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Effects of Different Levels of Metabolic Glucose on Artificial Rumen Fermentation Characteristics, Microbial Protein Concentration and Gas Production Parameters of Sheep

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  • College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2014-09-09

  Online published: 2015-03-18

Abstract

This experiment aimed to investigate rumen fermentation characteristics, microbial protein concentration and gas production parameters of sheep under the different levels of metabolic glucose through the in vitro artificial rumen fermentation. A single factor experiment design with 4 metabolic glucose levels [125 (A), 138 (B), 153 (C) and 168 g/kg(D)] was adopted. Culture medium of 2 mL was collected for analysis at 0, 2, 4, 6, 8, 12 and 24 hours, respectively. The results showed as follows: 1) with the increasing of metabolic glucose level, the pH of culture medium was significantly decreased at 8 to 24 h (P<0.05 or P<0.01); at 2 h, the ammonia nitrogen concentration of group D was significantly higher than that of group A (P<0.05), while at 6 h, the concentration of group A was significantly higher than that of the other three groups (P<0.05). 2) The bacterial protein concentration at 6 h of group D was significantly higher than that of group A (P<0.05); with the increasing of metabolic glucose level, the concentrations of propionic acid, butyric acid and total volatile fatty acids were increased in general, while acetate/propionate was decreased. 3) With the increasing of metabolic glucose level, the theological maximum gas production and reaching time of 1/2 of the theological maximum gas production were significantly reduced (P<0.01); the potential gas production has no significant change (P>0.05); the 24 h gas production and the constant of gas production rate were significantly decreased and increased (P<0.05 or P<0.01), respectively. The results indicate that the increasing of metabolic glucose level can improve the concentrations of propionic acid and total volatile fatty acids, meanwhile, can provide more sugar precursors for the sheep.

Cite this article

ZHAO Rui, XU Jinhao, LI Huawei, WANG Hongrong . Effects of Different Levels of Metabolic Glucose on Artificial Rumen Fermentation Characteristics, Microbial Protein Concentration and Gas Production Parameters of Sheep[J]. Chinese Journal of Animal Nutrition, 2015 , 27(3) : 932 -940 . DOI: 10.3969/j.issn.1006-267x.2015.03.034

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