Effects of Different Carbon/Nitrogen Ratios in Cultured Water under Zero-Water Exchange Condition on Bioflocs Formation and Intestinal Microflora Structure of Blunt Anout Bream (Megalobrama amblycephala)

SUN Shengming, ZHU Jian, GE Xianping, JIANG Xiaojun

doi:10.3969/j.issn.1006-267x.2015.03.036

2015 , Vol. 27 >Issue 3: 948 - 955

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.03.036

Short Communications

Effects of Different Carbon/Nitrogen Ratios in Cultured Water under Zero-Water Exchange Condition on Bioflocs Formation and Intestinal Microflora Structure of Blunt Anout Bream (Megalobrama amblycephala)

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1. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;
2. WuXi Fishery College, Nanjing Agricultural University, Wuxi 214081, China

Received date: 2014-09-22

Online published: 2015-03-18

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Abstract

This present study was conducted to investigate the effects of different carbon (C)/nitrogen (N) ratios in cultured water under zero-water exchange condition on bioflocs formation and intestinal microflora structure of blunt anout bream (Megalobrama amblycephala) using by 454 pyrosequencing technology and PCR-denaturing gradient gel electrophoresis (DGGE) technology. Five groups were designed, and the C/N ratios in those groups were 8(CN8 group, as control group), 12(CN12 group), 16(CN16 group), 20(CN20 group) and 24(CN24 group), respectively. Each group had 3 tanks (replicates), and each tank cultured 20 juvenile blunt anout bream. Culture period was 8 weeks, and water samples were collected every other week to detect the contents of ammonia nitrogen, nitrite nitrogen and bioflocs in cultured water. After 8 weeks culture, three fish were selected in each tank, and the cecal contents of those fish were collected to analyze the change of intestinal microflora structure of blunt anout bream cultured in different C/N ratios under zero-water exchange tanks using by 454 pyrosequencing technology. The results showed that when the C/N ratio was over 16, the biofloc could effectively regulate water quality, and reduce ammonia nitrogen and nitrite nitrogen contents in water. With the C/N ratio increased from 8 to 16 and 20, intestinal Actinobateria content had no significant change (P>0.05), and Firmicutes content was significantly increased (P<0.05), while the contents of Fusobateria and Proteobacteria were significantly decreased (P<0.05). For Firmicutes, a further analysis was made, at class level, the content of intestinal Clostridia in CN16, CN20 and CN24 groups was significantly decresed compared with control group (P<0.05), while the content of intestinal Bacilli significantly increased (P<0.05). The DGGE fingerprints also demonstrated that the intestinal microflora community diversity of blunt anout bream was affected by C/N ratio in cultured water. The similarity coefficient between CN8 group and other groups was 0.43, which was the lowest. CN20 and CN24 groups were related to each other with the similarity coefficient was 0.96, which was the higest. It is concluded that the formation of bioflocs is affected and the diversity and structure of intestinal microflora of blunt anout bream are altered by C/N ratio in cultured water under zero-water exchange condition. Using the intestinal microflora structure of blunt anout bream as an evaluation index, the suitable C/N ratio in cultured water under zero-water exchange condition is 16 to 20.

Key words： blunt anout bream (Megalobrama amblycephala); intestinal microflora; 454 pyrosequencing; C/N ratio

Cite this article

SUN Shengming, ZHU Jian, GE Xianping, JIANG Xiaojun . Effects of Different Carbon/Nitrogen Ratios in Cultured Water under Zero-Water Exchange Condition on Bioflocs Formation and Intestinal Microflora Structure of Blunt Anout Bream (Megalobrama amblycephala)[J]. Chinese Journal of Animal Nutrition, 2015 , 27(3) : 948 -955 . DOI: 10.3969/j.issn.1006-267x.2015.03.036

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