Effects of Dietary Zinc Level on Growth Performance, Immune and Antioxidant Capacity and Metallothionein-Ⅰ mRNA Expression of Geese Aged from 1 to 4 Weeks

WANG Baowei, CHEN Miaolu, WANG Binghan, ZHANG Ming, GE Wenhua, YUE Bin

doi:10.3969/j.issn.1006-267x.2015.04.011

2015 , Vol. 27 >Issue 4: 1076 - 1085

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.04.011

Poultry Nutrition

Effects of Dietary Zinc Level on Growth Performance, Immune and Antioxidant Capacity and Metallothionein-Ⅰ mRNA Expression of Geese Aged from 1 to 4 Weeks

Expand

1. Nutrition and Feed Research Laboratory of National Waterfowl Industry Technology System, Qingdao 266109, China;
2. Institute of High Quality Waterfowl, Qingdao Agricultural University, Qingdao 266109, China;
3. Lincoln University, Christchurch 85084, New Zealand

Received date: 2014-10-29

Online published: 2015-04-21

Fold

Abstract

This experiment was conducted to study the effects of dietary zinc level on growth performance, immune and antioxidant capacity and metallothionein-Ⅰ (MT-Ⅰ) mRNA expression of geese aged from 1 to 4 weeks, and to determine the optimal dietary level of zinc. A total of 360 one-day-old Qingnonghui geese were randomly divided into 6 groups with 6 replicates per group and 10 geese (half male and half female) per replicate. The dietary zinc levels in each group were 31.28 (group Ⅰ), 81.28 (group Ⅱ), 131.28 (group Ⅲ), 181.28 (group Ⅳ), 231.28 (group Ⅴ) and 281.28 (group Ⅵ) mg/kg, respectively. The experiment lasted for 28 days. The results showed as follows: 1) when dietary zinc level was 112.51 mg/kg, the average daily gain was the highest; while when dietary zinc level was 106.05 mg/kg, the ratio of feed to gain was the lowest. 2) With the increasing of dietary zinc level, the organ indices firstly increased, and then followed by a downward trend, the thymus index in group Ⅱ was significantly higher than that in group Ⅰ (P<0.01), the spleen index in groups Ⅱ and Ⅲ was significantly higher than that in group Ⅰ (P<0.01). 3) At 7 days after the immunization, the antibody titer in group Ⅳ was significantly higher than that in group Ⅰ (P<0.05); at 14 days after the immunization, the antibody titer in groups Ⅲ and Ⅳ was significantly higher than that in group Ⅰ (P<0.05). 4) Except the liver total antioxidant capacity (T-AOC) in group Ⅳ, the T-AOC and the activities of catalase (CAT), glutathione peroxidase (GSH-Px), copper zinc superoxide dismutase (CuZn-SOD) in groups Ⅲ and Ⅳ were significantly higher than those in group Ⅰ (P<0.05 or P<0.01), the malonaldehyde (MDA) content in group Ⅲ was the lowest. 5) The liver MT-Ⅰ mRNA expression in groups Ⅱ, Ⅲ and Ⅳ was significantly higher than that of group Ⅰ (P<0.05 or P<0.01). 6) The MT-Ⅰ mRNA expression was significantly correlated with body antioxidant capacity. In conclusion, dietary appropriate zinc level can improve the growth performance and immune and antioxidant capacity and MT-Ⅰ mRNA expression of geese. The requirement of zinc under the condition of high immunity and antioxidant capacity is higher than that under the condition of optimum growth performance.

Key words： zinc; geese; growth performance; immune; antioxidant; MT-Ⅰ

Cite this article

WANG Baowei, CHEN Miaolu, WANG Binghan, ZHANG Ming, GE Wenhua, YUE Bin . Effects of Dietary Zinc Level on Growth Performance, Immune and Antioxidant Capacity and Metallothionein-Ⅰ mRNA Expression of Geese Aged from 1 to 4 Weeks[J]. Chinese Journal of Animal Nutrition, 2015 , 27(4) : 1076 -1085 . DOI: 10.3969/j.issn.1006-267x.2015.04.011

References

[1] 韩新燕, 郭勇.微量元素锌与动物营养[J].饲料博览, 2000(10):26-27.

[2] 李大刚, 王宏, 周桂莲.微量元素锌的抗氧化作用研究[J].饲料研究, 2008(11):38-41.

[3] 丁小波, 文利新.微量元素锌与动物免疫[J].微量元素与健康研究, 2006, 23(3):55-57.

