Mitigative Effect of Taurine on Lipopolysaccharides-Induced Liver Injury in Mice

FU Ling, SONG Jianbo, HU Chunyan, ZENG Liming

doi:10.3969/j.issn.1006-267x.2015.03.040

Chinese Journal of Animal Nutrition >

2015 , Vol. 27 >Issue 3: 989 - 996

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.03.040

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Mitigative Effect of Taurine on Lipopolysaccharides-Induced Liver Injury in Mice

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Jiangxi Agricultural University, Nanchang 330045, China

Received date: 2014-10-21

Online published: 2015-03-18

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Abstract

This study aimed to investigate the mitigative effect of taurine on lipopolysaccharides (LPS)-induced liver injury in mice. Thirty ICR male mice with the similar body weight of (22±3) g were randomly divided into three groups (ten mice in each group, n=10): a control group and a LPS group in which mice received a basal diet, and a taurine group in which mice received the basal diet+2.5% taurine. After fed 1 week, mice in the LPS group and taurine group were intraperitoneally injected 10 mg/kg LPS (LPS was dissolved in physiological saline, and injection dose was 0.2 mL per mouse) and mice in the control group were intraperitoneally injected the same dose of physiological saline. Blood samples were harvested from eyes and all mice were killed at 24 h after injection to collect liver. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were measured and liver weight, oxidative stress parameters, and the relative expression of antioxidant genes, nuclear factor E2-related factor 2 (Nrf2) and Kelch like ECH associated protein 1 (Keap1) were determined. The results showed as follows: 1) LPS treatment significantly increased liver index, while taurine supplementation significantly reduced the liver index to normal level (P<0.05). 2) LPS treatment significantly enhanced serum ALT and AST activities (P<0.05), and taurine supplementation exhibited some beneficial effects on serum ALT and AST activities, and no significant difference in serum AST activity between taurine group and control group (P>0.05). 3) LPS caused marked liver injury in mice, and mice in taurine group suffered little liver injury. 4) Compared with control group, liver malondialdehyde (MDA) content was significantly increased and live glutathione peroxidase (GPx) activity was significantly decreased in LPS group (P<0.05), while those parameters in taurine group had no significant changes (P>0.05). 5) Taurine significantly mitigated the LPS-induced inhibition of GPx1 and Nrf2 expression (P<0.05). In conclusion, diet supplemented with 2.5% taurine plays a certain mitigative effect on LPS-induced liver injury in mice.

Key words： taurine; liver injury; oxidative stress; LPS; mouse

Cite this article

FU Ling, SONG Jianbo, HU Chunyan, ZENG Liming . Mitigative Effect of Taurine on Lipopolysaccharides-Induced Liver Injury in Mice[J]. Chinese Journal of Animal Nutrition, 2015 , 27(3) : 989 -996 . DOI: 10.3969/j.issn.1006-267x.2015.03.040

References

[1] RIPPS H,SHEN W.Review:taurine:a "very essential" amino acid[J].Molecular Vision,2012,18:2673-2686.

[2] STIPANUK M H,UEKI I.Dealing with methionine/homocysteine sulfur:cysteine metabolism to taurine and inorganic sulfur[J].Journal of Inherited Metabolic Disease,2011,34(1):17-32.

[3] STIPANUK M H.Role of the liver in regulation of body cysteine and taurine levels:a brief review[J].Neurochemical Research,2004,29(1):105-110.

[4] WEI S D,HUANG Q Y,LI J Z,et al.Taurine attenuates liver injury by downregulating phosphorylated p38 MAPK of Kupffer cells in rats with severe acute pancreatitis[J].Inflammation,2012,35(2):690-701.

[5] REDMOND H P,WANG J H,BOUCHIER-HAYES D.Taurine attenuates nitric oxide-and reactive oxygen intermediate-dependent hepatocyte injury[J].Archives of Surgery,1996,131(12):1280-1287.

[6] MAIA A R,BATISTA T M,VICTORIO J A,et al.Taurine supplementation reduces blood pressure and prevents endothelial dysfunction and oxidative stress in post-weaning protein-restricted rats[J].PLoS One,2014,9(8):e105851.

[7] LI Q,LIU Y L,CHE Z Q,et al.Dietary L-arginine supplementation alleviates liver injury caused by Escherichia coli LPS in weaned pigs[J].Innate Immunity,2012,18(6):804-814.

[8] ROLLER J,LASCHKE M W,SCHEUER C,et al.Heme oxygenase (HO)-1 protects from lipopolysaccharide (LPS)-mediated liver injury by inhibition of hepatic leukocyte accumulation and improvement of microvascular perfusion[J].Langenbeck's Archives of Surgery,2010,395(4):387-394.

