Effects of Dietary Cation-Anion Difference Level on Plasma Calcium Concentration and Gastrointestinal Tract Vitamin D Receptor mRNA Expression Level of Mice during the Transition Period

XIN Hailiang, YANG Yi, DUAN Yongbang, ZHANG Jikun, ZHU Lunqin, TIAN Xingzhou, LI Cungang, TANG Zhihong, LIU Mingxue, WU Wenxuan

doi:10.3969/j.issn.1006-267x.2015.03.041

2015 , Vol. 27 >Issue 3: 997 - 1004

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.03.041

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Effects of Dietary Cation-Anion Difference Level on Plasma Calcium Concentration and Gastrointestinal Tract Vitamin D Receptor mRNA Expression Level of Mice during the Transition Period

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1. Qiandongnan National Polytechnic, Kaili 556000, China;
2. College of Animal Science, Guizhou University, Guiyang 550025, China;
3. Kaili Animal Husbandry and Veterinary Bureau of Guizhou Province, Kaili 564000, China;
4. Institute of Animal Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China;
5. Institute of New Rural Development, China Center for Western Development Capacity Research, Guizhou University, Guiyang 550025, China

Received date: 2014-09-29

Online published: 2015-03-18

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Abstract

This experiment was conducted to study the effects of dietary cation-anion difference (DCAD) level on plasma calcium concentration and gastrointestinal tract vitamin D receptor (VDR) mRNA expression level of mice during the transition period, and to exposit the mechanism of low DCAD level control of hypocalcemia. One hundred and twenty mice during the transition period were randomly allocated into 3 groups with 40 mice in each group, mice in the 3 groups were fed 3 diets with DCAD level at +300 (high DCAD level group, HD group), +150 (control group, CON group), and -150 (low DCAD level group, LD group), respectively. Ten mice for each group were sacrificed to collect blood sample for plasma calcium concentration analysis and gastrointestinal tract tissues (stomach, duodenum, jejunum, ileum, cecum, and colon) samples for VDR mRNA expression level detection at 4 time points: day of 20 (-20 d) and 5 (-5 d) before kidding, day of kidding (0 d), and day of 3 after kidding (+3 d). The results showed that the plasma calcium concentration in LD group was higher than that in HD and CON groups for the whole and individual blood sampling time (P<0.05). At -20 d, compared with HD and CON groups, the VDR mRNA expression level in jejunum, ileum and colon in LD group was significantly increased (P<0.05); at -5 d, the VDR mRNA expression level in jejunum and colon in LD group was significantly increased (P<0.05); at 0 d, the VDR mRNA expression level in duodenum in LD group was significantly increased (P<0.05); at +3 d, the VDR mRNA expression level in duodenum, jejunum and colon in LD group was significantly increased (P<0.05). The results indicated that low DCAD level can increase VDR mRNA expression level in gastrointestinal tract of mice during the transition period, and increase plasma calcium concentration, which was probably the mechanism of low DCAD preventing hypocalcemia.

Key words： dietary cation-anion difference; plasma calcium; vitamin D receptor; mice during the transition period

Cite this article

XIN Hailiang, YANG Yi, DUAN Yongbang, ZHANG Jikun, ZHU Lunqin, TIAN Xingzhou, LI Cungang, TANG Zhihong, LIU Mingxue, WU Wenxuan . Effects of Dietary Cation-Anion Difference Level on Plasma Calcium Concentration and Gastrointestinal Tract Vitamin D Receptor mRNA Expression Level of Mice during the Transition Period[J]. Chinese Journal of Animal Nutrition, 2015 , 27(3) : 997 -1004 . DOI: 10.3969/j.issn.1006-267x.2015.03.041

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