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Cloning and Sequence Analysis of Full-Length cDNAs of Peroxisome Proliferator-Activated Receptor α, Lipoprotein Lipase and Hepatic Lipase in Liver of Siberin Sturgen (Acipenser baerii)

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  • 1. National Aquafeed Safety Assessment Station, Feed Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China;
    2. Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Beijing 100081, China

Received date: 2014-09-15

  Online published: 2015-03-18

Abstract

The full-length cDNAs of peroxisome proliferator-activated receptor α (PPARα), lipoprotein lipase (LPL) and hepatic lipase (HL) were cloned from Siberian sturgeon (Acipenser baerii) liver using reverse transcription-phosphatase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE) methods in this study. The results showed as follows: the full-length cDNA of PPARα gene in liver of Siberian sturgeon (GenBank accession No. KJ534588) was 1 736 bp, including a 5' untranslated region of 158 bp, an open reading frame (ORF) of 1 401 bp and a 3' untranslated region of 177 bp, the ORF encoding a protein of 466 amino acids with the theoretical molecular weight of 52.3 ku, and shared 75.5% to 82.4% similarity with other species. The full-length cDNA of LPL gene in liver of Siberian sturgeon (GenBank accession No. KJ720972) was 1 760 bp, including a 5' untranslated region of 163 bp, an ORF of 1 506 bp and a 3' untranslated region of 91 bp, the ORF encoding a protein of 501 amino acids with the theoretical molecular weight of 57.1 ku, and shared 48.7% to 67.0% similarity with other species. The full-length cDNA of HL gene in liver of Siberian sturgeon (GenBank accession No. KJ720973) was 1 740 bp, including a 5' untranslated region of 124 bp, an ORF of 1 500 bp and a 3' untranslated region of 116 bp, the ORF encoding a protein of 499 amino acids with the theoretical molecular weight of 56.4 ku, and shared 55.4% to 67.1% similarity with other species. Phylogenetic tree analysis revealed that PPARα gene of Siberian sturgeon had closer genetic relationship with higher vertebrates, but LPL and HL genes had closer genetic relationship with other fishes. In this study, the gene homology and phylogenetic status of PPARα, HL, LPL from Siberian sturgeon are analyzed, and the results provide a molecular biological basis for the further study of the regulatory mechanism of lipornetabolism in Siberian sturgeon.

Cite this article

AI Lichuan, YU Xiaotong, WANG Jia, XUE Min, WU Xiufeng, ZHENG Yinhua, HAN Fang . Cloning and Sequence Analysis of Full-Length cDNAs of Peroxisome Proliferator-Activated Receptor α, Lipoprotein Lipase and Hepatic Lipase in Liver of Siberin Sturgen (Acipenser baerii)[J]. Chinese Journal of Animal Nutrition, 2015 , 27(3) : 956 -970 . DOI: 10.3969/j.issn.1006-267x.2015.03.037

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