[4] 黄艳玲, 吕林, 李素芬, 等.0-21日龄肉仔鸡饲粮中锌适宜水平研究[J].畜牧兽医学报, 2008, 39(7):900-906.

[5] 孙淑霞, 赵晶, 李玉杰, 等.蛋氨酸螯合锌对绿头野鸭免疫器官指数及血液指标的影响[J].黑龙江畜牧兽医, 2008(9):42-43.

[6] 卢昊, 王春维, 周海, 等.谷氨酸锌对肉仔鸡生长性能、血清含锌酶活性及免疫器官指数的影响[J]中国饲料, 2010(1):24-26.

[7] 虞泽鹏, 乐国伟, 施用晖, 等.不同锌源对断奶小鼠生长及机体抗氧化能力的影响[J].畜牧与兽医, 2005, 37(4):1-3.

[8] COUSINS R J.Absorption, transport, and hepatic metabolism of copper and zinc:special reference to metallothionein and ceruloplasmin[J].Physiological Reviews, 1985, 65(2):238-309.

[9] 陈苗璐, 王宝维, 张名爱, 等.饲粮锌水平对鹅生长性能、血清生化指标及激素含量的影响[J].动物营养学报, 2013, 25(5) :1105-1112.

[10] NRC.Nutrient requirements of poultry[M].9th ed.Washington, D.C.:The National Academy Press, 1994.

[11] FRAKER P J, HAAS S M, LUECKE R W.Effect of zinc deficiency on the immune response of the young adult A/J mouse[J].The Journal of Nutrition, 1977, 107(10):1889-1895.

[12] 崔艳红, 刘保国, 王艳荣, 等.不同水平的蛋氨酸锌对肉用仔鸡生产性能和免疫的影响[J].饲料工业, 2009, 30(2):34-36.

[13] 张日俊, 周毓平, 黄燕, 等.锌对肉仔鸡免疫器官生长发育及免疫功能调节作用的研究[J].畜牧兽医学报, 1999, 30(6):504-512.

[14] 闫素梅, 郝永清, 史彬林, 等.日粮锌水平对肉仔鸡组织锌浓度及其生产性能与免疫机能的影响[J].饲料工业, 2002, 23(12):25-27.

[15] 冯望宝, 王安, 艾涛.不同锌水平对笼养育成蛋鸭生长性能及总抗氧化能力的影响[J].东北农业大学学报, 2007, 38(5):654-659.

[16] 钟映梅, 孙刚, 张俊峰, 等.不同锌水平对鹅血液抗氧化酶活性的影响[J].黑龙江畜牧兽医, 2007(3):44-45.

[17] 蒋宗勇, 刘小雁, 蒋守群, 等.43-63日龄黄羽肉鸡锌需要量的研究[J].中国农业科学, 2010, 43(20):4295-4302.

[18] SAHIN K, SMITH M O, ONDERCI M, et al.Supplementation of zinc from organic or inorganic source improves performance and antioxidant status of heat-distressed quail[J].Poultry Science, 2005, 84(6):882-887.

[19] 钟映梅, 王力强, 孙刚, 等.锌对鹅铜锌超氧化物歧化酶和丙二醛含量影响变化的研究[J].现代畜牧兽医, 2007(3):11-13.

[20] MARGOSHES M, VALLEE B L.A cadmium protein from equine kidney cortex[J].Journal of the American Chemical Society, 1957, 79(17):4813-4814.

[21] WEI D Y, ANDREWS G K.Molecular cloning of chicken metallothionein.Deduction of the complete amino add sequence and analysis of expression using cloned cDNA[J].Nucleic Acids Research, 1988, 16(2):537-553.

[22] 曹家银, 罗绪刚, DAVIS S R, 等.以组织锌、金属硫蛋白及其基因表达指标评价肉仔鸡对锌源的相对生物学利用率[J].畜牧兽医学报, 2003, 34(3):227-231.

[23] JOURDAN E, EMONET-PICCARDI N, DIDIER C, et al.Effects of cadmium and zinc on solar-simulated light-Ⅰrradiated cells:potential role of zinc-metallothionein in zinc-Ⅰnduced genoprotection[J].Archives of Biochemistry and Biophysics, 2002, 405(2):170-177.

[24] 刘湘新, 李丽立, 刘进辉, 等.金属硫蛋白对应激猪血清中某些酶活性的影响[J].中国兽医杂志, 2005, 41(12):7-8.

[25] 李峰, 石辉.锌金属硫蛋白对PM_2.5暴露的运动大鼠血清抗氧化酶及免疫指标的影响[J].环境科学学报, 32(2):465-471.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References