,WISIMAN J,VARLEY M A.Principles of pig science.Loughbourough:Nottingham University Press,1994:197-214.

[14] 毛倩,陈代文,余冰,等.复合益生素对生长育肥猪生产性能、盲肠菌群及代谢产物的影响[J].中国畜牧杂志,2010,46(17):34-38.

[15] 周盟,张乃锋,董晓丽,等.益生菌对断奶仔猪生长性能、免疫器官指数及胃肠道pH的影响[J].动物营养学报,2014,26(2):445-452.

[16] GÖRKE B,LIEBLER-TENORIO E.Probiotics:is there a scientific basis for their effects?[J].Dtsch Tierärztliche Wochenschrift,2001,108(6):249-251.

[17] PLUSKE J R,HAMPSON D J,WILLIAMS I H.Factors influencing the structure and function of the small intestine in the weaned pig:a review[J].Livestock Production Science,1997,51(1/2/3):215-236.

[18] SALMINEN S,VON WRIGHT A,OUWEHAND A.Lactic acid bacteria microbiological and functional aspects[M].New York:Marcel Dekker Inc,2004:547-580.

[19] SANDERS M E,HUIS IN'T VELD J.Bringing a probiotic-containing functional food to the market:microbiological,product,regulatory and labeling issues[J].Antonie van Leeuwenhoek,1999,76(1/2/3/4):293-315.

[20] HUANG C H,QIAO S Y,LI D F,et al.Effects of lactobacilli on the performance,diarrhea incidence,VFA concentration and gastrointestinal microbial flora of weaning pigs[J].Asian-Australasian Journal of Animal Science,2004,17(3):401-409.

[21] CLEVELAND J,MONTVILLE T J,NES I F,et al.Bacteriocins:safe,natural antimicrobials for food preservation[J].International Journal of Food Microbiology,2001,71(1):1-20.

[22] NAUGHTON P J,MIKKELSEN L L,JENSEN B B.Effects of nondigestible oligosaccharides on Salmonella enterica serovar Typhimurium and nonpathogenic Escherichia coli in the pig small intestine in vitro[J].Applied and Environmental Microbiology,2001,67(8):3391-3395.

[23] WHITE L A,NEWMAN M C,CROMWELL G L,et al.Brewers dried yeast as a source of mannan oligosaccharides for weanling pigs[J].Journal of Animal Science,2002,80(10):2619-2628.

[24] 胡友军,林映才,余德谦,等.活性酵母对早期断奶仔猪肠道微生物区系、肠黏膜形态和挥发性盐基氮的影响[J].养猪,2003(4):3-5.

[25] 张玲清.影响猪消化率的因素分析[J].中国畜牧兽医,2009,36(9):174-178.

[26] 王志祥,乔家运,王自恒,等.乳酸杆菌对断奶仔猪生长性能、养分表观消化率和消化酶活性的影响[J].西北农林科技大学学报:自然科学版,2006,34(4):23-27.

[27] 洪奇华,陈安国,黄建平.富酶活性酵母对仔猪生产性能的影响[J].养猪,2005(2):7-8.

[28] KUNAVUE N,LIEN T F.Effects of fulvic acid and probiotic on growth performance,nutrient digestibility,blood parameters and immunity of pigs[J].Journal of Animal Science Advances,2012,2(8):711-721.

[29] 曹廷富.微生态制剂对生长猪生产指标和粪中微生物影响的研究[D].硕士学位论文.长沙:湖南农业大学,2011:15-18.

[30] HÖGBERG A,LINDBERG J E.The effect of level and type of cereal non-starch polysaccharides on the performance,nutrient utilization and gut environment of pigs around weaning[J].Animal Feed Science and Technology,2006,127(3/4):200-219.

[31] HENTGES D J.Gut flora and disease resistance[M]//HENTGES D J.Probiotics:the scientific basis.Netherlands:Springer,1992:87-110.

[32] 胡东兴,潘康成.微生态制剂及其作用机理[J].中国饲料,2001,1(3):14-16.

[33] JIN L Z,HO Y W,ABDULLAH N,et al.Influence of dried Bacillus substillis and Lactobacilli cultures on intestinal microflora and performance in broilers[J].Asian-Australasian Journal of Animal Sciences,1996,9(4):397-404.

[34] 李路胜.酵母培养物在家禽上的应用[J].饲料工业,2008,29(22):17-19.

[35] 韩爱云,张国强,黄仁录,等.二氢杨梅素对肉仔鸡血液生化指标及生长性能的影响[J].中国兽医杂志,2007,43(11):19-21.

[36] 董晓丽,张乃锋,周盟,等.复合菌制剂对断奶仔猪生长性能、粪便微生物和血清指标的影响[J].动物营养学报,2013,25(6):1285-1292.

[37] 刘辉,季海峰,张董燕,等.饲粮添加短乳杆菌对生长猪生长性能和血清生化指标的影响[J].动物营养学报,2013,25(1):182-189.

[38] 何明清,程安春.动物微生态学[M].2版.成都:四川科学技术出版社,2004:201-208.

[39] INDEGRREW W M.Yeast:could you have a business on this bug[C]//Biotechnology in the feed industry.Loughborough,Leics.Nottingham University Press, 1999,15:27-50.

[40] SHEN Y B,PIAO X S,KIM S W,et al.Effects of yeast culture supplementation on growth performance,intestinal health,and immune response of nursery pigs[J].Journal of Animal Science,2009,87(8):2614-2624.

[41] 李瑞,侯改凤,邬理洋,等.微生态制剂对生长猪生产性能、氮磷排放量及血清免疫指标的影响[J].家畜生态学报,2013,34(6):66-71.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References