[9] GURUNG R B,PURBE B,GYAWALI P,et al.The ratio of aspartate aminotransferase to alanine aminotransferase (AST/ALT):the correlation of value with underlying severity of alcoholic liver disease[J].Kathmandu University Medical Journal,2013,11(43):233-236.

[10] TASCI I,MAS N,MAS M R,et al.Ultrastructural changes in hepatocytes after taurine treatment in CCl₄ induced liver injury[J].World Journal of Gastroenterology,2008,14(31):4897-4902.

[11] WETTSTEIN M,HÄUSSINGER D.Taurine attenuates cold ischemia-reoxygenation injury in rat liver[J].Transplantation,2000,69(11):2290-2296.

[12] DEMINICE R,ROSA F T,DA SILVA L E C M,et al.Taurine supplementation does not decrease homocysteine levels and liver injury induced by a choline-deficient diet[J].Life Sciences,2014,105(1/2):43-47.

[13] LIU Y T,YANG L Q,TAO K M,et al.Protective effects of hydrogen enriched saline on liver ischemia reperfusion injury by reducing oxidative stress and HMGB1 release[J].BMC Gastroenterology,2014,14(1):12.

[14] MATSUO K,SASAKI E,HIGUCHI S,et al.Involvement of oxidative stress and immune- and inflammation-related factors in azathioprine-induced liver injury[J].Toxicology Letters,2014,224(2):215-224.

[15] ZHU X,ZHANG F,ZHOU L,et al.Diallyl trisulfide attenuates carbon tetrachloride-caused liver injury and fibrogenesis and reduces hepatic oxidative stress in rats[J].Naunyn-Schmiedeberg's Archives of Pharmacology,2014,387(5):445-455.

[16] KIM K J,HONG H D,LEE O H,et al.The effects of Acanthopanax senticosus on global hepatic gene expression in rats subjected to heat environmental stress[J].Toxicology,2010,278(2):217-223.

[17] ZHANG Z Y, LIU D,YI B,et al.Taurine supplementation reduces oxidative stress and protects the liver in an iron-overload murine model[J].Molecular Medicine Reports,2014,10(5):2255-2262.

[18] JEON S H,LEE M Y,RAHMAN M M,et al.The antioxidant,taurine reduced lipopolysaccharide (LPS)-induced generation of ROS,and activation of MAPKs and Bax in cultured pneumocytes[J].Pulmonary Pharmacology & Therapeutics,2009,22(6):562-566.

[19] CHANG Y Y,CHOU C H,CHIU C H,et al.Preventive effects of taurine on development of hepatic steatosis induced by a high-fat/cholesterol dietary habit[J].Journal of Agricultural and Food Chemistry,2011,59(1):450-457.

[20] CHEN X,SEBASTIAN B M,TANG H,et al.Taurine supplementation prevents ethanol-induced decrease in serum adiponectin and reduces hepatic steatosis in rats[J].Hepatology,2009,49(5):1554-1562.

[21] KURZATKOWSKI D M,TROMBETTA L D.Maneb causes pro-oxidant effects in the hippocampus of Nrf2 knockout mice[J].Environmental Toxicology and Pharmacology,2013,36(2):427-436.

[22] WRUCK C J,FRAGOULIS A,GURZYNSKI A,et al.Role of oxidative stress in rheumatoid arthritis:insights from the Nrf2-knockout mice[J].Annals of the Rheumatic Diseases,2011,70(5):844-850.

[23] GIUDICE A,ARRA C,TURCO M C.Review of molecular mechanisms involved in the activation of the Nrf2-ARE signaling pathway by chemopreventive agents[J].Methods in Molecular Biology,2010,647:37-74.

[24] SINHA D,BISWAS J,BISHAYEE A.Nrf2-mediated redox signaling in arsenic carcinogenesis:a review[J].Archives of Toxicology,2013,87(2):383-396.

[25] STEPKOWSKI T M,KRUSZEWSKI M K.Molecular cross-talk between the NRF2/KEAP1 signaling pathway,autophagy,and apoptosis[J].Free Radical Biology and Medicine,2011,50(9):1186-1195.

[26] AGCA C A,TUZCU M,HAYIRLI A,et al.Taurine ameliorates neuropathy via regulating NF-κB and Nrf2/HO-1 signaling cascades in diabetic rats[J].Food and Chemical Toxicology,2014,71:116-121.

[27] JANG J S,PIAO S Y,CHA Y N,et al.Taurine chloramine activates Nrf2,increases HO-1 expression and protects cells from death caused by hydrogen peroxide[J].Journal of Clinical Biochemistry and Nutrition,2009,45(1):37-43.

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